Development of bioanalytical parameters for the standardization of Zingiber officinale

Objective: To develop noval bioanalytical parameters for standardization of Zingiber officinale (Z. officinale) extract. Methods: Phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and fingerprint analysis through HPTLC method were carried out in the present investiga...

Full description

Bibliographic Details
Main Authors: Dinesh K. Patel, S.P. Dhanabal
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2013-01-01
Series:Journal of Acute Disease
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2221618913601134
Description
Summary:Objective: To develop noval bioanalytical parameters for standardization of Zingiber officinale (Z. officinale) extract. Methods: Phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and fingerprint analysis through HPTLC method were carried out in the present investigation. Moreover quantitative analysis of rutin and quercetin in Z. officinale extract through HPTLC techniques were also performed in the present investigation. Results: Phytochemical analysis showed the presence of alkaloid, carbohydrate, tannin, steroid, triterpenoid, glycoside, triterpenoid, saponin, flavonoid, amino acid and protein. The total phenol and flavonoid content were found to be 0.59% and 4.50%. For quantitative analysis through HPTLC techniques, optimization of solvent system was done and the content of quercetin and rutin in the extract was found to be 3.22% (w/w) and 0.65% (w/w) respectively. Conclusions: These above mentioned parameters can be used as an important tool for the standardization of Z. officinale extract.
ISSN:2221-6189