Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (<i>Saccharum</i> spp.) under Drought Stress Conditions
The symbiotic associations between arbuscular mycorrhizal fungi (AMF) and plants can induce drought stress tolerance. In this study, we evaluated the effect of <i>Glomus intraradices</i>, a mycorrhizal fungus, on the ex vitro development and survival of sugarcane plantlets subjected to d...
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/3/687 |
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| author | José Luis Spinoso-Castillo María del Rosario Moreno-Hernández Eucario Mancilla-Álvarez Lino Sánchez-Segura Ricardo Sánchez-Páez Jericó Jabín Bello-Bello |
| author_facet | José Luis Spinoso-Castillo María del Rosario Moreno-Hernández Eucario Mancilla-Álvarez Lino Sánchez-Segura Ricardo Sánchez-Páez Jericó Jabín Bello-Bello |
| author_sort | José Luis Spinoso-Castillo |
| collection | DOAJ |
| description | The symbiotic associations between arbuscular mycorrhizal fungi (AMF) and plants can induce drought stress tolerance. In this study, we evaluated the effect of <i>Glomus intraradices</i>, a mycorrhizal fungus, on the ex vitro development and survival of sugarcane plantlets subjected to drought stress during the acclimatization stage of micropropagation. In vitro obtained sugarcane plantlets (<i>Saccharum</i> spp. cv Mex 69–290) were inoculated with different doses of <i>G. intraradices</i> (0, 100, and 200 spores per plantlet) during greenhouse acclimatization. Sixty days after inoculation, plantlets were temporarily subjected to drought stress. We evaluated the survival rate, total chlorophyll, total protein, carotenoids, proline, betaine glycine, soluble phenolic content, and antioxidant capacity every 3 days for 12 days. Symbiotic interaction was characterized by microscopy. Our results showed that the survival rate of inoculated plants was higher in 45% than the treatment without mycorrhizae. Total chlorophyll, protein, proline, betaine glycine content, and antioxidant capacity were increased in AMF inoculated plants. The soluble phenolic content was higher in non-inoculated plants than the treatment with mycorrhizae during the drought stress period. Microscopy showed the symbiotic relationship between plant and AMF. The early inoculation of 100 spores of <i>G. intraradices</i> per sugarcane plantlet during the acclimatization stage could represent a preconditioning advantage before transplanting into the field and establishing basic seedbeds. |
| first_indexed | 2024-03-11T09:29:52Z |
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| last_indexed | 2024-03-11T09:29:52Z |
| publishDate | 2023-02-01 |
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| series | Plants |
| spelling | doaj.art-e38bb5493c2e47269c44f2b8ed6cabc22023-11-16T17:45:59ZengMDPI AGPlants2223-77472023-02-0112368710.3390/plants12030687Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (<i>Saccharum</i> spp.) under Drought Stress ConditionsJosé Luis Spinoso-Castillo0María del Rosario Moreno-Hernández1Eucario Mancilla-Álvarez2Lino Sánchez-Segura3Ricardo Sánchez-Páez4Jericó Jabín Bello-Bello5Postgraduate College-Campus Cordoba, Km 348 Cordoba-Veracruz Federal Highway, Amatlan de los Reyes, Veracruz 94953, MexicoPostgraduate College-Campus Cordoba, Km 348 Cordoba-Veracruz Federal Highway, Amatlan de los Reyes, Veracruz 94953, MexicoPostgraduate College-Campus Cordoba, Km 348 Cordoba-Veracruz Federal Highway, Amatlan de los Reyes, Veracruz 94953, MexicoCINVESTAV, Department of Biotechnology and Biochemistry, Irapuato Unit, Km 9.6 North Beltway Highway Irapuato-Leon, Irapuato, Guanajuato 36821, MexicoPostgraduate College-Campus Cordoba, Km 348 Cordoba-Veracruz Federal Highway, Amatlan de los Reyes, Veracruz 94953, MexicoCONACYT-Postgraduate College-Campus Cordoba, Km 348 Cordoba-Veracruz Federal Highway, Amatlan de los Reyes, Veracruz 94953, MexicoThe symbiotic associations between arbuscular mycorrhizal fungi (AMF) and plants can induce drought stress tolerance. In this study, we evaluated the effect of <i>Glomus intraradices</i>, a mycorrhizal fungus, on the ex vitro development and survival of sugarcane plantlets subjected to drought stress during the acclimatization stage of micropropagation. In vitro obtained sugarcane plantlets (<i>Saccharum</i> spp. cv Mex 69–290) were inoculated with different doses of <i>G. intraradices</i> (0, 100, and 200 spores per plantlet) during greenhouse acclimatization. Sixty days after inoculation, plantlets were temporarily subjected to drought stress. We evaluated the survival rate, total chlorophyll, total protein, carotenoids, proline, betaine glycine, soluble phenolic content, and antioxidant capacity every 3 days for 12 days. Symbiotic interaction was characterized by microscopy. Our results showed that the survival rate of inoculated plants was higher in 45% than the treatment without mycorrhizae. Total chlorophyll, protein, proline, betaine glycine content, and antioxidant capacity were increased in AMF inoculated plants. The soluble phenolic content was higher in non-inoculated plants than the treatment with mycorrhizae during the drought stress period. Microscopy showed the symbiotic relationship between plant and AMF. The early inoculation of 100 spores of <i>G. intraradices</i> per sugarcane plantlet during the acclimatization stage could represent a preconditioning advantage before transplanting into the field and establishing basic seedbeds.https://www.mdpi.com/2223-7747/12/3/687<i>Glomus intraradices</i>chlorophyllcarotenoidsosmolytesantioxidant capacity |
| spellingShingle | José Luis Spinoso-Castillo María del Rosario Moreno-Hernández Eucario Mancilla-Álvarez Lino Sánchez-Segura Ricardo Sánchez-Páez Jericó Jabín Bello-Bello Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (<i>Saccharum</i> spp.) under Drought Stress Conditions Plants <i>Glomus intraradices</i> chlorophyll carotenoids osmolytes antioxidant capacity |
| title | Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (<i>Saccharum</i> spp.) under Drought Stress Conditions |
| title_full | Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (<i>Saccharum</i> spp.) under Drought Stress Conditions |
| title_fullStr | Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (<i>Saccharum</i> spp.) under Drought Stress Conditions |
| title_full_unstemmed | Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (<i>Saccharum</i> spp.) under Drought Stress Conditions |
| title_short | Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (<i>Saccharum</i> spp.) under Drought Stress Conditions |
| title_sort | arbuscular mycorrhizal symbiosis improves ex vitro acclimatization of sugarcane plantlets i saccharum i spp under drought stress conditions |
| topic | <i>Glomus intraradices</i> chlorophyll carotenoids osmolytes antioxidant capacity |
| url | https://www.mdpi.com/2223-7747/12/3/687 |
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