Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass Spectrometry
The aim of this study was to develop and to validate a toxicological untargeted screening relying on LC-HRMS in meconium including the detection of the four main classes of drugs of abuse (DoA; amphetamines, cannabinoids, opioids and cocaine). The method was then applied to 29 real samples. Analyses...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/10/2/55 |
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| author | Atakan Hernandez Valerie Lacroze Natalia Doudka Jenny Becam Carole Pourriere-Fabiani Bruno Lacarelle Caroline Solas Nicolas Fabresse |
| author_facet | Atakan Hernandez Valerie Lacroze Natalia Doudka Jenny Becam Carole Pourriere-Fabiani Bruno Lacarelle Caroline Solas Nicolas Fabresse |
| author_sort | Atakan Hernandez |
| collection | DOAJ |
| description | The aim of this study was to develop and to validate a toxicological untargeted screening relying on LC-HRMS in meconium including the detection of the four main classes of drugs of abuse (DoA; amphetamines, cannabinoids, opioids and cocaine). The method was then applied to 29 real samples. Analyses were performed with a liquid chromatography system coupled to a benchtop Orbitrap operating in a data-dependent analysis. The sample amount was 300 mg of meconium extracted twice by solid phase extraction following two distinct procedures. Raw data were processed using the Compound Discoverer 3.2 software (Thermo). The method was evaluated and validated on 15 compounds (6-MAM, morphine, buprenorphine, norbuprenorphine, methadone, EDDP, amphetamine, MDA, MDMA, methamphetamine, cocaine, benzoylecgonine, THC, 11-OH-THC, THC-COOH). Limits of detection were between 0.5 and 5 pg/mg and limits of identification between 5 and 50 pg/mg. Mean matrix effect was between −79 and −19% (<i>n</i> = 6) and mean overall recovery between 18 and 73% (<i>n</i> = 6) at 100 pg/mg. The application allows the detection of 88 substances, including 47 pharmaceuticals and 15 pharmaceutical metabolites, cocaine and its metabolites, THC and its metabolites, and natural (morphine, codeine) and synthetic (methadone, buprenorphine, tramadol, norfentanyl) opioids. This method is now used routinely for toxicological screening in high-risk pregnancies |
| first_indexed | 2024-03-09T20:56:55Z |
| format | Article |
| id | doaj.art-e37cc435d4d84710be39033483cc6e82 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T20:56:55Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-e37cc435d4d84710be39033483cc6e822023-11-23T22:20:07ZengMDPI AGToxics2305-63042022-01-011025510.3390/toxics10020055Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass SpectrometryAtakan Hernandez0Valerie Lacroze1Natalia Doudka2Jenny Becam3Carole Pourriere-Fabiani4Bruno Lacarelle5Caroline Solas6Nicolas Fabresse7Laboratory of Pharmacokinetics and Toxicology, La Timone University Hospital, 264 Rue Saint Pierre, CEDEX 5, 13385 Marseille, FranceDepartment of Neonatalogy, La Conception University Hospital, CEDEX 5, 13385 Marseille, FranceDepartment of Clinical Pharmacology and Pharmacovigilance, La Timone University Hospital, 264 Rue Saint Pierre, CEDEX 5, 13385 Marseille, FranceLaboratory of Pharmacokinetics and Toxicology, La Timone University Hospital, 264 Rue Saint Pierre, CEDEX 5, 13385 Marseille, FranceLaboratory of Pharmacokinetics and Toxicology, La Timone University Hospital, 264 Rue Saint Pierre, CEDEX 5, 13385 Marseille, FranceLaboratory of Pharmacokinetics and Toxicology, La Timone University Hospital, 264 Rue Saint Pierre, CEDEX 5, 13385 Marseille, FranceLaboratory of Pharmacokinetics and Toxicology, La Timone University Hospital, 264 Rue Saint Pierre, CEDEX 5, 13385 Marseille, FranceLaboratory of Pharmacokinetics and Toxicology, La Timone University Hospital, 264 Rue Saint Pierre, CEDEX 5, 13385 Marseille, FranceThe aim of this study was to develop and to validate a toxicological untargeted screening relying on LC-HRMS in meconium including the detection of the four main classes of drugs of abuse (DoA; amphetamines, cannabinoids, opioids and cocaine). The method was then applied to 29 real samples. Analyses were performed with a liquid chromatography system coupled to a benchtop Orbitrap operating in a data-dependent analysis. The sample amount was 300 mg of meconium extracted twice by solid phase extraction following two distinct procedures. Raw data were processed using the Compound Discoverer 3.2 software (Thermo). The method was evaluated and validated on 15 compounds (6-MAM, morphine, buprenorphine, norbuprenorphine, methadone, EDDP, amphetamine, MDA, MDMA, methamphetamine, cocaine, benzoylecgonine, THC, 11-OH-THC, THC-COOH). Limits of detection were between 0.5 and 5 pg/mg and limits of identification between 5 and 50 pg/mg. Mean matrix effect was between −79 and −19% (<i>n</i> = 6) and mean overall recovery between 18 and 73% (<i>n</i> = 6) at 100 pg/mg. The application allows the detection of 88 substances, including 47 pharmaceuticals and 15 pharmaceutical metabolites, cocaine and its metabolites, THC and its metabolites, and natural (morphine, codeine) and synthetic (methadone, buprenorphine, tramadol, norfentanyl) opioids. This method is now used routinely for toxicological screening in high-risk pregnancieshttps://www.mdpi.com/2305-6304/10/2/55meconiummass spectrometrytoxicologydrug of abuseorbitrapnewborn |
| spellingShingle | Atakan Hernandez Valerie Lacroze Natalia Doudka Jenny Becam Carole Pourriere-Fabiani Bruno Lacarelle Caroline Solas Nicolas Fabresse Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass Spectrometry Toxics meconium mass spectrometry toxicology drug of abuse orbitrap newborn |
| title | Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass Spectrometry |
| title_full | Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass Spectrometry |
| title_fullStr | Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass Spectrometry |
| title_full_unstemmed | Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass Spectrometry |
| title_short | Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass Spectrometry |
| title_sort | determination of prenatal substance exposure using meconium and orbitrap mass spectrometry |
| topic | meconium mass spectrometry toxicology drug of abuse orbitrap newborn |
| url | https://www.mdpi.com/2305-6304/10/2/55 |
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