Differential Protein Expression among Two Different Ovine ARDS Phenotypes—A Preclinical Randomized Study
Despite decades of comprehensive research, Acute Respiratory Distress Syndrome (ARDS) remains a disease with high mortality and morbidity worldwide. The discovery of inflammatory subphenotypes in human ARDS provides a new approach to study the disease. In two different ovine ARDS lung injury models,...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/7/655 |
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| author | Karin Wildi Mahe Bouquet Carmen Ainola Samantha Livingstone Sebastiano Maria Colombo Silver Heinsar Noriko Sato Kei Sato Emily Wilson Gabriella Abbate Margaret R. Passmore Kieran Hyslop Keibun Liu Gianluigi Li Bassi Jacky Y. Suen John F. Fraser |
| author_facet | Karin Wildi Mahe Bouquet Carmen Ainola Samantha Livingstone Sebastiano Maria Colombo Silver Heinsar Noriko Sato Kei Sato Emily Wilson Gabriella Abbate Margaret R. Passmore Kieran Hyslop Keibun Liu Gianluigi Li Bassi Jacky Y. Suen John F. Fraser |
| author_sort | Karin Wildi |
| collection | DOAJ |
| description | Despite decades of comprehensive research, Acute Respiratory Distress Syndrome (ARDS) remains a disease with high mortality and morbidity worldwide. The discovery of inflammatory subphenotypes in human ARDS provides a new approach to study the disease. In two different ovine ARDS lung injury models, one induced by additional endotoxin infusion (phenotype 2), mimicking some key features as described in the human hyperinflammatory group, we aim to describe protein expression among the two different ovine models. Nine animals on mechanical ventilation were included in this study and were randomized into (a) phenotype 1, <i>n</i> = 5 (Ph1) and (b) phenotype 2, <i>n</i> = 4 (Ph2). Plasma was collected at baseline, 2, 6, 12, and 24 h. After protein extraction, data-independent SWATH-MS was applied to inspect protein abundance at baseline, 2, 6, 12, and 24 h. Cluster analysis revealed protein patterns emerging over the study observation time, more pronounced by the factor of time than different injury models of ARDS. A protein signature consisting of 33 proteins differentiated among Ph1/2 with high diagnostic accuracy. Applying network analysis, proteins involved in the inflammatory and defense response, complement and coagulation cascade, oxygen binding, and regulation of lipid metabolism were activated over time. Five proteins, namely LUM, CA2, KNG1, AGT, and IGJ, were more expressed in Ph2. |
| first_indexed | 2024-03-09T10:15:52Z |
| format | Article |
| id | doaj.art-e36760ef1d2c4b6a9afa09d409b5b65e |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T10:15:52Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-e36760ef1d2c4b6a9afa09d409b5b65e2023-12-01T22:26:48ZengMDPI AGMetabolites2218-19892022-07-0112765510.3390/metabo12070655Differential Protein Expression among Two Different Ovine ARDS Phenotypes—A Preclinical Randomized StudyKarin Wildi0Mahe Bouquet1Carmen Ainola2Samantha Livingstone3Sebastiano Maria Colombo4Silver Heinsar5Noriko Sato6Kei Sato7Emily Wilson8Gabriella Abbate9Margaret R. Passmore10Kieran Hyslop11Keibun Liu12Gianluigi Li Bassi13Jacky Y. Suen14John F. Fraser15Critical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaCritical Care Research Group, The Prince Charles Hospital, Brisbane 4032, AustraliaDespite decades of comprehensive research, Acute Respiratory Distress Syndrome (ARDS) remains a disease with high mortality and morbidity worldwide. The discovery of inflammatory subphenotypes in human ARDS provides a new approach to study the disease. In two different ovine ARDS lung injury models, one induced by additional endotoxin infusion (phenotype 2), mimicking some key features as described in the human hyperinflammatory group, we aim to describe protein expression among the two different ovine models. Nine animals on mechanical ventilation were included in this study and were randomized into (a) phenotype 1, <i>n</i> = 5 (Ph1) and (b) phenotype 2, <i>n</i> = 4 (Ph2). Plasma was collected at baseline, 2, 6, 12, and 24 h. After protein extraction, data-independent SWATH-MS was applied to inspect protein abundance at baseline, 2, 6, 12, and 24 h. Cluster analysis revealed protein patterns emerging over the study observation time, more pronounced by the factor of time than different injury models of ARDS. A protein signature consisting of 33 proteins differentiated among Ph1/2 with high diagnostic accuracy. Applying network analysis, proteins involved in the inflammatory and defense response, complement and coagulation cascade, oxygen binding, and regulation of lipid metabolism were activated over time. Five proteins, namely LUM, CA2, KNG1, AGT, and IGJ, were more expressed in Ph2.https://www.mdpi.com/2218-1989/12/7/655protein expression profilesSWATHAcute Respiratory Distress Syndrome (ARDS)phenotypesovine model |
| spellingShingle | Karin Wildi Mahe Bouquet Carmen Ainola Samantha Livingstone Sebastiano Maria Colombo Silver Heinsar Noriko Sato Kei Sato Emily Wilson Gabriella Abbate Margaret R. Passmore Kieran Hyslop Keibun Liu Gianluigi Li Bassi Jacky Y. Suen John F. Fraser Differential Protein Expression among Two Different Ovine ARDS Phenotypes—A Preclinical Randomized Study Metabolites protein expression profiles SWATH Acute Respiratory Distress Syndrome (ARDS) phenotypes ovine model |
| title | Differential Protein Expression among Two Different Ovine ARDS Phenotypes—A Preclinical Randomized Study |
| title_full | Differential Protein Expression among Two Different Ovine ARDS Phenotypes—A Preclinical Randomized Study |
| title_fullStr | Differential Protein Expression among Two Different Ovine ARDS Phenotypes—A Preclinical Randomized Study |
| title_full_unstemmed | Differential Protein Expression among Two Different Ovine ARDS Phenotypes—A Preclinical Randomized Study |
| title_short | Differential Protein Expression among Two Different Ovine ARDS Phenotypes—A Preclinical Randomized Study |
| title_sort | differential protein expression among two different ovine ards phenotypes a preclinical randomized study |
| topic | protein expression profiles SWATH Acute Respiratory Distress Syndrome (ARDS) phenotypes ovine model |
| url | https://www.mdpi.com/2218-1989/12/7/655 |
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