Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches.
The follicle-associated epithelium (FAE) selectively transports prions, viruses, pathogenic bacteria, commensal microflora, and even secretory IgA (SIgA)-immune complexes from the intestinal lumen to underlying gut-associated lymphoid tissues like Peyer's patches. The FAE consists of a single l...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4404297?pdf=render |
| _version_ | 1819266314015866880 |
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| author | Maria Olga Hernandez Nicholas J Mantis |
| author_facet | Maria Olga Hernandez Nicholas J Mantis |
| author_sort | Maria Olga Hernandez |
| collection | DOAJ |
| description | The follicle-associated epithelium (FAE) selectively transports prions, viruses, pathogenic bacteria, commensal microflora, and even secretory IgA (SIgA)-immune complexes from the intestinal lumen to underlying gut-associated lymphoid tissues like Peyer's patches. The FAE consists of a single layer of columnar epithelial cells that includes enterocytes and M (microfold) cells, intermingled with dendritic cells (DCs), macrophages, and naïve and memory B and T lymphocytes. In this report we describe a population of IgA+ cells that reside within and immediately below the FAE in mouse Peyer's patches. Immunofluorescence microscopy analysis indicated that the FAE-associated IgA+ cells were negative for surface antigen markers specific for B cells (B220), T cells (CD3), DCs (CD11c), and plasma cells (CD138). The IgA+ cells were also negative Ki-67 and IRF4, indicating that they are not mature B cells or plasma cells. The IgA+ cells were, however, often found in close proximity to DCs, leading us to speculate that the population of IgA+ cells in the FAE constitutes an atypical subset of B cells involved in mucosal antigen surveillance and/or immune recall. |
| first_indexed | 2024-12-23T20:59:18Z |
| format | Article |
| id | doaj.art-e37231f44d9d41bc86a4756a71675741 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T20:59:18Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
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| spelling | doaj.art-e37231f44d9d41bc86a4756a716757412022-12-21T17:31:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012411110.1371/journal.pone.0124111Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches.Maria Olga HernandezNicholas J MantisThe follicle-associated epithelium (FAE) selectively transports prions, viruses, pathogenic bacteria, commensal microflora, and even secretory IgA (SIgA)-immune complexes from the intestinal lumen to underlying gut-associated lymphoid tissues like Peyer's patches. The FAE consists of a single layer of columnar epithelial cells that includes enterocytes and M (microfold) cells, intermingled with dendritic cells (DCs), macrophages, and naïve and memory B and T lymphocytes. In this report we describe a population of IgA+ cells that reside within and immediately below the FAE in mouse Peyer's patches. Immunofluorescence microscopy analysis indicated that the FAE-associated IgA+ cells were negative for surface antigen markers specific for B cells (B220), T cells (CD3), DCs (CD11c), and plasma cells (CD138). The IgA+ cells were also negative Ki-67 and IRF4, indicating that they are not mature B cells or plasma cells. The IgA+ cells were, however, often found in close proximity to DCs, leading us to speculate that the population of IgA+ cells in the FAE constitutes an atypical subset of B cells involved in mucosal antigen surveillance and/or immune recall.http://europepmc.org/articles/PMC4404297?pdf=render |
| spellingShingle | Maria Olga Hernandez Nicholas J Mantis Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches. PLoS ONE |
| title | Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches. |
| title_full | Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches. |
| title_fullStr | Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches. |
| title_full_unstemmed | Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches. |
| title_short | Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches. |
| title_sort | phenotypic analysis of a population of iga cells in the follicle associated epithelium of mouse peyer s patches |
| url | http://europepmc.org/articles/PMC4404297?pdf=render |
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