Motor coordination deficits in <it>Alpk1 </it>mutant mice with the inserted <it>piggyBac </it>transposon
<p>Abstract</p> <p>Background</p> <p>ALPK1 (α-kinase 1) is a member of an unconventional alpha-kinase family, and its biological function remains largely unknown. Here we report the phenotypic characterization of one mutant line, in which the <it>piggyBac </it&...
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| Format: | Article |
| Language: | English |
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BMC
2011-01-01
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| Series: | BMC Neuroscience |
| Online Access: | http://www.biomedcentral.com/1471-2202/12/1 |
| _version_ | 1818778837340651520 |
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| author | Xu Rener Chen Muyun |
| author_facet | Xu Rener Chen Muyun |
| author_sort | Xu Rener |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>ALPK1 (α-kinase 1) is a member of an unconventional alpha-kinase family, and its biological function remains largely unknown. Here we report the phenotypic characterization of one mutant line, in which the <it>piggyBac </it>(<it>PB</it>) transposon is inserted into the <it>Alpk1 </it>gene.</p> <p>Results</p> <p>The <it>piggyBac</it>(<it>PB</it>) insertion site in mutants was mapped to the first intron of the <it>Alpk1 </it>gene, resulting in the effective disruption of the intact <it>Alpk1 </it>transcript expression. The transposon-inserted <it>Alpk1 </it>homozygous mutants (<it>Alpk1<sup>PB/PB</sup></it>) displayed severe defects in motor coordination in a series of behavioral analysis, including dowel test, hanging wire test, rotarod analysis and footprint analysis. However, the cerebellar architecture, Purkinje cell morphology and electrophysiology of the Purkinje cells appeared normal in mutants. The motor coordination deficits in the <it>Alpk1<sup>PB/PB </sup></it>mice were rescued by transgenic mice expressing the full-length <it>Alpk1</it>-coding sequence under the control of the ubiquitous expression promoter.</p> <p>Conclusions</p> <p>Our results indicate that ALPK1 plays an important role in the regulation of motor coordination. <it>Alpk1<sup>PB/PB </sup></it>mice would be a useful model to provide a clue to the better understanding of the cellular and molecular mechanisms of ALPK1 in the control of fine motor activities.</p> |
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| format | Article |
| id | doaj.art-e36ccd413f12475ea62d500c47c02896 |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-12-18T11:51:04Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-e36ccd413f12475ea62d500c47c028962022-12-21T21:09:10ZengBMCBMC Neuroscience1471-22022011-01-01121110.1186/1471-2202-12-1Motor coordination deficits in <it>Alpk1 </it>mutant mice with the inserted <it>piggyBac </it>transposonXu RenerChen Muyun<p>Abstract</p> <p>Background</p> <p>ALPK1 (α-kinase 1) is a member of an unconventional alpha-kinase family, and its biological function remains largely unknown. Here we report the phenotypic characterization of one mutant line, in which the <it>piggyBac </it>(<it>PB</it>) transposon is inserted into the <it>Alpk1 </it>gene.</p> <p>Results</p> <p>The <it>piggyBac</it>(<it>PB</it>) insertion site in mutants was mapped to the first intron of the <it>Alpk1 </it>gene, resulting in the effective disruption of the intact <it>Alpk1 </it>transcript expression. The transposon-inserted <it>Alpk1 </it>homozygous mutants (<it>Alpk1<sup>PB/PB</sup></it>) displayed severe defects in motor coordination in a series of behavioral analysis, including dowel test, hanging wire test, rotarod analysis and footprint analysis. However, the cerebellar architecture, Purkinje cell morphology and electrophysiology of the Purkinje cells appeared normal in mutants. The motor coordination deficits in the <it>Alpk1<sup>PB/PB </sup></it>mice were rescued by transgenic mice expressing the full-length <it>Alpk1</it>-coding sequence under the control of the ubiquitous expression promoter.</p> <p>Conclusions</p> <p>Our results indicate that ALPK1 plays an important role in the regulation of motor coordination. <it>Alpk1<sup>PB/PB </sup></it>mice would be a useful model to provide a clue to the better understanding of the cellular and molecular mechanisms of ALPK1 in the control of fine motor activities.</p>http://www.biomedcentral.com/1471-2202/12/1 |
| spellingShingle | Xu Rener Chen Muyun Motor coordination deficits in <it>Alpk1 </it>mutant mice with the inserted <it>piggyBac </it>transposon BMC Neuroscience |
| title | Motor coordination deficits in <it>Alpk1 </it>mutant mice with the inserted <it>piggyBac </it>transposon |
| title_full | Motor coordination deficits in <it>Alpk1 </it>mutant mice with the inserted <it>piggyBac </it>transposon |
| title_fullStr | Motor coordination deficits in <it>Alpk1 </it>mutant mice with the inserted <it>piggyBac </it>transposon |
| title_full_unstemmed | Motor coordination deficits in <it>Alpk1 </it>mutant mice with the inserted <it>piggyBac </it>transposon |
| title_short | Motor coordination deficits in <it>Alpk1 </it>mutant mice with the inserted <it>piggyBac </it>transposon |
| title_sort | motor coordination deficits in it alpk1 it mutant mice with the inserted it piggybac it transposon |
| url | http://www.biomedcentral.com/1471-2202/12/1 |
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