Protective effects of fruit extract of Rosa canina and quercetin on human umbilical vein endothelial cell injury induced by hydrogen peroxide
Abstract The Nastaran plant, with the scientific name of Rosa canina, has been used since ancient times as a plant with medicinal properties. In the present study, human umbilical vein endothelial cells (HUVECs) were used to examine the protective effects of R. canina fruit extract (RCFE) and its fl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Food Science & Nutrition |
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| Online Access: | https://doi.org/10.1002/fsn3.3681 |
| _version_ | 1797389239270244352 |
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| author | Fatemeh Forouzanfar Zeynab Tabatabaei Seyed Ahmad Emami Zahra Ayati Zahra Tayarani‐Najaran |
| author_facet | Fatemeh Forouzanfar Zeynab Tabatabaei Seyed Ahmad Emami Zahra Ayati Zahra Tayarani‐Najaran |
| author_sort | Fatemeh Forouzanfar |
| collection | DOAJ |
| description | Abstract The Nastaran plant, with the scientific name of Rosa canina, has been used since ancient times as a plant with medicinal properties. In the present study, human umbilical vein endothelial cells (HUVECs) were used to examine the protective effects of R. canina fruit extract (RCFE) and its flavonoid ingredient (quercetin) against H2O2‐induced cell injury. RCFE (1.25–20 μg/mL) and quercetin (1.25–20 μM) were exposed to H2O2‐oxidizing agent (1 and 2 mM) and the protective effect was examined on HUVEC cells by Alamar Blue test. The amount of intracellular reactive oxygen species (ROS) was measured by using DCFDA reagent by fluorimetric method. The effects of RCFE and quercetin on cell apoptosis were studied by staining with hypotonic PI solution and flow cytometry. The amount of PARP and survivin involved in the apoptotic process was measured using the western blot analysis. The results of the Alamar Blue test showed that RCFE and quercetin could reduce the toxicity of H2O2. RCFE and quercetin were able to significantly increase cell viability against H2O2. Also, it was found that RCFE and quercetin reduced the production of ROS by H2O2. It was found that RCFE and quercetin reduced the apoptosis and sub‐G1 peak area in flow histogram after exposure of cells to H2O2. Based on western blot results, pretreatment with RCFE and quercetin could significantly increase survivin protein after exposure of cells to H2O2. Also, RCFE and quercetin could significantly reduce the amount of cleaved PARP after exposure of cells to H2O2. RCFE and its ingredient (quercetin) can be considered a promising source of phytochemicals in the prevention of cardiovascular diseases. |
| first_indexed | 2024-03-08T22:53:58Z |
| format | Article |
| id | doaj.art-e36b9a2d085040e297d687666b3a2ea1 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-03-08T22:53:58Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-e36b9a2d085040e297d687666b3a2ea12023-12-16T07:01:19ZengWileyFood Science & Nutrition2048-71772023-12-0111127618762510.1002/fsn3.3681Protective effects of fruit extract of Rosa canina and quercetin on human umbilical vein endothelial cell injury induced by hydrogen peroxideFatemeh Forouzanfar0Zeynab Tabatabaei1Seyed Ahmad Emami2Zahra Ayati3Zahra Tayarani‐Najaran4Department of Neuroscience, Faculty of Medicine Mashhad University of Medical Sciences Mashhad IranDepartment of Traditional Pharmacy, School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Traditional Pharmacy, School of Pharmacy Mashhad University of Medical Sciences Mashhad IranDepartment of Traditional Pharmacy, School of Pharmacy Mashhad University of Medical Sciences Mashhad IranTargeted Drug Delivery Research Center, Pharmaceutical Technology Institute Mashhad University of Medical Sciences Mashhad IranAbstract The Nastaran plant, with the scientific name of Rosa canina, has been used since ancient times as a plant with medicinal properties. In the present study, human umbilical vein endothelial cells (HUVECs) were used to examine the protective effects of R. canina fruit extract (RCFE) and its flavonoid ingredient (quercetin) against H2O2‐induced cell injury. RCFE (1.25–20 μg/mL) and quercetin (1.25–20 μM) were exposed to H2O2‐oxidizing agent (1 and 2 mM) and the protective effect was examined on HUVEC cells by Alamar Blue test. The amount of intracellular reactive oxygen species (ROS) was measured by using DCFDA reagent by fluorimetric method. The effects of RCFE and quercetin on cell apoptosis were studied by staining with hypotonic PI solution and flow cytometry. The amount of PARP and survivin involved in the apoptotic process was measured using the western blot analysis. The results of the Alamar Blue test showed that RCFE and quercetin could reduce the toxicity of H2O2. RCFE and quercetin were able to significantly increase cell viability against H2O2. Also, it was found that RCFE and quercetin reduced the production of ROS by H2O2. It was found that RCFE and quercetin reduced the apoptosis and sub‐G1 peak area in flow histogram after exposure of cells to H2O2. Based on western blot results, pretreatment with RCFE and quercetin could significantly increase survivin protein after exposure of cells to H2O2. Also, RCFE and quercetin could significantly reduce the amount of cleaved PARP after exposure of cells to H2O2. RCFE and its ingredient (quercetin) can be considered a promising source of phytochemicals in the prevention of cardiovascular diseases.https://doi.org/10.1002/fsn3.3681apoptosiscardiovascular diseasescytoprotectionoxidative damagequercetinRosa canina |
| spellingShingle | Fatemeh Forouzanfar Zeynab Tabatabaei Seyed Ahmad Emami Zahra Ayati Zahra Tayarani‐Najaran Protective effects of fruit extract of Rosa canina and quercetin on human umbilical vein endothelial cell injury induced by hydrogen peroxide Food Science & Nutrition apoptosis cardiovascular diseases cytoprotection oxidative damage quercetin Rosa canina |
| title | Protective effects of fruit extract of Rosa canina and quercetin on human umbilical vein endothelial cell injury induced by hydrogen peroxide |
| title_full | Protective effects of fruit extract of Rosa canina and quercetin on human umbilical vein endothelial cell injury induced by hydrogen peroxide |
| title_fullStr | Protective effects of fruit extract of Rosa canina and quercetin on human umbilical vein endothelial cell injury induced by hydrogen peroxide |
| title_full_unstemmed | Protective effects of fruit extract of Rosa canina and quercetin on human umbilical vein endothelial cell injury induced by hydrogen peroxide |
| title_short | Protective effects of fruit extract of Rosa canina and quercetin on human umbilical vein endothelial cell injury induced by hydrogen peroxide |
| title_sort | protective effects of fruit extract of rosa canina and quercetin on human umbilical vein endothelial cell injury induced by hydrogen peroxide |
| topic | apoptosis cardiovascular diseases cytoprotection oxidative damage quercetin Rosa canina |
| url | https://doi.org/10.1002/fsn3.3681 |
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