Bio-acceptability of wearable sensors: a mechanistic study towards evaluating ionic leaching induced cellular inflammation
Abstract The recent need for remote health wellness monitoring has led to the extensive use of wearable sensors. Owing to their increased use, these sensors are required to exhibit both functionality and safety to the user. A major component in the fabrication of these sensors and their associated c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13810-0 |
| _version_ | 1811227249750310912 |
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| author | Pulak Bhushan Vivek Kamat Ishita Abrol Ajeet Kaushik Shekhar Bhansali |
| author_facet | Pulak Bhushan Vivek Kamat Ishita Abrol Ajeet Kaushik Shekhar Bhansali |
| author_sort | Pulak Bhushan |
| collection | DOAJ |
| description | Abstract The recent need for remote health wellness monitoring has led to the extensive use of wearable sensors. Owing to their increased use, these sensors are required to exhibit both functionality and safety to the user. A major component in the fabrication of these sensors and their associated circuitry is the use of metallic/organic conductive inks. However, very less is known about the interfacial and molecular interactions of these inks with biological matter as they can result in an inflammatory reaction to the user. Significant efforts are thus needed to explore and improve the bio-acceptability of such conductive ink-based wearable sensors. The present study investigates the biocompatibility of encapsulated and non-encapsulated wearable electrochemical sensors used for sensing uric acid as a biomarker for wound healing fabricated using screen-printing technique. Ionic release of metallic ions was investigated first to understand the susceptibility of the conductive inks towards ionic leaching when in contact with a fluid. Time-lapse investigation using ICPS (inductive couple plasma spectroscopy) shows a high concentration (607.31 ppb) of leached silver (Ag+) ions from the non-encapsulated sensors. The cell viability data suggests a 2.5-fold improvement in the sensor biocompatibility for an encapsulated sensor. While the carbon ink shows negligible effect on cell viability, the silver ink elicits significant decrease (< 50%) in cell viability at concentrations higher than 2 mg ml-1. The toxicity pathway of these sensors was further determined to be through the generation of reactive oxygen species resulting in over 20% apoptotic cell death. Our results show that the lower biocompatibility of the non-encapsulated sensor attributes to the higher leaching of Ag+ ions from the printed inks which elicits several different inflammatory pathways. This work highlights the importance biocompatibility evaluation of the material used in sensor fabrication to develop safe and sustainable sensors for long-term applications. |
| first_indexed | 2024-04-12T09:39:27Z |
| format | Article |
| id | doaj.art-e3853efe1f0f4b3382499c47cd642191 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T09:39:27Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e3853efe1f0f4b3382499c47cd6421912022-12-22T03:38:09ZengNature PortfolioScientific Reports2045-23222022-06-011211910.1038/s41598-022-13810-0Bio-acceptability of wearable sensors: a mechanistic study towards evaluating ionic leaching induced cellular inflammationPulak Bhushan0Vivek Kamat1Ishita Abrol2Ajeet Kaushik3Shekhar Bhansali4Department of Electrical and Computer Engineering, Florida International UniversityDepartment of Electrical and Computer Engineering, Florida International UniversityDepartment of Electrical and Computer Engineering, Florida International UniversityNanoBiotech Lab, Health System Engineering, Department of Environmental Engineering, Florida Polytechnic UniversityDepartment of Electrical and Computer Engineering, Florida International UniversityAbstract The recent need for remote health wellness monitoring has led to the extensive use of wearable sensors. Owing to their increased use, these sensors are required to exhibit both functionality and safety to the user. A major component in the fabrication of these sensors and their associated circuitry is the use of metallic/organic conductive inks. However, very less is known about the interfacial and molecular interactions of these inks with biological matter as they can result in an inflammatory reaction to the user. Significant efforts are thus needed to explore and improve the bio-acceptability of such conductive ink-based wearable sensors. The present study investigates the biocompatibility of encapsulated and non-encapsulated wearable electrochemical sensors used for sensing uric acid as a biomarker for wound healing fabricated using screen-printing technique. Ionic release of metallic ions was investigated first to understand the susceptibility of the conductive inks towards ionic leaching when in contact with a fluid. Time-lapse investigation using ICPS (inductive couple plasma spectroscopy) shows a high concentration (607.31 ppb) of leached silver (Ag+) ions from the non-encapsulated sensors. The cell viability data suggests a 2.5-fold improvement in the sensor biocompatibility for an encapsulated sensor. While the carbon ink shows negligible effect on cell viability, the silver ink elicits significant decrease (< 50%) in cell viability at concentrations higher than 2 mg ml-1. The toxicity pathway of these sensors was further determined to be through the generation of reactive oxygen species resulting in over 20% apoptotic cell death. Our results show that the lower biocompatibility of the non-encapsulated sensor attributes to the higher leaching of Ag+ ions from the printed inks which elicits several different inflammatory pathways. This work highlights the importance biocompatibility evaluation of the material used in sensor fabrication to develop safe and sustainable sensors for long-term applications.https://doi.org/10.1038/s41598-022-13810-0 |
| spellingShingle | Pulak Bhushan Vivek Kamat Ishita Abrol Ajeet Kaushik Shekhar Bhansali Bio-acceptability of wearable sensors: a mechanistic study towards evaluating ionic leaching induced cellular inflammation Scientific Reports |
| title | Bio-acceptability of wearable sensors: a mechanistic study towards evaluating ionic leaching induced cellular inflammation |
| title_full | Bio-acceptability of wearable sensors: a mechanistic study towards evaluating ionic leaching induced cellular inflammation |
| title_fullStr | Bio-acceptability of wearable sensors: a mechanistic study towards evaluating ionic leaching induced cellular inflammation |
| title_full_unstemmed | Bio-acceptability of wearable sensors: a mechanistic study towards evaluating ionic leaching induced cellular inflammation |
| title_short | Bio-acceptability of wearable sensors: a mechanistic study towards evaluating ionic leaching induced cellular inflammation |
| title_sort | bio acceptability of wearable sensors a mechanistic study towards evaluating ionic leaching induced cellular inflammation |
| url | https://doi.org/10.1038/s41598-022-13810-0 |
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