Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors
Abstract In this work, we present the development and biofunctionalization of a fiber-optic ball-resonator biosensor for the real-time detection of vaccinia poxvirus. We fabricated several ball-tip resonators, functionalized through a silanization process to immobilize two bioreceptors: the monoclon...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-44926-6 |
| _version_ | 1797452618631479296 |
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| author | Aida Rakhimbekova Baizak Kudaibergenov Kuanysh Seitkamal Aurora Bellone Ayazhan Dauletova Marzhan Sypabekova Massimo Olivero Guido Perrone Antonia Radaelli Carlo Zanotto Carlo De Giuli Morghen Luca Vangelista Daniele Tosi |
| author_facet | Aida Rakhimbekova Baizak Kudaibergenov Kuanysh Seitkamal Aurora Bellone Ayazhan Dauletova Marzhan Sypabekova Massimo Olivero Guido Perrone Antonia Radaelli Carlo Zanotto Carlo De Giuli Morghen Luca Vangelista Daniele Tosi |
| author_sort | Aida Rakhimbekova |
| collection | DOAJ |
| description | Abstract In this work, we present the development and biofunctionalization of a fiber-optic ball-resonator biosensor for the real-time detection of vaccinia poxvirus. We fabricated several ball-tip resonators, functionalized through a silanization process to immobilize two bioreceptors: the monoclonal anti-L1R antibody targeting the L1R protein, and the polyclonal rabbit serum antibodies targeting the whole vaccinia virus (VV) pathogen. Experimental measurements were carried out to detect VV in concentrations from 103 to 108 plaque-forming units (PFU), with a limit of detection of around 1.7–4.3 × 103 PFU and a log-quadratic pattern, with a response up to 5 × 10−4 RIU (refractive index units). The specificity was assessed against herpes simplex virus, used as a non-specific control, with the best results obtained with anti-L1R monoclonal antibodies, and through the detection of vaccinia virus/herpes simplex-1 combination. The obtained results provide a real-time viral recognition with a label-free sensing platform, having rapid response and ease of manufacturing, and paving the road to the seamless detection of poxviruses affecting different human and animal species using optical fibers. |
| first_indexed | 2024-03-09T15:11:14Z |
| format | Article |
| id | doaj.art-e38dc67c756a404cb302220ddbe19762 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:11:14Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e38dc67c756a404cb302220ddbe197622023-11-26T13:22:52ZengNature PortfolioScientific Reports2045-23222023-10-0113111110.1038/s41598-023-44926-6Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensorsAida Rakhimbekova0Baizak Kudaibergenov1Kuanysh Seitkamal2Aurora Bellone3Ayazhan Dauletova4Marzhan Sypabekova5Massimo Olivero6Guido Perrone7Antonia Radaelli8Carlo Zanotto9Carlo De Giuli Morghen10Luca Vangelista11Daniele Tosi12Department of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev UniversityDepartment of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev UniversityDepartment of Biomedical Sciences, School of Medicine, Nazarbayev UniversityDepartment of Electronics and Telecommunications, Politecnico Di TorinoDepartment of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev UniversityDepartment of Electrical & Computer Engineering, Baylor UniversityDepartment of Electronics and Telecommunications, Politecnico Di TorinoDepartment of Electronics and Telecommunications, Politecnico Di TorinoDepartment of Medical Biotechnologies and Translational Medicine, Laboratory of Molecular Virology and Recombinant Vaccine Development, University of MilanDepartment of Medical Biotechnologies and Translational Medicine, Laboratory of Molecular Virology and Recombinant Vaccine Development, University of MilanCatholic University “Our Lady of Good Counsel”Department of Biomedical Sciences, School of Medicine, Nazarbayev UniversityDepartment of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev UniversityAbstract In this work, we present the development and biofunctionalization of a fiber-optic ball-resonator biosensor for the real-time detection of vaccinia poxvirus. We fabricated several ball-tip resonators, functionalized through a silanization process to immobilize two bioreceptors: the monoclonal anti-L1R antibody targeting the L1R protein, and the polyclonal rabbit serum antibodies targeting the whole vaccinia virus (VV) pathogen. Experimental measurements were carried out to detect VV in concentrations from 103 to 108 plaque-forming units (PFU), with a limit of detection of around 1.7–4.3 × 103 PFU and a log-quadratic pattern, with a response up to 5 × 10−4 RIU (refractive index units). The specificity was assessed against herpes simplex virus, used as a non-specific control, with the best results obtained with anti-L1R monoclonal antibodies, and through the detection of vaccinia virus/herpes simplex-1 combination. The obtained results provide a real-time viral recognition with a label-free sensing platform, having rapid response and ease of manufacturing, and paving the road to the seamless detection of poxviruses affecting different human and animal species using optical fibers.https://doi.org/10.1038/s41598-023-44926-6 |
| spellingShingle | Aida Rakhimbekova Baizak Kudaibergenov Kuanysh Seitkamal Aurora Bellone Ayazhan Dauletova Marzhan Sypabekova Massimo Olivero Guido Perrone Antonia Radaelli Carlo Zanotto Carlo De Giuli Morghen Luca Vangelista Daniele Tosi Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors Scientific Reports |
| title | Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors |
| title_full | Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors |
| title_fullStr | Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors |
| title_full_unstemmed | Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors |
| title_short | Rapid detection of vaccinia virus using biofunctionalized fiber-optic ball-tip biosensors |
| title_sort | rapid detection of vaccinia virus using biofunctionalized fiber optic ball tip biosensors |
| url | https://doi.org/10.1038/s41598-023-44926-6 |
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