Transfer of the Dominant Virus Resistance Gene AV-1pro From Asparagus prostratus to Chromosome 2 of Garden Asparagus A. officinalis L.
An introgression breeding programme was carried out to transfer the virus resistance gene AV-1pro from the wild species Asparagus prostratus to the garden asparagus Asparagus officinalis. Serious crossing barriers caused by genetic distance and different ploidy levels of the crossing parents have be...
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| Format: | Article |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.809069/full |
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| author | Thomas Nothnagel Janine König Jens Keilwagen Eva-Maria Graner Jörg Plieske Holger Budahn |
| author_facet | Thomas Nothnagel Janine König Jens Keilwagen Eva-Maria Graner Jörg Plieske Holger Budahn |
| author_sort | Thomas Nothnagel |
| collection | DOAJ |
| description | An introgression breeding programme was carried out to transfer the virus resistance gene AV-1pro from the wild species Asparagus prostratus to the garden asparagus Asparagus officinalis. Serious crossing barriers caused by genetic distance and different ploidy levels of the crossing parents have been overcome using embryo rescue for the F1, BC1, and BC2 generations. The male and female fertility was widely restored in BC2 and was shown to be comparable to the cultivated asparagus. Five AV-1 resistant diploid (2n = 2x = 20) BC2 plants were selected and reciprocally backcrossed with asparagus cultivars. Segregation analyses of fourteen seedborne BC3 progenies suggested a monogenic dominant inheritance of the AV-1 resistance. Genotyping by sequencing analysis gave a strong hint for location of the resistance gene on asparagus Chromosome 2. Using an Axiom single nucleotide polymorphism (SNP) genotyping array for the analysis of three BC3 families with 10 AV-1 resistant and 10 AV-1 susceptible plants each, as well as 25 asparagus cultivars, the AV-1pro locus on Chromosome 2 was further narrowed down. The SNP with the highest LOD score was converted to a kompetitive allele specific PCR (KASP) marker, shown to be useful for the further backcross programme and serving as the starting point for the development of a diagnostic marker. |
| first_indexed | 2024-12-13T03:23:57Z |
| format | Article |
| id | doaj.art-e388a0e2345d4a8b9e5ab71fdf39b3da |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T03:23:57Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e388a0e2345d4a8b9e5ab71fdf39b3da2022-12-22T00:01:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-02-011210.3389/fpls.2021.809069809069Transfer of the Dominant Virus Resistance Gene AV-1pro From Asparagus prostratus to Chromosome 2 of Garden Asparagus A. officinalis L.Thomas Nothnagel0Janine König1Jens Keilwagen2Eva-Maria Graner3Jörg Plieske4Holger Budahn5Institute for Breeding Research on Horticultural Crops, Federal Research Centre for Cultivated Plants, Julius Kühn-Institut, Quedlinburg, GermanyInstitute for Breeding Research on Horticultural Crops, Federal Research Centre for Cultivated Plants, Julius Kühn-Institut, Quedlinburg, GermanyInstitute for Biosafety in Plant Biotechnology, Federal Research Centre for Cultivated Plants, Julius Kühn-Institut, Quedlinburg, GermanySGS INSTITUT FRESENIUS GmbH, TraitGenetics Section, Gatersleben, GermanySGS INSTITUT FRESENIUS GmbH, TraitGenetics Section, Gatersleben, GermanyInstitute for Breeding Research on Horticultural Crops, Federal Research Centre for Cultivated Plants, Julius Kühn-Institut, Quedlinburg, GermanyAn introgression breeding programme was carried out to transfer the virus resistance gene AV-1pro from the wild species Asparagus prostratus to the garden asparagus Asparagus officinalis. Serious crossing barriers caused by genetic distance and different ploidy levels of the crossing parents have been overcome using embryo rescue for the F1, BC1, and BC2 generations. The male and female fertility was widely restored in BC2 and was shown to be comparable to the cultivated asparagus. Five AV-1 resistant diploid (2n = 2x = 20) BC2 plants were selected and reciprocally backcrossed with asparagus cultivars. Segregation analyses of fourteen seedborne BC3 progenies suggested a monogenic dominant inheritance of the AV-1 resistance. Genotyping by sequencing analysis gave a strong hint for location of the resistance gene on asparagus Chromosome 2. Using an Axiom single nucleotide polymorphism (SNP) genotyping array for the analysis of three BC3 families with 10 AV-1 resistant and 10 AV-1 susceptible plants each, as well as 25 asparagus cultivars, the AV-1pro locus on Chromosome 2 was further narrowed down. The SNP with the highest LOD score was converted to a kompetitive allele specific PCR (KASP) marker, shown to be useful for the further backcross programme and serving as the starting point for the development of a diagnostic marker.https://www.frontiersin.org/articles/10.3389/fpls.2021.809069/fullAsparagus virus 1resistanceintrogressionSNP genotyping arrayGBSpollen |
| spellingShingle | Thomas Nothnagel Janine König Jens Keilwagen Eva-Maria Graner Jörg Plieske Holger Budahn Transfer of the Dominant Virus Resistance Gene AV-1pro From Asparagus prostratus to Chromosome 2 of Garden Asparagus A. officinalis L. Frontiers in Plant Science Asparagus virus 1 resistance introgression SNP genotyping array GBS pollen |
| title | Transfer of the Dominant Virus Resistance Gene AV-1pro From Asparagus prostratus to Chromosome 2 of Garden Asparagus A. officinalis L. |
| title_full | Transfer of the Dominant Virus Resistance Gene AV-1pro From Asparagus prostratus to Chromosome 2 of Garden Asparagus A. officinalis L. |
| title_fullStr | Transfer of the Dominant Virus Resistance Gene AV-1pro From Asparagus prostratus to Chromosome 2 of Garden Asparagus A. officinalis L. |
| title_full_unstemmed | Transfer of the Dominant Virus Resistance Gene AV-1pro From Asparagus prostratus to Chromosome 2 of Garden Asparagus A. officinalis L. |
| title_short | Transfer of the Dominant Virus Resistance Gene AV-1pro From Asparagus prostratus to Chromosome 2 of Garden Asparagus A. officinalis L. |
| title_sort | transfer of the dominant virus resistance gene av 1pro from asparagus prostratus to chromosome 2 of garden asparagus a officinalis l |
| topic | Asparagus virus 1 resistance introgression SNP genotyping array GBS pollen |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.809069/full |
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