Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips
Abstract The influence of variations of gravity, either hypergravity or microgravity, on the brain of astronauts is a major concern for long journeys in space, to the Moon or to Mars, or simply long-duration missions on the ISS (International Space Station). Monitoring brain activity, before and aft...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | npj Microgravity |
| Online Access: | https://doi.org/10.1038/s41526-024-00355-x |
| _version_ | 1797273778024087552 |
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| author | Pierre-Ewen Lecoq Chloé Dupuis Xavier Mousset Xavier Benoit-Gonnin Jean-Michel Peyrin Jean-Luc Aider |
| author_facet | Pierre-Ewen Lecoq Chloé Dupuis Xavier Mousset Xavier Benoit-Gonnin Jean-Michel Peyrin Jean-Luc Aider |
| author_sort | Pierre-Ewen Lecoq |
| collection | DOAJ |
| description | Abstract The influence of variations of gravity, either hypergravity or microgravity, on the brain of astronauts is a major concern for long journeys in space, to the Moon or to Mars, or simply long-duration missions on the ISS (International Space Station). Monitoring brain activity, before and after ISS missions already demonstrated important and long term effects on the brains of astronauts. In this study, we focus on the influence of gravity variations at the cellular level on primary hippocampal neurons. A dedicated setup has been designed and built to perform live calcium imaging during parabolic flights. During a CNES (Centre National d’Etudes Spatiales) parabolic flight campaign, we were able to observe and monitor the calcium activity of 2D networks of neurons inside microfluidic devices during gravity changes over different parabolas. Our preliminary results clearly indicate a modification of the calcium activity associated to variations of gravity. |
| first_indexed | 2024-03-07T14:49:04Z |
| format | Article |
| id | doaj.art-e37c3cd9ecee4bf2aecb94e210d568a2 |
| institution | Directory Open Access Journal |
| issn | 2373-8065 |
| language | English |
| last_indexed | 2024-03-07T14:49:04Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Microgravity |
| spelling | doaj.art-e37c3cd9ecee4bf2aecb94e210d568a22024-03-05T19:51:14ZengNature Portfolionpj Microgravity2373-80652024-02-0110111010.1038/s41526-024-00355-xInfluence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chipsPierre-Ewen Lecoq0Chloé Dupuis1Xavier Mousset2Xavier Benoit-Gonnin3Jean-Michel Peyrin4Jean-Luc Aider5PMMH, ESPCI Paris - PSLPMMH, ESPCI Paris - PSLPMMH, ESPCI Paris - PSLPMMH, ESPCI Paris - PSLNeurosciences Paris Seine IBPS, UMR8246, Inserm U1130, Sorbonne UniversityPMMH, ESPCI Paris - PSLAbstract The influence of variations of gravity, either hypergravity or microgravity, on the brain of astronauts is a major concern for long journeys in space, to the Moon or to Mars, or simply long-duration missions on the ISS (International Space Station). Monitoring brain activity, before and after ISS missions already demonstrated important and long term effects on the brains of astronauts. In this study, we focus on the influence of gravity variations at the cellular level on primary hippocampal neurons. A dedicated setup has been designed and built to perform live calcium imaging during parabolic flights. During a CNES (Centre National d’Etudes Spatiales) parabolic flight campaign, we were able to observe and monitor the calcium activity of 2D networks of neurons inside microfluidic devices during gravity changes over different parabolas. Our preliminary results clearly indicate a modification of the calcium activity associated to variations of gravity.https://doi.org/10.1038/s41526-024-00355-x |
| spellingShingle | Pierre-Ewen Lecoq Chloé Dupuis Xavier Mousset Xavier Benoit-Gonnin Jean-Michel Peyrin Jean-Luc Aider Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips npj Microgravity |
| title | Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips |
| title_full | Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips |
| title_fullStr | Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips |
| title_full_unstemmed | Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips |
| title_short | Influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips |
| title_sort | influence of microgravity on spontaneous calcium activity of primary hippocampal neurons grown in microfluidic chips |
| url | https://doi.org/10.1038/s41526-024-00355-x |
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