Isolation of a Highly Efficient Antigenic-Protein-Degrading <i>Bacillus amyloliquefaciens</i> and Assessment of Its Safety
The aims of this study were to screen and isolate a highly efficient strain from the rumen of a cow that can degrade the antigenic soy proteins in soybean meal (SBM) and improve the nutritional value of SBM by fermenting it with this strain. The safety of this strain was investigated with an acute o...
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/7/1144 |
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| author | Yang Li Baozhu Guo Chong Li Weiwei Wang Zhengke Wu Guohua Liu Huiyi Cai |
| author_facet | Yang Li Baozhu Guo Chong Li Weiwei Wang Zhengke Wu Guohua Liu Huiyi Cai |
| author_sort | Yang Li |
| collection | DOAJ |
| description | The aims of this study were to screen and isolate a highly efficient strain from the rumen of a cow that can degrade the antigenic soy proteins in soybean meal (SBM) and improve the nutritional value of SBM by fermenting it with this strain. The safety of this strain was investigated with an acute oral toxicity test. A <i>Bacillus amyloliquefaciens</i> strain was successfully screened with plate tests and fermentation. After solid state fermentation of SBM with B. <i>amyloliquefaciens</i> for 24 h, the amounts of glycinin and β-conglycinin, two major antigenic proteins in SBM, decreased by 92.32% and 85.05%, respectively. The crude protein content in the fermented soybean meal (FSBM) increased by 17.54% compared with that in SBM. Notably, the trichloroacetic-acid-soluble protein (TCA-SP) content, particularly small peptides and free amino acids, was 9.97-fold higher in FSBM than in SBM. The in vitro dry matter digestibility and digestible energy of SBM increased from 62.91% to 72.52% and from 10.42 MJ/kg to 13.37 MJ/kg (dry matter basis), respectively, after fermentation. The acute oral toxicity test suggested that the strain exerted no harmful effects on the relative organ weights, the morphological tissue structure, or the health of mice. These results indicate that the <i>B. amyloliquefaciens</i> strain isolated in this study is a safe strain for animals, and could be used to improve the nutritional quality of SBM by solid-state fermentation. |
| first_indexed | 2024-03-10T18:40:03Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T18:40:03Z |
| publishDate | 2020-07-01 |
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| series | Animals |
| spelling | doaj.art-e367a9a73a7447b891b371b47e21b2152023-11-20T05:59:29ZengMDPI AGAnimals2076-26152020-07-01107114410.3390/ani10071144Isolation of a Highly Efficient Antigenic-Protein-Degrading <i>Bacillus amyloliquefaciens</i> and Assessment of Its SafetyYang Li0Baozhu Guo1Chong Li2Weiwei Wang3Zhengke Wu4Guohua Liu5Huiyi Cai6The Key Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, Haidian District, Beijing 100081, ChinaThe Key Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, Haidian District, Beijing 100081, ChinaThe Key Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, Haidian District, Beijing 100081, ChinaThe Key Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, Haidian District, Beijing 100081, ChinaThe Key Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, Haidian District, Beijing 100081, ChinaThe Key Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, Haidian District, Beijing 100081, ChinaThe Key Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, Haidian District, Beijing 100081, ChinaThe aims of this study were to screen and isolate a highly efficient strain from the rumen of a cow that can degrade the antigenic soy proteins in soybean meal (SBM) and improve the nutritional value of SBM by fermenting it with this strain. The safety of this strain was investigated with an acute oral toxicity test. A <i>Bacillus amyloliquefaciens</i> strain was successfully screened with plate tests and fermentation. After solid state fermentation of SBM with B. <i>amyloliquefaciens</i> for 24 h, the amounts of glycinin and β-conglycinin, two major antigenic proteins in SBM, decreased by 92.32% and 85.05%, respectively. The crude protein content in the fermented soybean meal (FSBM) increased by 17.54% compared with that in SBM. Notably, the trichloroacetic-acid-soluble protein (TCA-SP) content, particularly small peptides and free amino acids, was 9.97-fold higher in FSBM than in SBM. The in vitro dry matter digestibility and digestible energy of SBM increased from 62.91% to 72.52% and from 10.42 MJ/kg to 13.37 MJ/kg (dry matter basis), respectively, after fermentation. The acute oral toxicity test suggested that the strain exerted no harmful effects on the relative organ weights, the morphological tissue structure, or the health of mice. These results indicate that the <i>B. amyloliquefaciens</i> strain isolated in this study is a safe strain for animals, and could be used to improve the nutritional quality of SBM by solid-state fermentation.https://www.mdpi.com/2076-2615/10/7/1144<i>B. amyloliquefaciens</i>fermentationsoybean mealantigenic proteinsafety assessment |
| spellingShingle | Yang Li Baozhu Guo Chong Li Weiwei Wang Zhengke Wu Guohua Liu Huiyi Cai Isolation of a Highly Efficient Antigenic-Protein-Degrading <i>Bacillus amyloliquefaciens</i> and Assessment of Its Safety Animals <i>B. amyloliquefaciens</i> fermentation soybean meal antigenic protein safety assessment |
| title | Isolation of a Highly Efficient Antigenic-Protein-Degrading <i>Bacillus amyloliquefaciens</i> and Assessment of Its Safety |
| title_full | Isolation of a Highly Efficient Antigenic-Protein-Degrading <i>Bacillus amyloliquefaciens</i> and Assessment of Its Safety |
| title_fullStr | Isolation of a Highly Efficient Antigenic-Protein-Degrading <i>Bacillus amyloliquefaciens</i> and Assessment of Its Safety |
| title_full_unstemmed | Isolation of a Highly Efficient Antigenic-Protein-Degrading <i>Bacillus amyloliquefaciens</i> and Assessment of Its Safety |
| title_short | Isolation of a Highly Efficient Antigenic-Protein-Degrading <i>Bacillus amyloliquefaciens</i> and Assessment of Its Safety |
| title_sort | isolation of a highly efficient antigenic protein degrading i bacillus amyloliquefaciens i and assessment of its safety |
| topic | <i>B. amyloliquefaciens</i> fermentation soybean meal antigenic protein safety assessment |
| url | https://www.mdpi.com/2076-2615/10/7/1144 |
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