Methods of obtaining multipotent stem cells of the placenta in rats
Placenta is a valuable source of multipotent stem cells (PMSC) widely used for cell therapy. Hence, the most optimal method of PMSC obtaining remains questionable. By methods of explant culturing and enzymatic digestion were obtained PMSC of fetal origin and multipotent features at 4 th passage. All...
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| Format: | Article |
| Language: | English |
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Taras Shevchenko National University of Kyiv
2016-07-01
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| Series: | Vìsnik: Kiïvsʹkij Nacìonalʹnij Unìversitet Imenì Tarasa Ševčenka. Bìologìâ |
| Subjects: | |
| Online Access: | http://biovestnik.com/index.php/biology/article/view/61 |
| _version_ | 1827842151468236800 |
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| author | G. Svitina L. Garmanchuk V. Shabliy |
| author_facet | G. Svitina L. Garmanchuk V. Shabliy |
| author_sort | G. Svitina |
| collection | DOAJ |
| description | Placenta is a valuable source of multipotent stem cells (PMSC) widely used for cell therapy. Hence, the most optimal method of PMSC obtaining remains questionable. By methods of explant culturing and enzymatic digestion were obtained PMSC of fetal origin and multipotent features at 4 th passage. Allogeneic rat serum is not favor PMSC establishment and growth, consequently the use is made of fetal bovine serum, that is xenogeneic for cell cultures. |
| first_indexed | 2024-03-12T08:04:21Z |
| format | Article |
| id | doaj.art-e3a0770e248e42849438eee94299c20c |
| institution | Directory Open Access Journal |
| issn | 1728-2748 2308-8036 |
| language | English |
| last_indexed | 2024-03-12T08:04:21Z |
| publishDate | 2016-07-01 |
| publisher | Taras Shevchenko National University of Kyiv |
| record_format | Article |
| series | Vìsnik: Kiïvsʹkij Nacìonalʹnij Unìversitet Imenì Tarasa Ševčenka. Bìologìâ |
| spelling | doaj.art-e3a0770e248e42849438eee94299c20c2023-09-02T19:40:14ZengTaras Shevchenko National University of KyivVìsnik: Kiïvsʹkij Nacìonalʹnij Unìversitet Imenì Tarasa Ševčenka. Bìologìâ1728-27482308-80362016-07-01702737710.17721/1728_2748.2015.70.73-7761Methods of obtaining multipotent stem cells of the placenta in ratsG. Svitina0L. Garmanchuk1V. Shabliy2Taras Shevchenko National University of Kyiv, KyivTaras Shevchenko National University of Kyiv, KyivInstitute of cell therapy, KyivPlacenta is a valuable source of multipotent stem cells (PMSC) widely used for cell therapy. Hence, the most optimal method of PMSC obtaining remains questionable. By methods of explant culturing and enzymatic digestion were obtained PMSC of fetal origin and multipotent features at 4 th passage. Allogeneic rat serum is not favor PMSC establishment and growth, consequently the use is made of fetal bovine serum, that is xenogeneic for cell cultures.http://biovestnik.com/index.php/biology/article/view/61Placenta, stem cells, cell therapy |
| spellingShingle | G. Svitina L. Garmanchuk V. Shabliy Methods of obtaining multipotent stem cells of the placenta in rats Vìsnik: Kiïvsʹkij Nacìonalʹnij Unìversitet Imenì Tarasa Ševčenka. Bìologìâ Placenta, stem cells, cell therapy |
| title | Methods of obtaining multipotent stem cells of the placenta in rats |
| title_full | Methods of obtaining multipotent stem cells of the placenta in rats |
| title_fullStr | Methods of obtaining multipotent stem cells of the placenta in rats |
| title_full_unstemmed | Methods of obtaining multipotent stem cells of the placenta in rats |
| title_short | Methods of obtaining multipotent stem cells of the placenta in rats |
| title_sort | methods of obtaining multipotent stem cells of the placenta in rats |
| topic | Placenta, stem cells, cell therapy |
| url | http://biovestnik.com/index.php/biology/article/view/61 |
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