Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis in <i>Lilium</i> ‘Siberia’
Light is an important environmental signal that governs plant growth, development, and metabolism. Constitutive photomorphogenic 1 (COP1) is a light signaling component that plays a vital role in plant light responses. We isolated the <i>COP1</i> gene (<i>LoCOP1</i>) from the...
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MDPI AG
2023-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/10/2004 |
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| author | Yang Liu Qin Wang Farhat Abbas Yiwei Zhou Jingjuan He Yanping Fan Rangcai Yu |
| author_facet | Yang Liu Qin Wang Farhat Abbas Yiwei Zhou Jingjuan He Yanping Fan Rangcai Yu |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Light is an important environmental signal that governs plant growth, development, and metabolism. Constitutive photomorphogenic 1 (COP1) is a light signaling component that plays a vital role in plant light responses. We isolated the <i>COP1</i> gene (<i>LoCOP1</i>) from the petals of <i>Lilium</i> ‘Siberia’ and investigated its function. The LoCOP1 protein was found to be the most similar to <i>Apostasia shenzhenica</i> COP1. LoCOP1 was found to be an important factor located in the nucleus and played a negative regulatory role in floral scent production and emission using the virus-induced gene silencing (VIGS) approach. The yeast two-hybrid, β-galactosidase, and bimolecular fluorescence complementation (BiFC) assays revealed that LoCOP1 interacts with LoMYB1 and LoMYB3. Furthermore, light modified both the subcellular distribution of LoCOP1 and its interactions with LoMYB1 and MYB3 in onion cells. The findings highlighted an important regulatory mechanism in the light signaling system that governs scent emission in <i>Lilium</i> ‘Siberia’ by the ubiquitination and degradation of transcription factors via the proteasome pathway. |
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| language | English |
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| spelling | doaj.art-e39afa0781a043b599c0ecb70997a3c12023-11-18T02:56:15ZengMDPI AGPlants2223-77472023-05-011210200410.3390/plants12102004Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis in <i>Lilium</i> ‘Siberia’Yang Liu0Qin Wang1Farhat Abbas2Yiwei Zhou3Jingjuan He4Yanping Fan5Rangcai Yu6The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Life Sciences, South China Agricultural University, Guangzhou 510642, ChinaLight is an important environmental signal that governs plant growth, development, and metabolism. Constitutive photomorphogenic 1 (COP1) is a light signaling component that plays a vital role in plant light responses. We isolated the <i>COP1</i> gene (<i>LoCOP1</i>) from the petals of <i>Lilium</i> ‘Siberia’ and investigated its function. The LoCOP1 protein was found to be the most similar to <i>Apostasia shenzhenica</i> COP1. LoCOP1 was found to be an important factor located in the nucleus and played a negative regulatory role in floral scent production and emission using the virus-induced gene silencing (VIGS) approach. The yeast two-hybrid, β-galactosidase, and bimolecular fluorescence complementation (BiFC) assays revealed that LoCOP1 interacts with LoMYB1 and LoMYB3. Furthermore, light modified both the subcellular distribution of LoCOP1 and its interactions with LoMYB1 and MYB3 in onion cells. The findings highlighted an important regulatory mechanism in the light signaling system that governs scent emission in <i>Lilium</i> ‘Siberia’ by the ubiquitination and degradation of transcription factors via the proteasome pathway.https://www.mdpi.com/2223-7747/12/10/2004<i>Lilium</i> ‘Siberia’floral scentlightLoCOP1LoMYBs |
| spellingShingle | Yang Liu Qin Wang Farhat Abbas Yiwei Zhou Jingjuan He Yanping Fan Rangcai Yu Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis in <i>Lilium</i> ‘Siberia’ Plants <i>Lilium</i> ‘Siberia’ floral scent light LoCOP1 LoMYBs |
| title | Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis in <i>Lilium</i> ‘Siberia’ |
| title_full | Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis in <i>Lilium</i> ‘Siberia’ |
| title_fullStr | Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis in <i>Lilium</i> ‘Siberia’ |
| title_full_unstemmed | Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis in <i>Lilium</i> ‘Siberia’ |
| title_short | Light Regulation of LoCOP1 and Its Role in Floral Scent Biosynthesis in <i>Lilium</i> ‘Siberia’ |
| title_sort | light regulation of locop1 and its role in floral scent biosynthesis in i lilium i siberia |
| topic | <i>Lilium</i> ‘Siberia’ floral scent light LoCOP1 LoMYBs |
| url | https://www.mdpi.com/2223-7747/12/10/2004 |
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