The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran
Background and Objectives: The emergence of extended-spectrum β-lactamase (ESBL)-producing Shigella spp. is of increasing clinical concern specially in children worldwide. The aim of this study was to investigate the occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Ira...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-06-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/628 |
| _version_ | 1819236385598472192 |
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| author | Reza Ranjbar Farzaneh-Mirsaeed Ghazi Shohreh Farshad Giovanni-Maurizio Giammanco Aurora Aleo Parviz Owlia Nematollah Jonaidi Nourkhoda Sadeghifard Caterina Mammina |
| author_facet | Reza Ranjbar Farzaneh-Mirsaeed Ghazi Shohreh Farshad Giovanni-Maurizio Giammanco Aurora Aleo Parviz Owlia Nematollah Jonaidi Nourkhoda Sadeghifard Caterina Mammina |
| author_sort | Reza Ranjbar |
| collection | DOAJ |
| description | Background and Objectives: The emergence of extended-spectrum β-lactamase (ESBL)-producing Shigella spp. is of increasing clinical concern specially in children worldwide. The aim of this study was to investigate the occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran.
Materials and Methods: The study included all Shigella isolates recovered from pediatric patients aged less than 12 years admitted to a major pediatric hospital in Tehran, Iran, from 2008 to 2010. Bacterial identification, antimicrobial susceptibility testing, extended spectrum β-lactamases (ESBLs) screening and confirmatory tests were performed according to the standard guidelines. Conjugal transfer experiments and plasmid analysis were also carried out. Polymerase chain reaction and sequencing were used to identify the genetic determinants responsible for ESBL production.
Results: Four out of 55 Shigella isolates, including three S. sonnei and one S. flexneri, showed an ESBL-positive phenotype. Plasmid transfer of the ESBL phenotype was successful for the S. flexneri isolate only. By PCR and sequencing, one S. sonnei isolate tested positive for the CMY-59 gene, while the other two S. sonnei and the S. flexneri isolates tested positive for the bla CTX-M-15 genes.
Conclusion: We found the prevalence of ESBL producing Shigella isolates was higher than detection rates observed in many other countries. Our finding raise concerns about the dissemination of ESBL among the strains of endemic S. sonnei throughout the country, because this species is now the most frequently isolated Shigella species in Iran and shigellosis by such strains in the community can pose a significant threat to patients and presents a challenge for disease management. |
| first_indexed | 2024-12-23T13:03:36Z |
| format | Article |
| id | doaj.art-e395c3eb02d4487eb29363abfd0aabb9 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-23T13:03:36Z |
| publishDate | 2013-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-e395c3eb02d4487eb29363abfd0aabb92022-12-21T17:45:57ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472013-06-0152The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, IranReza Ranjbar0Farzaneh-Mirsaeed Ghazi1Shohreh Farshad2Giovanni-Maurizio Giammanco3Aurora Aleo4Parviz Owlia5Nematollah Jonaidi6Nourkhoda Sadeghifard7Caterina Mammina8Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Department of Biochemistry, Payam Noor University, Tehran, Iran.Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Sciences for Health Promotion‘G. D’Alessandro’’, University, Palermo, Italy.Department of Sciences for Health Promotion‘G. D’Alessandro’’, University, Palermo, Italy.Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.Department of Sciences for Health Promotion‘G. D’Alessandro’’, University, Palermo, Italy.Background and Objectives: The emergence of extended-spectrum β-lactamase (ESBL)-producing Shigella spp. is of increasing clinical concern specially in children worldwide. The aim of this study was to investigate the occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran. Materials and Methods: The study included all Shigella isolates recovered from pediatric patients aged less than 12 years admitted to a major pediatric hospital in Tehran, Iran, from 2008 to 2010. Bacterial identification, antimicrobial susceptibility testing, extended spectrum β-lactamases (ESBLs) screening and confirmatory tests were performed according to the standard guidelines. Conjugal transfer experiments and plasmid analysis were also carried out. Polymerase chain reaction and sequencing were used to identify the genetic determinants responsible for ESBL production. Results: Four out of 55 Shigella isolates, including three S. sonnei and one S. flexneri, showed an ESBL-positive phenotype. Plasmid transfer of the ESBL phenotype was successful for the S. flexneri isolate only. By PCR and sequencing, one S. sonnei isolate tested positive for the CMY-59 gene, while the other two S. sonnei and the S. flexneri isolates tested positive for the bla CTX-M-15 genes. Conclusion: We found the prevalence of ESBL producing Shigella isolates was higher than detection rates observed in many other countries. Our finding raise concerns about the dissemination of ESBL among the strains of endemic S. sonnei throughout the country, because this species is now the most frequently isolated Shigella species in Iran and shigellosis by such strains in the community can pose a significant threat to patients and presents a challenge for disease management.https://ijm.tums.ac.ir/index.php/ijm/article/view/628Antibiotic resistanceESBLsShigella spp |
| spellingShingle | Reza Ranjbar Farzaneh-Mirsaeed Ghazi Shohreh Farshad Giovanni-Maurizio Giammanco Aurora Aleo Parviz Owlia Nematollah Jonaidi Nourkhoda Sadeghifard Caterina Mammina The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran Iranian Journal of Microbiology Antibiotic resistance ESBLs Shigella spp |
| title | The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran |
| title_full | The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran |
| title_fullStr | The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran |
| title_full_unstemmed | The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran |
| title_short | The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran |
| title_sort | occurrence of extended spectrum β lactamase producing shigella spp in tehran iran |
| topic | Antibiotic resistance ESBLs Shigella spp |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/628 |
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