Development of bioanalytical parameters for the standardization of Zingiber officinale
Objective: To develop noval bioanalytical parameters for standardization of Zingiber officinale (Z. officinale) extract. Methods: Phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and fingerprint analysis through HPTLC method were carried out in the present investiga...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Journal of Acute Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221618913601134 |
| _version_ | 1818932787533578240 |
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| author | Dinesh K. Patel S.P. Dhanabal |
| author_facet | Dinesh K. Patel S.P. Dhanabal |
| author_sort | Dinesh K. Patel |
| collection | DOAJ |
| description | Objective: To develop noval bioanalytical parameters for standardization of Zingiber officinale (Z. officinale) extract.
Methods: Phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and fingerprint analysis through HPTLC method were carried out in the present investigation. Moreover quantitative analysis of rutin and quercetin in Z. officinale extract through HPTLC techniques were also performed in the present investigation.
Results: Phytochemical analysis showed the presence of alkaloid, carbohydrate, tannin, steroid, triterpenoid, glycoside, triterpenoid, saponin, flavonoid, amino acid and protein. The total phenol and flavonoid content were found to be 0.59% and 4.50%. For quantitative analysis through HPTLC techniques, optimization of solvent system was done and the content of quercetin and rutin in the extract was found to be 3.22% (w/w) and 0.65% (w/w) respectively.
Conclusions: These above mentioned parameters can be used as an important tool for the standardization of Z. officinale extract. |
| first_indexed | 2024-12-20T04:38:02Z |
| format | Article |
| id | doaj.art-e382bb0dfab644349236ad4dfde8d1a1 |
| institution | Directory Open Access Journal |
| issn | 2221-6189 |
| language | English |
| last_indexed | 2024-12-20T04:38:02Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Acute Disease |
| spelling | doaj.art-e382bb0dfab644349236ad4dfde8d1a12022-12-21T19:53:12ZengWolters Kluwer Medknow PublicationsJournal of Acute Disease2221-61892013-01-012213413610.1016/S2221-6189(13)60113-4Development of bioanalytical parameters for the standardization of Zingiber officinaleDinesh K. Patel0S.P. Dhanabal1Department of Pharmaceutics, Indian Institute of Technology, Banaras Hindu University, Varanasi-221005, IndiaJ.S.S. College of Pharmacy, Ooty-643 001, IndiaObjective: To develop noval bioanalytical parameters for standardization of Zingiber officinale (Z. officinale) extract. Methods: Phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and fingerprint analysis through HPTLC method were carried out in the present investigation. Moreover quantitative analysis of rutin and quercetin in Z. officinale extract through HPTLC techniques were also performed in the present investigation. Results: Phytochemical analysis showed the presence of alkaloid, carbohydrate, tannin, steroid, triterpenoid, glycoside, triterpenoid, saponin, flavonoid, amino acid and protein. The total phenol and flavonoid content were found to be 0.59% and 4.50%. For quantitative analysis through HPTLC techniques, optimization of solvent system was done and the content of quercetin and rutin in the extract was found to be 3.22% (w/w) and 0.65% (w/w) respectively. Conclusions: These above mentioned parameters can be used as an important tool for the standardization of Z. officinale extract.http://www.sciencedirect.com/science/article/pii/S2221618913601134HPTLCPhytochemical analysisStandardizationZinziber officinale |
| spellingShingle | Dinesh K. Patel S.P. Dhanabal Development of bioanalytical parameters for the standardization of Zingiber officinale Journal of Acute Disease HPTLC Phytochemical analysis Standardization Zinziber officinale |
| title | Development of bioanalytical parameters for the standardization of Zingiber officinale |
| title_full | Development of bioanalytical parameters for the standardization of Zingiber officinale |
| title_fullStr | Development of bioanalytical parameters for the standardization of Zingiber officinale |
| title_full_unstemmed | Development of bioanalytical parameters for the standardization of Zingiber officinale |
| title_short | Development of bioanalytical parameters for the standardization of Zingiber officinale |
| title_sort | development of bioanalytical parameters for the standardization of zingiber officinale |
| topic | HPTLC Phytochemical analysis Standardization Zinziber officinale |
| url | http://www.sciencedirect.com/science/article/pii/S2221618913601134 |
| work_keys_str_mv | AT dineshkpatel developmentofbioanalyticalparametersforthestandardizationofzingiberofficinale AT spdhanabal developmentofbioanalyticalparametersforthestandardizationofzingiberofficinale |