Simultaneous determination of cis- and trans-palmitoleic acid in rat serum by UPLC–MS/MS
Abstract Palmitoleic acid, a monounsaturated fatty acid which could affect glucose and lipid metabolism and reduce insulin resistance has two isomers, i.e. cis-palmitoleic acid (cPOA) and trans-palmitoleic acid (tPOA). However, the pharmacokinetic, metabolic transformation and structure–activity rel...
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| Format: | Article |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-20739-x |
| _version_ | 1811194940434153472 |
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| author | Wenwen Huang Yiping Zhang Liping Zhong Chunlong Sun Zaiwang Zhang |
| author_facet | Wenwen Huang Yiping Zhang Liping Zhong Chunlong Sun Zaiwang Zhang |
| author_sort | Wenwen Huang |
| collection | DOAJ |
| description | Abstract Palmitoleic acid, a monounsaturated fatty acid which could affect glucose and lipid metabolism and reduce insulin resistance has two isomers, i.e. cis-palmitoleic acid (cPOA) and trans-palmitoleic acid (tPOA). However, the pharmacokinetic, metabolic transformation and structure–activity relationship of the two isomers have not been reported. A precise and accurate ultra performance liquid chromatography–tandem mass spectroscopy (UPLC–MS/MS) method was developed to determine cPOA and tPOA simultaneously. Both the cPOA and tPOA were administered i.g. (intragastric gavage) to rats at 75 mg/kg. Serum samples were collected and analyzed for the two isomers by UPLC–MS/MS on a reverse-phase BDS C18 column equilibrated and eluted with water (A) and acetonitrile (B) at a flow rate of 0.3 mL/min. The calibration curves for cPOA and tPOA were linear over the range 0.1–12 μg/mL. Analytes were monitored by selected-reaction monitoring in negative electrospray ionization mode. The Tmax of cPOA was 0.94 ± 0.44 h and the Cmax 8.17 ± 1.97 μg/L, and the Tmax and Cmax of tPOA were 1.50 ± 0.98 h and 14.77 ± 11.91 μg/L, respectively. AUC0–24 h of cPOA and tPOA were 59.45 ± 29.83 and 113.88 ± 72.25 mg/L·h. The method was applied in pharmacokinetic study of cPOA and tPOA in rat serum successfully. Besides, the concentrations of cPOA and tPOA in rat serums were observed fluctuating with a consistent trend, which may be due to reciprocal bio-convert in the body. |
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| id | doaj.art-e36793a1c81c4a78a7bdbedcc1dcd782 |
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| language | English |
| last_indexed | 2024-04-12T00:35:44Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-e36793a1c81c4a78a7bdbedcc1dcd7822022-12-22T03:55:11ZengNature PortfolioScientific Reports2045-23222022-10-011211910.1038/s41598-022-20739-xSimultaneous determination of cis- and trans-palmitoleic acid in rat serum by UPLC–MS/MSWenwen Huang0Yiping Zhang1Liping Zhong2Chunlong Sun3Zaiwang Zhang4Binzhou Key Laboratory of Chemical Drug R&D and Quality Control, College of Biological and Environmental Engineering, Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Binzhou UniversityThe Third Institute of Oceanography, Ministry of Natural ResourcesTechnical Center of Xiamen Entry-Exit Inspection and Quarantine BureauBinzhou Key Laboratory of Chemical Drug R&D and Quality Control, College of Biological and Environmental Engineering, Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Binzhou UniversityBinzhou Key Laboratory of Chemical Drug R&D and Quality Control, College of Biological and Environmental Engineering, Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Binzhou UniversityAbstract Palmitoleic acid, a monounsaturated fatty acid which could affect glucose and lipid metabolism and reduce insulin resistance has two isomers, i.e. cis-palmitoleic acid (cPOA) and trans-palmitoleic acid (tPOA). However, the pharmacokinetic, metabolic transformation and structure–activity relationship of the two isomers have not been reported. A precise and accurate ultra performance liquid chromatography–tandem mass spectroscopy (UPLC–MS/MS) method was developed to determine cPOA and tPOA simultaneously. Both the cPOA and tPOA were administered i.g. (intragastric gavage) to rats at 75 mg/kg. Serum samples were collected and analyzed for the two isomers by UPLC–MS/MS on a reverse-phase BDS C18 column equilibrated and eluted with water (A) and acetonitrile (B) at a flow rate of 0.3 mL/min. The calibration curves for cPOA and tPOA were linear over the range 0.1–12 μg/mL. Analytes were monitored by selected-reaction monitoring in negative electrospray ionization mode. The Tmax of cPOA was 0.94 ± 0.44 h and the Cmax 8.17 ± 1.97 μg/L, and the Tmax and Cmax of tPOA were 1.50 ± 0.98 h and 14.77 ± 11.91 μg/L, respectively. AUC0–24 h of cPOA and tPOA were 59.45 ± 29.83 and 113.88 ± 72.25 mg/L·h. The method was applied in pharmacokinetic study of cPOA and tPOA in rat serum successfully. Besides, the concentrations of cPOA and tPOA in rat serums were observed fluctuating with a consistent trend, which may be due to reciprocal bio-convert in the body.https://doi.org/10.1038/s41598-022-20739-x |
| spellingShingle | Wenwen Huang Yiping Zhang Liping Zhong Chunlong Sun Zaiwang Zhang Simultaneous determination of cis- and trans-palmitoleic acid in rat serum by UPLC–MS/MS Scientific Reports |
| title | Simultaneous determination of cis- and trans-palmitoleic acid in rat serum by UPLC–MS/MS |
| title_full | Simultaneous determination of cis- and trans-palmitoleic acid in rat serum by UPLC–MS/MS |
| title_fullStr | Simultaneous determination of cis- and trans-palmitoleic acid in rat serum by UPLC–MS/MS |
| title_full_unstemmed | Simultaneous determination of cis- and trans-palmitoleic acid in rat serum by UPLC–MS/MS |
| title_short | Simultaneous determination of cis- and trans-palmitoleic acid in rat serum by UPLC–MS/MS |
| title_sort | simultaneous determination of cis and trans palmitoleic acid in rat serum by uplc ms ms |
| url | https://doi.org/10.1038/s41598-022-20739-x |
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