VIOLATIONS OF CYTOKINE REGULATION IN THE PATHOGENESIS OF PROLIFERATIVE DIABETIC RETINOPATHY

Purpose. To study the content of the transforming growth factorbeta 2 (TGF-β2), vascular endothelial growth factor (VEGF), pigment epithelium factor (PEDF) and several cytokines in the vitreous body in patients with proliferative diabetic retinopathy (PDR), to evaluate an activity of proliferative a...

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Main Authors: D. V. Chernykh, E. V. Smirnov, O. M. Gorbenko, A. P. Shvayuk, O. O. Obuhova, L. A. Trunova, V. V. Chernykh, A. N. Trunov
Format: Article
Language:Russian
Published: Publishing house "Ophthalmology" 2015-10-01
Series:Офтальмохирургия
Subjects:
Online Access:https://www.ophthalmosurgery.ru/jour/article/view/94
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author D. V. Chernykh
E. V. Smirnov
O. M. Gorbenko
A. P. Shvayuk
O. O. Obuhova
L. A. Trunova
V. V. Chernykh
A. N. Trunov
author_facet D. V. Chernykh
E. V. Smirnov
O. M. Gorbenko
A. P. Shvayuk
O. O. Obuhova
L. A. Trunova
V. V. Chernykh
A. N. Trunov
author_sort D. V. Chernykh
collection DOAJ
description Purpose. To study the content of the transforming growth factorbeta 2 (TGF-β2), vascular endothelial growth factor (VEGF), pigment epithelium factor (PEDF) and several cytokines in the vitreous body in patients with proliferative diabetic retinopathy (PDR), to evaluate an activity of proliferative and immune-inflammatory process.Material and methods. Vitreous samples of patients with proliferative diabetic retinopathy and retinal detachment traction were examined as well as patients with traction retinal detachment without the PDR (control). Concentration of TGF- β 2, VEGF, PEDF MCP-1, interleukin 4, 6, 8, 10, 17A was determined by the ELISA method.Results. A reliable increase in the concentrations of pro-inflammatory cytokines IL-17A, IL-8, IL-6, MCP-1, anti-inflammatory cytokine IL-4, transforming growth factor-beta 2, vascular endothelial growth factor, factor of pigment epithelium and monocyte chematacticprotein-1 was detected in the vitreous of PDR patients.Conclusions. The findings indicate the existence of cytokine regulation violations in the PDR pathogenesis. It was noted that the activity of local inflammatory and proliferative processes was determined in the PDR pathogenesis. The revealed correlative relationships indicate interdependence of these processes. The detected reliable increase of monocyte chemotactic protein-1 in the vitreous of PDR patients, allows to make an assumption about its participation in the mechanisms of vascular proliferation.
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spelling doaj.art-e3a2b7f624974d879264d7a53fd195a92023-01-20T12:34:50ZrusPublishing house "Ophthalmology"Офтальмохирургия0235-41602312-49702015-10-0102505492VIOLATIONS OF CYTOKINE REGULATION IN THE PATHOGENESIS OF PROLIFERATIVE DIABETIC RETINOPATHYD. V. Chernykh0E. V. Smirnov1O. M. Gorbenko2A. P. Shvayuk3O. O. Obuhova4L. A. Trunova5V. V. Chernykh6A. N. Trunov7The S. Fyodorov Eye Microsurgery Federal State Institution, the Novosibirsk BranchThe S. Fyodorov Eye Microsurgery Federal State Institution, the Novosibirsk BranchThe Research Institute of Experimental and Clinical Medicine, NovosibirskThe Research Institute of Experimental and Clinical Medicine, NovosibirskThe Research Institute of Experimental and Clinical Medicine, NovosibirskThe Research Institute of Experimental and Clinical Medicine, NovosibirskThe S. Fyodorov Eye Microsurgery Federal State Institution, the Novosibirsk BranchThe S. Fyodorov Eye Microsurgery Federal State Institution, the Novosibirsk Branch The Research Institute of Experimental and Clinical Medicine, NovosibirskPurpose. To study the content of the transforming growth factorbeta 2 (TGF-β2), vascular endothelial growth factor (VEGF), pigment epithelium factor (PEDF) and several cytokines in the vitreous body in patients with proliferative diabetic retinopathy (PDR), to evaluate an activity of proliferative and immune-inflammatory process.Material and methods. Vitreous samples of patients with proliferative diabetic retinopathy and retinal detachment traction were examined as well as patients with traction retinal detachment without the PDR (control). Concentration of TGF- β 2, VEGF, PEDF MCP-1, interleukin 4, 6, 8, 10, 17A was determined by the ELISA method.Results. A reliable increase in the concentrations of pro-inflammatory cytokines IL-17A, IL-8, IL-6, MCP-1, anti-inflammatory cytokine IL-4, transforming growth factor-beta 2, vascular endothelial growth factor, factor of pigment epithelium and monocyte chematacticprotein-1 was detected in the vitreous of PDR patients.Conclusions. The findings indicate the existence of cytokine regulation violations in the PDR pathogenesis. It was noted that the activity of local inflammatory and proliferative processes was determined in the PDR pathogenesis. The revealed correlative relationships indicate interdependence of these processes. The detected reliable increase of monocyte chemotactic protein-1 in the vitreous of PDR patients, allows to make an assumption about its participation in the mechanisms of vascular proliferation.https://www.ophthalmosurgery.ru/jour/article/view/94proliferative diabetic retinopathyinflammationproliferationcytokinesgrowth factors
spellingShingle D. V. Chernykh
E. V. Smirnov
O. M. Gorbenko
A. P. Shvayuk
O. O. Obuhova
L. A. Trunova
V. V. Chernykh
A. N. Trunov
VIOLATIONS OF CYTOKINE REGULATION IN THE PATHOGENESIS OF PROLIFERATIVE DIABETIC RETINOPATHY
Офтальмохирургия
proliferative diabetic retinopathy
inflammation
proliferation
cytokines
growth factors
title VIOLATIONS OF CYTOKINE REGULATION IN THE PATHOGENESIS OF PROLIFERATIVE DIABETIC RETINOPATHY
title_full VIOLATIONS OF CYTOKINE REGULATION IN THE PATHOGENESIS OF PROLIFERATIVE DIABETIC RETINOPATHY
title_fullStr VIOLATIONS OF CYTOKINE REGULATION IN THE PATHOGENESIS OF PROLIFERATIVE DIABETIC RETINOPATHY
title_full_unstemmed VIOLATIONS OF CYTOKINE REGULATION IN THE PATHOGENESIS OF PROLIFERATIVE DIABETIC RETINOPATHY
title_short VIOLATIONS OF CYTOKINE REGULATION IN THE PATHOGENESIS OF PROLIFERATIVE DIABETIC RETINOPATHY
title_sort violations of cytokine regulation in the pathogenesis of proliferative diabetic retinopathy
topic proliferative diabetic retinopathy
inflammation
proliferation
cytokines
growth factors
url https://www.ophthalmosurgery.ru/jour/article/view/94
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AT evsmirnov violationsofcytokineregulationinthepathogenesisofproliferativediabeticretinopathy
AT omgorbenko violationsofcytokineregulationinthepathogenesisofproliferativediabeticretinopathy
AT apshvayuk violationsofcytokineregulationinthepathogenesisofproliferativediabeticretinopathy
AT ooobuhova violationsofcytokineregulationinthepathogenesisofproliferativediabeticretinopathy
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