Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping
The spike protein (S) plays a crucial role in porcine epidemic diarrhea virus (PEDV) infection and induces neutralizing antibodies. Mutations of the S protein are supposed to provide the main antigenic shift leading to the antigenic escape of PEDVs. It is therefore a significant question how much ac...
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MDPI AG
2022-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/7/1371 |
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| author | Ruisong Yu Shijuan Dong Bingqing Chen Yingjie Liu Fengping Li Fusheng Si Chunfang Xie Zhen Li |
| author_facet | Ruisong Yu Shijuan Dong Bingqing Chen Yingjie Liu Fengping Li Fusheng Si Chunfang Xie Zhen Li |
| author_sort | Ruisong Yu |
| collection | DOAJ |
| description | The spike protein (S) plays a crucial role in porcine epidemic diarrhea virus (PEDV) infection and induces neutralizing antibodies. Mutations of the S protein are supposed to provide the main antigenic shift leading to the antigenic escape of PEDVs. It is therefore a significant question how much accumulation of antigenic shift could lead to the antigenic escape of the variant PEDV. To provide an answer in the study, B cell epitopes (BCEs) on the S protein of the PEDV vaccine strain CV777 (S<sup>CV777</sup>) and variant strain SD2014 (S<sup>SD2014</sup>) were mapped using biosynthetic peptides and rabbit anti-PEDV S serum. Seventy-nine and 68 linear BCEs were identified from S<sup>CV777</sup> and S<sup>SD2014</sup>, respectively. While 66.2% of the BCEs of S<sup>SD2014</sup> could be recognized by anti-S<sup>CV777</sup> serum and 67.1% of S<sup>CV777</sup> BCEs could be recognized by anti-S<sup>SD2014</sup> serum, more than 40% of the BCEs identified using anti-S<sup>CV777</sup> serum on S<sup>CV777</sup> could not be recognized by anti-S<sup>SD2014</sup> serum and vice versa. The completely shared BCEs took low percentages of 29.4% and 25.3% for S<sup>SD2014</sup> and S<sup>CV777</sup>, respectively. These results indicate a low conservation of antigenicity of the S protein compared to a relatively high amino acid sequence similarity of 92.2% between the two strains. The study provided a BCE shift reference of PEDV antigenic escape and surveillance control. |
| first_indexed | 2024-03-09T12:52:25Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:52:25Z |
| publishDate | 2022-06-01 |
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| series | Viruses |
| spelling | doaj.art-e3a3cd85272a4c5a9e065668155d37712023-11-30T22:04:14ZengMDPI AGViruses1999-49152022-06-01147137110.3390/v14071371Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope MappingRuisong Yu0Shijuan Dong1Bingqing Chen2Yingjie Liu3Fengping Li4Fusheng Si5Chunfang Xie6Zhen Li7Institute of Animal Husbandry and Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Engineering Research Center of Breeding Pig, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, ChinaThe spike protein (S) plays a crucial role in porcine epidemic diarrhea virus (PEDV) infection and induces neutralizing antibodies. Mutations of the S protein are supposed to provide the main antigenic shift leading to the antigenic escape of PEDVs. It is therefore a significant question how much accumulation of antigenic shift could lead to the antigenic escape of the variant PEDV. To provide an answer in the study, B cell epitopes (BCEs) on the S protein of the PEDV vaccine strain CV777 (S<sup>CV777</sup>) and variant strain SD2014 (S<sup>SD2014</sup>) were mapped using biosynthetic peptides and rabbit anti-PEDV S serum. Seventy-nine and 68 linear BCEs were identified from S<sup>CV777</sup> and S<sup>SD2014</sup>, respectively. While 66.2% of the BCEs of S<sup>SD2014</sup> could be recognized by anti-S<sup>CV777</sup> serum and 67.1% of S<sup>CV777</sup> BCEs could be recognized by anti-S<sup>SD2014</sup> serum, more than 40% of the BCEs identified using anti-S<sup>CV777</sup> serum on S<sup>CV777</sup> could not be recognized by anti-S<sup>SD2014</sup> serum and vice versa. The completely shared BCEs took low percentages of 29.4% and 25.3% for S<sup>SD2014</sup> and S<sup>CV777</sup>, respectively. These results indicate a low conservation of antigenicity of the S protein compared to a relatively high amino acid sequence similarity of 92.2% between the two strains. The study provided a BCE shift reference of PEDV antigenic escape and surveillance control.https://www.mdpi.com/1999-4915/14/7/1371porcine epidemic diarrhea virusspike proteinepitope mappingantigenic alteration |
| spellingShingle | Ruisong Yu Shijuan Dong Bingqing Chen Yingjie Liu Fengping Li Fusheng Si Chunfang Xie Zhen Li Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping Viruses porcine epidemic diarrhea virus spike protein epitope mapping antigenic alteration |
| title | Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping |
| title_full | Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping |
| title_fullStr | Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping |
| title_full_unstemmed | Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping |
| title_short | Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping |
| title_sort | antigenicity alternations of variant pedv s protein disclosed by linear b cell epitope mapping |
| topic | porcine epidemic diarrhea virus spike protein epitope mapping antigenic alteration |
| url | https://www.mdpi.com/1999-4915/14/7/1371 |
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