Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milk
Rifaximin (RIF) is an important drug in clinical veterinary, and its residue in milk might pose a potential threat to human health. To monitor RIF residue in milk, a europium nanospheres-based time-resolved fluorescence immunoassay (TRFIA) was developed. The TRFIA is based on the competitive reactio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2019.1669538 |
| _version_ | 1811286645364752384 |
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| author | Licai Ma Zhanhui Wang Hebing Liu Congming Wu Yafang Ding Kai Wen |
| author_facet | Licai Ma Zhanhui Wang Hebing Liu Congming Wu Yafang Ding Kai Wen |
| author_sort | Licai Ma |
| collection | DOAJ |
| description | Rifaximin (RIF) is an important drug in clinical veterinary, and its residue in milk might pose a potential threat to human health. To monitor RIF residue in milk, a europium nanospheres-based time-resolved fluorescence immunoassay (TRFIA) was developed. The TRFIA is based on the competitive reaction between RIF-ovalbumin (OVA) and the target RIF in milk for binding to the Eu-Nanospheres-mAbs. The limit of detection (LOD) and limit of quantity (LOQ) were 3.05 and 6.63 ng/mL, respectively. The recoveries of the TRFIA to detect RIF in spiked milk samples ranged from 73.7% to 103.9%, and the interday and intraday variation were respectively less than 12.1% and 10.5%. A quantitative comparison of TRFIA and UPLC-MS/MS method exhibited a significant correlation (R2 = 0.9725) at the six various spiked levels. These results indicated that the TRFIA we developed was applicable for the detection of large numbers of milk samples. |
| first_indexed | 2024-04-13T03:04:44Z |
| format | Article |
| id | doaj.art-e38495539e53474cbbd58f0ee1ba035b |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-04-13T03:04:44Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-e38495539e53474cbbd58f0ee1ba035b2022-12-22T03:05:19ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-013011135114710.1080/09540105.2019.16695381669538Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milkLicai Ma0Zhanhui Wang1Hebing Liu2Congming Wu3Yafang Ding4Kai Wen5College of Veterinary Medicine, China Agricultural UniversityChina Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and SafetyBeijing WDWK Biotechnology Company, Ltd.College of Veterinary Medicine, China Agricultural UniversityBeijing WDWK Biotechnology Company, Ltd.College of Veterinary Medicine, China Agricultural UniversityRifaximin (RIF) is an important drug in clinical veterinary, and its residue in milk might pose a potential threat to human health. To monitor RIF residue in milk, a europium nanospheres-based time-resolved fluorescence immunoassay (TRFIA) was developed. The TRFIA is based on the competitive reaction between RIF-ovalbumin (OVA) and the target RIF in milk for binding to the Eu-Nanospheres-mAbs. The limit of detection (LOD) and limit of quantity (LOQ) were 3.05 and 6.63 ng/mL, respectively. The recoveries of the TRFIA to detect RIF in spiked milk samples ranged from 73.7% to 103.9%, and the interday and intraday variation were respectively less than 12.1% and 10.5%. A quantitative comparison of TRFIA and UPLC-MS/MS method exhibited a significant correlation (R2 = 0.9725) at the six various spiked levels. These results indicated that the TRFIA we developed was applicable for the detection of large numbers of milk samples.http://dx.doi.org/10.1080/09540105.2019.1669538rifaximineu-nanospheresmonoclonal antibodyimmunoassaymilk |
| spellingShingle | Licai Ma Zhanhui Wang Hebing Liu Congming Wu Yafang Ding Kai Wen Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milk Food and Agricultural Immunology rifaximin eu-nanospheres monoclonal antibody immunoassay milk |
| title | Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milk |
| title_full | Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milk |
| title_fullStr | Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milk |
| title_full_unstemmed | Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milk |
| title_short | Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milk |
| title_sort | monoclonal antibody production and the development of a quantitative time resolved fluoroimmunoassay for rifaximin in milk |
| topic | rifaximin eu-nanospheres monoclonal antibody immunoassay milk |
| url | http://dx.doi.org/10.1080/09540105.2019.1669538 |
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