Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to <i>Helicobacter pylori</i> Infection and <i>cagA</i> Transfection
<i>Background and Objectives:</i> The role of α-enolase (ENO1) in <i>Helicobacter pylori</i>-related gastric lesions might be a critical factor in the pathogenesis, but remains undefined. <i>Materials and Methods:</i> This study investigated the differential expre...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/1648-9144/58/10/1453 |
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| author | Feiyan Yu Mengya He Jian Li Haiyan Wang Shuaiyin Chen Xiaojuan Zhang Huijuan Zhang Guangcai Duan Rongguang Zhang |
| author_facet | Feiyan Yu Mengya He Jian Li Haiyan Wang Shuaiyin Chen Xiaojuan Zhang Huijuan Zhang Guangcai Duan Rongguang Zhang |
| author_sort | Feiyan Yu |
| collection | DOAJ |
| description | <i>Background and Objectives:</i> The role of α-enolase (ENO1) in <i>Helicobacter pylori</i>-related gastric lesions might be a critical factor in the pathogenesis, but remains undefined. <i>Materials and Methods:</i> This study investigated the differential expression of α-enolase in clinical gastric specimens and cultured normal/cancer cells in response to <i>H. pylori</i> (<i>cagA+</i>) infection and <i>cagA</i> transfection using qPCR, Western blots and histochemical methods. <i>Results:</i> A total of 172 gastric specimens were collected from 142 patients, the former comprising chronic superficial gastritis (CSG), precancerous diseases (PCDs, including atrophic gastritis, intestinal metaplasia and dysplasia) and gastric cancer (GC) cases. Among the CSG and PCD cases, the <i>H. pylori</i>-infected group had significantly elevated ENO1 mRNA levels compared with the uninfected group. In the GC cases, differential ENO1 expressions were detected between the cancer tissues and the paracancerous tissues. Notably, significant difference was first detected between the GC cell (AGS) and the normal cell (GES-1) as a response of ENO1 to <i>H. pylori</i> infection and <i>cagA</i> transfection. <i>Conclusions:</i> This report reveals that ENO1 expression is associated with <i>H. pylori</i> infection, <i>cagA</i> transfection, co-culture duration, multiplicity of infection, gastric normal/cancerous cell lines and cellular differentiation. The findings may be crucial bases for further ascertaining <i>H. pylori</i> pathogenic mechanism and formulating novel therapeutic and diagnostic strategies. |
| first_indexed | 2024-03-09T19:50:47Z |
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| language | English |
| last_indexed | 2024-03-09T19:50:47Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Medicina |
| spelling | doaj.art-e371a043e0794312b791d9e12282ebf82023-11-24T01:11:26ZengMDPI AGMedicina1010-660X1648-91442022-10-015810145310.3390/medicina58101453Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to <i>Helicobacter pylori</i> Infection and <i>cagA</i> TransfectionFeiyan Yu0Mengya He1Jian Li2Haiyan Wang3Shuaiyin Chen4Xiaojuan Zhang5Huijuan Zhang6Guangcai Duan7Rongguang Zhang8The First Affiliated Hospital, International School of Public Health and One Health, Hainan Medical University, Haikou 570102, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, ChinaHenan Provincial People’s Hospital, Zhengzhou University, Zhengzhou 450003, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Basic Medical Sciences, Henan University of Science and Technology, Luoyang 471000, ChinaThe First Affiliated Hospital, International School of Public Health and One Health, Hainan Medical University, Haikou 570102, ChinaThe First Affiliated Hospital, International School of Public Health and One Health, Hainan Medical University, Haikou 570102, ChinaThe First Affiliated Hospital, International School of Public Health and One Health, Hainan Medical University, Haikou 570102, China<i>Background and Objectives:</i> The role of α-enolase (ENO1) in <i>Helicobacter pylori</i>-related gastric lesions might be a critical factor in the pathogenesis, but remains undefined. <i>Materials and Methods:</i> This study investigated the differential expression of α-enolase in clinical gastric specimens and cultured normal/cancer cells in response to <i>H. pylori</i> (<i>cagA+</i>) infection and <i>cagA</i> transfection using qPCR, Western blots and histochemical methods. <i>Results:</i> A total of 172 gastric specimens were collected from 142 patients, the former comprising chronic superficial gastritis (CSG), precancerous diseases (PCDs, including atrophic gastritis, intestinal metaplasia and dysplasia) and gastric cancer (GC) cases. Among the CSG and PCD cases, the <i>H. pylori</i>-infected group had significantly elevated ENO1 mRNA levels compared with the uninfected group. In the GC cases, differential ENO1 expressions were detected between the cancer tissues and the paracancerous tissues. Notably, significant difference was first detected between the GC cell (AGS) and the normal cell (GES-1) as a response of ENO1 to <i>H. pylori</i> infection and <i>cagA</i> transfection. <i>Conclusions:</i> This report reveals that ENO1 expression is associated with <i>H. pylori</i> infection, <i>cagA</i> transfection, co-culture duration, multiplicity of infection, gastric normal/cancerous cell lines and cellular differentiation. The findings may be crucial bases for further ascertaining <i>H. pylori</i> pathogenic mechanism and formulating novel therapeutic and diagnostic strategies.https://www.mdpi.com/1648-9144/58/10/1453<i>Helicobacter pylori</i>α-enolasegastric cancercytotoxin associated gene Aprecancer lesion |
| spellingShingle | Feiyan Yu Mengya He Jian Li Haiyan Wang Shuaiyin Chen Xiaojuan Zhang Huijuan Zhang Guangcai Duan Rongguang Zhang Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to <i>Helicobacter pylori</i> Infection and <i>cagA</i> Transfection Medicina <i>Helicobacter pylori</i> α-enolase gastric cancer cytotoxin associated gene A precancer lesion |
| title | Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to <i>Helicobacter pylori</i> Infection and <i>cagA</i> Transfection |
| title_full | Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to <i>Helicobacter pylori</i> Infection and <i>cagA</i> Transfection |
| title_fullStr | Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to <i>Helicobacter pylori</i> Infection and <i>cagA</i> Transfection |
| title_full_unstemmed | Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to <i>Helicobacter pylori</i> Infection and <i>cagA</i> Transfection |
| title_short | Differential Expression of α-Enolase in Clinical Gastric Tissues and Cultured Normal/Cancer Cells in Response to <i>Helicobacter pylori</i> Infection and <i>cagA</i> Transfection |
| title_sort | differential expression of α enolase in clinical gastric tissues and cultured normal cancer cells in response to i helicobacter pylori i infection and i caga i transfection |
| topic | <i>Helicobacter pylori</i> α-enolase gastric cancer cytotoxin associated gene A precancer lesion |
| url | https://www.mdpi.com/1648-9144/58/10/1453 |
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