Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura)

Abstract Two allopatric populations of Brazilian diploid and tetraploid Odontophrynus americanus species complex, both from São Paulo state, had their blood hemoglobin biochemically analyzed. In addition, these specimens were cytogenetically characterized. Biochemical characterization of hemoglobin...

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Main Authors: Aurora M. Cianciarullo, Claudia R. Bonini-Domingos, Luiz D. Vizotto, Leonardo S. Kobashi, Maria-Luiza Beçak, Willy Beçak
Format: Article
Language:English
Published: Sociedade Brasileira de Genética
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019005026107&lng=en&tlng=en
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author Aurora M. Cianciarullo
Claudia R. Bonini-Domingos
Luiz D. Vizotto
Leonardo S. Kobashi
Maria-Luiza Beçak
Willy Beçak
author_facet Aurora M. Cianciarullo
Claudia R. Bonini-Domingos
Luiz D. Vizotto
Leonardo S. Kobashi
Maria-Luiza Beçak
Willy Beçak
author_sort Aurora M. Cianciarullo
collection DOAJ
description Abstract Two allopatric populations of Brazilian diploid and tetraploid Odontophrynus americanus species complex, both from São Paulo state, had their blood hemoglobin biochemically analyzed. In addition, these specimens were cytogenetically characterized. Biochemical characterization of hemoglobin expression showed a distinct banding pattern between the allopatric specimens. Besides this, two distinct phenotypes, not linked to ploidy, sex, or age, were observed in adult animals of both populations. Phenotype A exhibits dark-colored body with small papillae, ogival-shaped jaw with reduced interpupillary distance and shorter hind limbs. Phenotype B shows yellowish-colored body with larger papillae, arch-shaped jaw with broader interpupillary distance and longer hind limbs. Intermediate phenotypes were also found. Considering the geographical isolation of both populations, differences in chromosomal secondary constrictions and distinct hemoglobins banding patterns, these data indicate that 2n and 4n populations represent cryptic species in the O. americanus species complex. The observed phenotypic diversity can be interpreted as population genetic variability. Eventually future data may indicate a probable beginning of speciation in these Brazilian frogs. Such inter- and intrapopulational differentiation/speciation process indicates that O. americanus species complex taxonomy deserves further evaluation by genomics and metabarcoding communities, also considering the pattern of hemoglobin expression, in South American frogs.
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spelling doaj.art-e37b37241f034a5a907a840d602933302022-12-22T02:02:58ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-468510.1590/1678-4685-gmb-2017-0260S1415-47572019005026107Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura)Aurora M. CianciarulloClaudia R. Bonini-DomingosLuiz D. VizottoLeonardo S. KobashiMaria-Luiza BeçakWilly BeçakAbstract Two allopatric populations of Brazilian diploid and tetraploid Odontophrynus americanus species complex, both from São Paulo state, had their blood hemoglobin biochemically analyzed. In addition, these specimens were cytogenetically characterized. Biochemical characterization of hemoglobin expression showed a distinct banding pattern between the allopatric specimens. Besides this, two distinct phenotypes, not linked to ploidy, sex, or age, were observed in adult animals of both populations. Phenotype A exhibits dark-colored body with small papillae, ogival-shaped jaw with reduced interpupillary distance and shorter hind limbs. Phenotype B shows yellowish-colored body with larger papillae, arch-shaped jaw with broader interpupillary distance and longer hind limbs. Intermediate phenotypes were also found. Considering the geographical isolation of both populations, differences in chromosomal secondary constrictions and distinct hemoglobins banding patterns, these data indicate that 2n and 4n populations represent cryptic species in the O. americanus species complex. The observed phenotypic diversity can be interpreted as population genetic variability. Eventually future data may indicate a probable beginning of speciation in these Brazilian frogs. Such inter- and intrapopulational differentiation/speciation process indicates that O. americanus species complex taxonomy deserves further evaluation by genomics and metabarcoding communities, also considering the pattern of hemoglobin expression, in South American frogs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019005026107&lng=en&tlng=enAnuracryptic specieshemoglobin differentiationpolyploidywhole-genome duplication
spellingShingle Aurora M. Cianciarullo
Claudia R. Bonini-Domingos
Luiz D. Vizotto
Leonardo S. Kobashi
Maria-Luiza Beçak
Willy Beçak
Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura)
Genetics and Molecular Biology
Anura
cryptic species
hemoglobin differentiation
polyploidy
whole-genome duplication
title Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura)
title_full Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura)
title_fullStr Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura)
title_full_unstemmed Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura)
title_short Whole-genome duplication and hemoglobin differentiation traits between allopatric populations of Brazilian Odontophrynus americanus species complex (Amphibia, Anura)
title_sort whole genome duplication and hemoglobin differentiation traits between allopatric populations of brazilian odontophrynus americanus species complex amphibia anura
topic Anura
cryptic species
hemoglobin differentiation
polyploidy
whole-genome duplication
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019005026107&lng=en&tlng=en
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