Diatom-Derived Polyunsaturated Aldehydes Are Unlikely to Influence the Microbiota Composition of Laboratory-Cultured Diatoms
Diatom-derived oxylipins, including polyunsaturated aldehydes (PUA), are considered to have infochemical, allelochemical and bacteriostatic properties, with plausible roles as grazing deterrents and regulators of inter- and intraspecific competition. However, the extent and mechanisms of how PUA inf...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-03-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/10/3/29 |
| _version_ | 1811299576651448320 |
|---|---|
| author | Chloe L. Eastabrook Paul Whitworth Georgina Robinson Gary S. Caldwell |
| author_facet | Chloe L. Eastabrook Paul Whitworth Georgina Robinson Gary S. Caldwell |
| author_sort | Chloe L. Eastabrook |
| collection | DOAJ |
| description | Diatom-derived oxylipins, including polyunsaturated aldehydes (PUA), are considered to have infochemical, allelochemical and bacteriostatic properties, with plausible roles as grazing deterrents and regulators of inter- and intraspecific competition. However, the extent and mechanisms of how PUA influence diatom−bacteria interactions remain unresolved. In this study, impacts on the diversity of the associated bacterial communities (microbiota) of two contrasting <i>Skeletonema marinoi</i> strains (a PUA and a non-PUA producer) were investigated under three nitrate conditions in batch culture. Further, the response of the culture microbiota was studied when spiked with PUA at ecologically relevant concentrations (86nM octadienal and 290nM heptadienal). Of the 741 identified OTUs, Proteobacteria was the most abundant phylum (62.10%), followed by Bacteroidetes (12.33%) and Firmicutes (6.11%). <i>Escherichia/Shigella</i> were the most abundant genera for all treatments. Similar communities were present in both spiked and non-spiked cultures suggesting they can tolerate PUA exposure at realistic concentrations. This study suggests that PUA are not major drivers of diatom−bacteria interactions in laboratory cultures. |
| first_indexed | 2024-04-13T06:37:54Z |
| format | Article |
| id | doaj.art-e384de9d79164a49b5aa5d15e3ff582b |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-04-13T06:37:54Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-e384de9d79164a49b5aa5d15e3ff582b2022-12-22T02:57:51ZengMDPI AGLife2075-17292020-03-011032910.3390/life10030029life10030029Diatom-Derived Polyunsaturated Aldehydes Are Unlikely to Influence the Microbiota Composition of Laboratory-Cultured DiatomsChloe L. Eastabrook0Paul Whitworth1Georgina Robinson2Gary S. Caldwell3School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UKSchool of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UKScottish Association for Marine Science, Scottish Marine Institute, Oban PA37 1QA, UKSchool of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UKDiatom-derived oxylipins, including polyunsaturated aldehydes (PUA), are considered to have infochemical, allelochemical and bacteriostatic properties, with plausible roles as grazing deterrents and regulators of inter- and intraspecific competition. However, the extent and mechanisms of how PUA influence diatom−bacteria interactions remain unresolved. In this study, impacts on the diversity of the associated bacterial communities (microbiota) of two contrasting <i>Skeletonema marinoi</i> strains (a PUA and a non-PUA producer) were investigated under three nitrate conditions in batch culture. Further, the response of the culture microbiota was studied when spiked with PUA at ecologically relevant concentrations (86nM octadienal and 290nM heptadienal). Of the 741 identified OTUs, Proteobacteria was the most abundant phylum (62.10%), followed by Bacteroidetes (12.33%) and Firmicutes (6.11%). <i>Escherichia/Shigella</i> were the most abundant genera for all treatments. Similar communities were present in both spiked and non-spiked cultures suggesting they can tolerate PUA exposure at realistic concentrations. This study suggests that PUA are not major drivers of diatom−bacteria interactions in laboratory cultures.https://www.mdpi.com/2075-1729/10/3/29bacterioplankton interactions<i>skeletonema marinoi</i>polyunsaturated aldehydesnitrogen alterationsalgae microbiome |
| spellingShingle | Chloe L. Eastabrook Paul Whitworth Georgina Robinson Gary S. Caldwell Diatom-Derived Polyunsaturated Aldehydes Are Unlikely to Influence the Microbiota Composition of Laboratory-Cultured Diatoms Life bacterioplankton interactions <i>skeletonema marinoi</i> polyunsaturated aldehydes nitrogen alterations algae microbiome |
| title | Diatom-Derived Polyunsaturated Aldehydes Are Unlikely to Influence the Microbiota Composition of Laboratory-Cultured Diatoms |
| title_full | Diatom-Derived Polyunsaturated Aldehydes Are Unlikely to Influence the Microbiota Composition of Laboratory-Cultured Diatoms |
| title_fullStr | Diatom-Derived Polyunsaturated Aldehydes Are Unlikely to Influence the Microbiota Composition of Laboratory-Cultured Diatoms |
| title_full_unstemmed | Diatom-Derived Polyunsaturated Aldehydes Are Unlikely to Influence the Microbiota Composition of Laboratory-Cultured Diatoms |
| title_short | Diatom-Derived Polyunsaturated Aldehydes Are Unlikely to Influence the Microbiota Composition of Laboratory-Cultured Diatoms |
| title_sort | diatom derived polyunsaturated aldehydes are unlikely to influence the microbiota composition of laboratory cultured diatoms |
| topic | bacterioplankton interactions <i>skeletonema marinoi</i> polyunsaturated aldehydes nitrogen alterations algae microbiome |
| url | https://www.mdpi.com/2075-1729/10/3/29 |
| work_keys_str_mv | AT chloeleastabrook diatomderivedpolyunsaturatedaldehydesareunlikelytoinfluencethemicrobiotacompositionoflaboratorycultureddiatoms AT paulwhitworth diatomderivedpolyunsaturatedaldehydesareunlikelytoinfluencethemicrobiotacompositionoflaboratorycultureddiatoms AT georginarobinson diatomderivedpolyunsaturatedaldehydesareunlikelytoinfluencethemicrobiotacompositionoflaboratorycultureddiatoms AT garyscaldwell diatomderivedpolyunsaturatedaldehydesareunlikelytoinfluencethemicrobiotacompositionoflaboratorycultureddiatoms |