The Effects of <i>Tachykinin1</i> Gene Products on Prepubertal Dabry’s Sturgeon (<i>Acipenser dabrynus</i>) Pituitary Hormone Secretion and Gene Expression
As an ancient and endangered species unique to the Yangtze River in China, the wild population of the Dabry’s sturgeon has become scarce. Due to the long time till the first sexual maturity of Dabry’s sturgeon, the population of artificially bred Dabry’s sturgeon recovered slowly. As a member of the...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/2/227 |
| _version_ | 1797344782960295936 |
|---|---|
| author | Kan Xiao Hongtao Huang Xuetao Shi Tingting Shu Xu Cheng Hejun Du Jing Yang |
| author_facet | Kan Xiao Hongtao Huang Xuetao Shi Tingting Shu Xu Cheng Hejun Du Jing Yang |
| author_sort | Kan Xiao |
| collection | DOAJ |
| description | As an ancient and endangered species unique to the Yangtze River in China, the wild population of the Dabry’s sturgeon has become scarce. Due to the long time till the first sexual maturity of Dabry’s sturgeon, the population of artificially bred Dabry’s sturgeon recovered slowly. As a member of the tachykinin family, TAC1 has been reported to have a variety of functions in mammals such as pain control, smooth muscle contraction and reproductive cycle regulation, but the function of Tac1 in fish has been rarely reported. In this study, we synthesized two <i>tac1</i> gene products, Substance P (SP) and neurokinin A (NKA), and further verified the effect of two <i>tac1</i> gene products on the secretion of related hormones in the pituitary of Dabry’s Sturgeon by intraperitoneal injection and co-culture of primary cells. Expression studies revealed that the newly cloned <i>tac1</i> were mainly distributed in the hypothalamus and pituitary tissue of the brain. In prepubertal Dabry’s sturgeon, this study showed that the two gonadotropins’ mRNA levels in pituitary tissue can be significantly increased by SP and NKA through intraperitoneal injection, and the LH protein level in serum was also increased. Further study showed that both NKA and SP could promote the two gonadotropins’ mRNA expression in pituitary cells of Dabry’s sturgeon. In addition, we explored the optimal dose and time of SP and NKA on pituitary cells is 24 h and over 10 nM. These results, as a whole, suggested that <i>tac1</i> gene products play an important role in gonadotropin release and gonadal development in prepubertal Dabry’s sturgeon. |
| first_indexed | 2024-03-08T11:08:54Z |
| format | Article |
| id | doaj.art-e3743f758c8f43689a193b366bde8f16 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T11:08:54Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-e3743f758c8f43689a193b366bde8f162024-01-26T14:31:13ZengMDPI AGAnimals2076-26152024-01-0114222710.3390/ani14020227The Effects of <i>Tachykinin1</i> Gene Products on Prepubertal Dabry’s Sturgeon (<i>Acipenser dabrynus</i>) Pituitary Hormone Secretion and Gene ExpressionKan Xiao0Hongtao Huang1Xuetao Shi2Tingting Shu3Xu Cheng4Hejun Du5Jing Yang6Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, ChinaAs an ancient and endangered species unique to the Yangtze River in China, the wild population of the Dabry’s sturgeon has become scarce. Due to the long time till the first sexual maturity of Dabry’s sturgeon, the population of artificially bred Dabry’s sturgeon recovered slowly. As a member of the tachykinin family, TAC1 has been reported to have a variety of functions in mammals such as pain control, smooth muscle contraction and reproductive cycle regulation, but the function of Tac1 in fish has been rarely reported. In this study, we synthesized two <i>tac1</i> gene products, Substance P (SP) and neurokinin A (NKA), and further verified the effect of two <i>tac1</i> gene products on the secretion of related hormones in the pituitary of Dabry’s Sturgeon by intraperitoneal injection and co-culture of primary cells. Expression studies revealed that the newly cloned <i>tac1</i> were mainly distributed in the hypothalamus and pituitary tissue of the brain. In prepubertal Dabry’s sturgeon, this study showed that the two gonadotropins’ mRNA levels in pituitary tissue can be significantly increased by SP and NKA through intraperitoneal injection, and the LH protein level in serum was also increased. Further study showed that both NKA and SP could promote the two gonadotropins’ mRNA expression in pituitary cells of Dabry’s sturgeon. In addition, we explored the optimal dose and time of SP and NKA on pituitary cells is 24 h and over 10 nM. These results, as a whole, suggested that <i>tac1</i> gene products play an important role in gonadotropin release and gonadal development in prepubertal Dabry’s sturgeon.https://www.mdpi.com/2076-2615/14/2/227endangered speciesneuropeptideSPNKA |
| spellingShingle | Kan Xiao Hongtao Huang Xuetao Shi Tingting Shu Xu Cheng Hejun Du Jing Yang The Effects of <i>Tachykinin1</i> Gene Products on Prepubertal Dabry’s Sturgeon (<i>Acipenser dabrynus</i>) Pituitary Hormone Secretion and Gene Expression Animals endangered species neuropeptide SP NKA |
| title | The Effects of <i>Tachykinin1</i> Gene Products on Prepubertal Dabry’s Sturgeon (<i>Acipenser dabrynus</i>) Pituitary Hormone Secretion and Gene Expression |
| title_full | The Effects of <i>Tachykinin1</i> Gene Products on Prepubertal Dabry’s Sturgeon (<i>Acipenser dabrynus</i>) Pituitary Hormone Secretion and Gene Expression |
| title_fullStr | The Effects of <i>Tachykinin1</i> Gene Products on Prepubertal Dabry’s Sturgeon (<i>Acipenser dabrynus</i>) Pituitary Hormone Secretion and Gene Expression |
| title_full_unstemmed | The Effects of <i>Tachykinin1</i> Gene Products on Prepubertal Dabry’s Sturgeon (<i>Acipenser dabrynus</i>) Pituitary Hormone Secretion and Gene Expression |
| title_short | The Effects of <i>Tachykinin1</i> Gene Products on Prepubertal Dabry’s Sturgeon (<i>Acipenser dabrynus</i>) Pituitary Hormone Secretion and Gene Expression |
| title_sort | effects of i tachykinin1 i gene products on prepubertal dabry s sturgeon i acipenser dabrynus i pituitary hormone secretion and gene expression |
| topic | endangered species neuropeptide SP NKA |
| url | https://www.mdpi.com/2076-2615/14/2/227 |
| work_keys_str_mv | AT kanxiao theeffectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT hongtaohuang theeffectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT xuetaoshi theeffectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT tingtingshu theeffectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT xucheng theeffectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT hejundu theeffectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT jingyang theeffectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT kanxiao effectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT hongtaohuang effectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT xuetaoshi effectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT tingtingshu effectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT xucheng effectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT hejundu effectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression AT jingyang effectsofitachykinin1igeneproductsonprepubertaldabryssturgeoniacipenserdabrynusipituitaryhormonesecretionandgeneexpression |