Isolation, Identification and Speciation of Enterococci by Conventional Method and their Antibiogram
Introduction: Enterococci are normal commensals in the gastrointestinal tract, oral cavity, vagina etc. They are organisms of low virulence, but are known to cause various clinical infections. Enterococcus is considered as an important nososcomial pathogen because of its intrinsic as well as ac...
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JCDR Research and Publications Pvt. Ltd.
2015-04-01
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| Online Access: | http://www.njlm.net/articles/PDF/2031/1-%2011684_F(P)_PF1(PAG)_PFA(P).pdf |
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| author | Shanmukhappa D Venkatesha Ajantha G S Amrutha Kumari B Mallikarjun Koppad |
| author_facet | Shanmukhappa D Venkatesha Ajantha G S Amrutha Kumari B Mallikarjun Koppad |
| author_sort | Shanmukhappa |
| collection | DOAJ |
| description | Introduction: Enterococci are normal commensals in
the gastrointestinal tract, oral cavity, vagina etc. They
are organisms of low virulence, but are known to cause
various clinical infections. Enterococcus is considered
as an important nososcomial pathogen because of its
intrinsic as well as acquired antibiotic resistance. The
increasing importance of Enterococcus is largely due
to their resistance to many antimicrobials particularly
intrinsically resistant Enterococci, which is the cause of
changing pattern of Enterococcal infection resulting in
treatment failures.
Objectives: 1. Isolation, Identification and speciation
of Enterococci from clinical specimens by conventional
method. 2. Determination of Antibiogram of such isolates
of Enterococci.
Materials and Methods: Over a period of one year,
3,197 various clinical specimens were processed
and a total of 80 strains of Enterococci isolated. Such
isolates were identified and speciated by recommended
conventional tests and biochemical reactions. Their
antibiotic susceptibility pattern was also determined to
the common antibiotics by disc diffusion method as per
the recommendations.
Results: Among the total 80 strains of Enterococci, 69
were E.faecalis, 10 were faecium and one was durans and
all the isolated strains were identifiable to the species
level by conventional method. Maximum numbers of
isolates were from urine specimen (38, 1.97%), followed
by exudates (32, 5.54%) and blood (10, 1.40%), and
with an overall incidence of isolation of Enterococci
from the above said specimens is 2.5%. Among 80
strains of Enterococci 35% were β hemolytic & 63.75%
isolates were non hemolytic. More than 50% of the total
isolates were resistant to Ampicillin (E.faecalis – 49.27%,
E.faecium -90%),Penicillin(E.faecalis–49.27%, E.faecium
-80%),Tetracycline(E.faecalis–57.97%, E.faecium -70%),
Erythromycin(E.faecalis –53.62% , E.faecium -60%) and
Gentamicin (E.faecalis –59.42% E.faecium -80%) and the
most useful antibiotic was ciprofloxacin to which 61.25%
(E.faecalis – 62.31%, E.faecium -50% ) of the total isolates
were sensitive.
Conclusion: From this study it was possible to identify all
the isolates of Enterococci to the species level by following
conventional methods. It was observed in our study that
there is more drug resistance to the tested antimicrobials
among E.faecalis isolates. |
| first_indexed | 2024-12-18T06:01:57Z |
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| issn | 2277-8551 2455-6882 |
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| series | National Journal of Laboratory Medicine |
| spelling | doaj.art-e39c4e17ec91441ba22ca24e4344a0152022-12-21T21:18:39ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822015-04-01421610.7860/NJLM/2015/11684:2031Isolation, Identification and Speciation of Enterococci by Conventional Method and their AntibiogramShanmukhappa0D Venkatesha1Ajantha G S 2Amrutha Kumari B 3Mallikarjun Koppad4Associate Professor, Department of Microbiology, Mandya Institute of Medical Sciences Mandya, India. Professor & HOD, Department of Microbiology, Mysore Medical College, Mysore, India. Professor, Department of Microbiology, SDMC, Dharwad, India. Associate Professor, Department of Microbiology, Mysore Medical College, Mysore, India.Assistant Professor, Department of Microbiology, Institute of Medical Sciences, Shimoga, India.Introduction: Enterococci are normal commensals in the gastrointestinal tract, oral cavity, vagina etc. They are organisms of low virulence, but are known to cause various clinical infections. Enterococcus is considered as an important nososcomial pathogen because of its intrinsic as well as acquired antibiotic resistance. The increasing importance of Enterococcus is largely due to their resistance to many antimicrobials particularly intrinsically resistant Enterococci, which is the cause of changing pattern of Enterococcal infection resulting in treatment failures. Objectives: 1. Isolation, Identification and speciation of Enterococci from clinical specimens by conventional method. 2. Determination of Antibiogram of such isolates of Enterococci. Materials and Methods: Over a period of one year, 3,197 various clinical specimens were processed and a total of 80 strains of Enterococci isolated. Such isolates were identified and speciated by recommended conventional tests and biochemical reactions. Their antibiotic susceptibility pattern was also determined to the common antibiotics by disc diffusion method as per the recommendations. Results: Among the total 80 strains of Enterococci, 69 were E.faecalis, 10 were faecium and one was durans and all the isolated strains were identifiable to the species level by conventional method. Maximum numbers of isolates were from urine specimen (38, 1.97%), followed by exudates (32, 5.54%) and blood (10, 1.40%), and with an overall incidence of isolation of Enterococci from the above said specimens is 2.5%. Among 80 strains of Enterococci 35% were β hemolytic & 63.75% isolates were non hemolytic. More than 50% of the total isolates were resistant to Ampicillin (E.faecalis – 49.27%, E.faecium -90%),Penicillin(E.faecalis–49.27%, E.faecium -80%),Tetracycline(E.faecalis–57.97%, E.faecium -70%), Erythromycin(E.faecalis –53.62% , E.faecium -60%) and Gentamicin (E.faecalis –59.42% E.faecium -80%) and the most useful antibiotic was ciprofloxacin to which 61.25% (E.faecalis – 62.31%, E.faecium -50% ) of the total isolates were sensitive. Conclusion: From this study it was possible to identify all the isolates of Enterococci to the species level by following conventional methods. It was observed in our study that there is more drug resistance to the tested antimicrobials among E.faecalis isolates.http://www.njlm.net/articles/PDF/2031/1-%2011684_F(P)_PF1(PAG)_PFA(P).pdfe.faecalise.faeciume.duranshaemolysisantibiogram |
| spellingShingle | Shanmukhappa D Venkatesha Ajantha G S Amrutha Kumari B Mallikarjun Koppad Isolation, Identification and Speciation of Enterococci by Conventional Method and their Antibiogram National Journal of Laboratory Medicine e.faecalis e.faecium e.durans haemolysis antibiogram |
| title | Isolation, Identification and Speciation of Enterococci by Conventional Method and their Antibiogram |
| title_full | Isolation, Identification and Speciation of Enterococci by Conventional Method and their Antibiogram |
| title_fullStr | Isolation, Identification and Speciation of Enterococci by Conventional Method and their Antibiogram |
| title_full_unstemmed | Isolation, Identification and Speciation of Enterococci by Conventional Method and their Antibiogram |
| title_short | Isolation, Identification and Speciation of Enterococci by Conventional Method and their Antibiogram |
| title_sort | isolation identification and speciation of enterococci by conventional method and their antibiogram |
| topic | e.faecalis e.faecium e.durans haemolysis antibiogram |
| url | http://www.njlm.net/articles/PDF/2031/1-%2011684_F(P)_PF1(PAG)_PFA(P).pdf |
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