Validation of an analytical method for the determination of fatty acids in sheep blood serum samples
Low-density dispersive liquid-liquid microextraction with a subsequent esterification step was proposed in this study for the determination of linoleic acid and stearic acid in sheep blood serum samples. The method developed aimed to quickly and efficiently extract and esterify both fatty acids with...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2023-05-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000400375&lng=en&tlng=en |
| _version_ | 1797816831582404608 |
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| author | Matheus Julien Ferreira Bazzana Juliana Garcia Gabriela Françozo Vilela Cleber Nogueira Borges Letícia Rodrigues Faria Nadja Gomes Alves Adelir Aparecida Saczk |
| author_facet | Matheus Julien Ferreira Bazzana Juliana Garcia Gabriela Françozo Vilela Cleber Nogueira Borges Letícia Rodrigues Faria Nadja Gomes Alves Adelir Aparecida Saczk |
| author_sort | Matheus Julien Ferreira Bazzana |
| collection | DOAJ |
| description | Low-density dispersive liquid-liquid microextraction with a subsequent esterification step was proposed in this study for the determination of linoleic acid and stearic acid in sheep blood serum samples. The method developed aimed to quickly and efficiently extract and esterify both fatty acids with subsequent analysis by gas chromatography/flame ionization detection. The extraction method was optimized with Scheffé’s polynomial model for three-component mixtures. The method was validated according to the parameters established by Eurachem Guide (2014). The optimal extraction conditions for LD-DLLME were 1400 µL of dispersion medium (MgCl2 0.017%), 400 µL of extractor solvent (toluene) and 1200 µL of dispersion solvent (methanol). The method performance showed adequate selectivity, sensitivity and precision to be applied to real samples, with an average recovery of 98.54% for linoleic acid and 103.83% for stearic acid. LD-DLLME was superior to the traditional method of analysis, which has been used until now for the determination of fatty acids in blood serum samples from ruminants. The analysis of real samples showed that the developed method is efficient for monitoring these substances in ruminants. |
| first_indexed | 2024-03-13T08:43:32Z |
| format | Article |
| id | doaj.art-e374821178254d50bdd611940296180c |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-03-13T08:43:32Z |
| publishDate | 2023-05-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-e374821178254d50bdd611940296180c2023-05-30T07:39:52ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642023-05-0146437538010.21577/0100-4042.20230009Validation of an analytical method for the determination of fatty acids in sheep blood serum samplesMatheus Julien Ferreira Bazzanahttps://orcid.org/0000-0003-3241-389XJuliana GarciaGabriela Françozo VilelaCleber Nogueira BorgesLetícia Rodrigues FariaNadja Gomes AlvesAdelir Aparecida SaczkLow-density dispersive liquid-liquid microextraction with a subsequent esterification step was proposed in this study for the determination of linoleic acid and stearic acid in sheep blood serum samples. The method developed aimed to quickly and efficiently extract and esterify both fatty acids with subsequent analysis by gas chromatography/flame ionization detection. The extraction method was optimized with Scheffé’s polynomial model for three-component mixtures. The method was validated according to the parameters established by Eurachem Guide (2014). The optimal extraction conditions for LD-DLLME were 1400 µL of dispersion medium (MgCl2 0.017%), 400 µL of extractor solvent (toluene) and 1200 µL of dispersion solvent (methanol). The method performance showed adequate selectivity, sensitivity and precision to be applied to real samples, with an average recovery of 98.54% for linoleic acid and 103.83% for stearic acid. LD-DLLME was superior to the traditional method of analysis, which has been used until now for the determination of fatty acids in blood serum samples from ruminants. The analysis of real samples showed that the developed method is efficient for monitoring these substances in ruminants.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000400375&lng=en&tlng=enLinoleic acidStearic acidchemometricsminiaturized extraction technique. |
| spellingShingle | Matheus Julien Ferreira Bazzana Juliana Garcia Gabriela Françozo Vilela Cleber Nogueira Borges Letícia Rodrigues Faria Nadja Gomes Alves Adelir Aparecida Saczk Validation of an analytical method for the determination of fatty acids in sheep blood serum samples Química Nova Linoleic acid Stearic acid chemometrics miniaturized extraction technique. |
| title | Validation of an analytical method for the determination of fatty acids in sheep blood serum samples |
| title_full | Validation of an analytical method for the determination of fatty acids in sheep blood serum samples |
| title_fullStr | Validation of an analytical method for the determination of fatty acids in sheep blood serum samples |
| title_full_unstemmed | Validation of an analytical method for the determination of fatty acids in sheep blood serum samples |
| title_short | Validation of an analytical method for the determination of fatty acids in sheep blood serum samples |
| title_sort | validation of an analytical method for the determination of fatty acids in sheep blood serum samples |
| topic | Linoleic acid Stearic acid chemometrics miniaturized extraction technique. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000400375&lng=en&tlng=en |
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