Yeast strains from coconut toddy in Sri Lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materials
We previously isolated 18 Saccharomyces cerevisiae strains from coconut toddy in Sri Lanka. All the 18 strains (SLY-1 to SLY-18) could grow aerobically up to 18 mmol L−1 vanillin in yeast extract–peptone–dextrose (YPD) medium, and the SLY-10 strain showed the highest vanillin tolerance (up to 21 mmo...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2019.1676167 |
| _version_ | 1819110562005516288 |
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| author | Maduka Subodinee Ahangangoda Arachchige Osamu Mizutani Hirohide Toyama |
| author_facet | Maduka Subodinee Ahangangoda Arachchige Osamu Mizutani Hirohide Toyama |
| author_sort | Maduka Subodinee Ahangangoda Arachchige |
| collection | DOAJ |
| description | We previously isolated 18 Saccharomyces cerevisiae strains from coconut toddy in Sri Lanka. All the 18 strains (SLY-1 to SLY-18) could grow aerobically up to 18 mmol L−1 vanillin in yeast extract–peptone–dextrose (YPD) medium, and the SLY-10 strain showed the highest vanillin tolerance (up to 21 mmol L−1). Among these 18 strains, even in the presence of 24 mmol L−1 vanillin, five strains (SLY-3, SLY-4, SLY-8, SLY-9 and SLY-10) produced alcohol at 72 h in the range of 11.4–33.1 g L−1 and the SLY-10 strain showed highest alcohol production. The five strains showed the conversion of vanillin to vanillyl alcohol. They also tolerated other strong inhibitors: 4-hydroxybenzoic acid (24 mmol L−1), furfural (30 mmol L−1), 5-hydroxymethyl-2-furaldehyde (5-HMF, 36 mmol L−1) and acetic acid (75 mmol L−1). All these five strains showed no growth and no alcohol production when cultured in the medium with inhibitor mixture of 2.7 mmol L−1 vanillin, 30 mmol L−1 furfural, 30 mmol L−1 5-HMF, 75 mmol L−1 acetic acid and 75 mmol L−1 formic acid but showed significant growth and alcohol production of 16.2–32.7 g L−1 with inhibitor mixture of 30% concentration (0.81 mmol L−1 vanillin, 9 mmol L−1 furfural, 9 mmol L−1 5-HMF, 22.5 mmol L−1 acetic acid and 22.5 mmol L−1 formic acid). The four strains (SLY-3, SLY-4, SLY-8 and SLY-9) were shown to tolerate the inhibitor mixture to a similar extent and better than SLY-10, while SLY-10 was the best to vanillin alone. |
| first_indexed | 2024-12-22T03:43:41Z |
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| id | doaj.art-e397f0da775645599a5c365c1679b4ab |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-22T03:43:41Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-e397f0da775645599a5c365c1679b4ab2022-12-21T18:40:12ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-013311505151510.1080/13102818.2019.16761671676167Yeast strains from coconut toddy in Sri Lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materialsMaduka Subodinee Ahangangoda Arachchige0Osamu Mizutani1Hirohide Toyama2Kagoshima UniversityKagoshima UniversityKagoshima UniversityWe previously isolated 18 Saccharomyces cerevisiae strains from coconut toddy in Sri Lanka. All the 18 strains (SLY-1 to SLY-18) could grow aerobically up to 18 mmol L−1 vanillin in yeast extract–peptone–dextrose (YPD) medium, and the SLY-10 strain showed the highest vanillin tolerance (up to 21 mmol L−1). Among these 18 strains, even in the presence of 24 mmol L−1 vanillin, five strains (SLY-3, SLY-4, SLY-8, SLY-9 and SLY-10) produced alcohol at 72 h in the range of 11.4–33.1 g L−1 and the SLY-10 strain showed highest alcohol production. The five strains showed the conversion of vanillin to vanillyl alcohol. They also tolerated other strong inhibitors: 4-hydroxybenzoic acid (24 mmol L−1), furfural (30 mmol L−1), 5-hydroxymethyl-2-furaldehyde (5-HMF, 36 mmol L−1) and acetic acid (75 mmol L−1). All these five strains showed no growth and no alcohol production when cultured in the medium with inhibitor mixture of 2.7 mmol L−1 vanillin, 30 mmol L−1 furfural, 30 mmol L−1 5-HMF, 75 mmol L−1 acetic acid and 75 mmol L−1 formic acid but showed significant growth and alcohol production of 16.2–32.7 g L−1 with inhibitor mixture of 30% concentration (0.81 mmol L−1 vanillin, 9 mmol L−1 furfural, 9 mmol L−1 5-HMF, 22.5 mmol L−1 acetic acid and 22.5 mmol L−1 formic acid). The four strains (SLY-3, SLY-4, SLY-8 and SLY-9) were shown to tolerate the inhibitor mixture to a similar extent and better than SLY-10, while SLY-10 was the best to vanillin alone.http://dx.doi.org/10.1080/13102818.2019.1676167saccharomyces cerevisiaevanillinfermentation 5-hydroxymethyl-2- furaldehydetolerance |
| spellingShingle | Maduka Subodinee Ahangangoda Arachchige Osamu Mizutani Hirohide Toyama Yeast strains from coconut toddy in Sri Lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materials Biotechnology & Biotechnological Equipment saccharomyces cerevisiae vanillin fermentation 5-hydroxymethyl-2- furaldehyde tolerance |
| title | Yeast strains from coconut toddy in Sri Lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materials |
| title_full | Yeast strains from coconut toddy in Sri Lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materials |
| title_fullStr | Yeast strains from coconut toddy in Sri Lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materials |
| title_full_unstemmed | Yeast strains from coconut toddy in Sri Lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materials |
| title_short | Yeast strains from coconut toddy in Sri Lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materials |
| title_sort | yeast strains from coconut toddy in sri lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materials |
| topic | saccharomyces cerevisiae vanillin fermentation 5-hydroxymethyl-2- furaldehyde tolerance |
| url | http://dx.doi.org/10.1080/13102818.2019.1676167 |
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