Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene
The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-04-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506119300509 |
| _version_ | 1818264106868670464 |
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| author | Samuel McLenachan Elaine Y.M. Wong Xiao Zhang Fiona Leith Sang Yoon Moon Dan Zhang Shang-Chih Chen Jennifer A. Thompson Terri McLaren Tina Lamey John N. De Roach Marcus D. Atlas Rodney J. Dilley Fred K. Chen |
| author_facet | Samuel McLenachan Elaine Y.M. Wong Xiao Zhang Fiona Leith Sang Yoon Moon Dan Zhang Shang-Chih Chen Jennifer A. Thompson Terri McLaren Tina Lamey John N. De Roach Marcus D. Atlas Rodney J. Dilley Fred K. Chen |
| author_sort | Samuel McLenachan |
| collection | DOAJ |
| description | The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages. |
| first_indexed | 2024-12-12T19:29:39Z |
| format | Article |
| id | doaj.art-e3a7a0cbbc7c4e79aaca9a594d7dad71 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-12T19:29:39Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-e3a7a0cbbc7c4e79aaca9a594d7dad712022-12-22T00:14:26ZengElsevierStem Cell Research1873-50612019-04-0136Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A geneSamuel McLenachan0Elaine Y.M. Wong1Xiao Zhang2Fiona Leith3Sang Yoon Moon4Dan Zhang5Shang-Chih Chen6Jennifer A. Thompson7Terri McLaren8Tina Lamey9John N. De Roach10Marcus D. Atlas11Rodney J. Dilley12Fred K. Chen13Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Lions Eye Institute Australia, Nedlands, Western Australia, AustraliaEar Science Institute Australia, Nedlands, Western Australia, Australia; School of Pharmacy and Biomedical Sciences, Faculty of Health Sciences, Curtin University, Bentley, Western Australia, Australia; Centre for Neurological & Neuromuscular Diseases, The University of Western Australia, Crawley, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Lions Eye Institute Australia, Nedlands, Western Australia, AustraliaEar Science Institute Australia, Nedlands, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, AustraliaLions Eye Institute Australia, Nedlands, Western Australia, AustraliaLions Eye Institute Australia, Nedlands, Western Australia, AustraliaAustralian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Perth, Western Australia, AustraliaEar Science Institute Australia, Nedlands, Western Australia, Australia; Ear Sciences Centre, The University of Western Australia, Nedlands, Western Australia, AustraliaEar Science Institute Australia, Nedlands, Western Australia, Australia; Centre for Cell Therapy and Regenerative Medicine, The University of Western Australia, Australia; Ear Sciences Centre, The University of Western Australia, Nedlands, Western Australia, AustraliaCentre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Lions Eye Institute Australia, Nedlands, Western Australia, Australia; Department of Ophthalmology, Royal Perth Hospital, Perth, Western Australia, Australia; Corresponding author at: Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia.The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.http://www.sciencedirect.com/science/article/pii/S1873506119300509 |
| spellingShingle | Samuel McLenachan Elaine Y.M. Wong Xiao Zhang Fiona Leith Sang Yoon Moon Dan Zhang Shang-Chih Chen Jennifer A. Thompson Terri McLaren Tina Lamey John N. De Roach Marcus D. Atlas Rodney J. Dilley Fred K. Chen Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene Stem Cell Research |
| title | Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene |
| title_full | Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene |
| title_fullStr | Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene |
| title_full_unstemmed | Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene |
| title_short | Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene |
| title_sort | generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the ush2a gene |
| url | http://www.sciencedirect.com/science/article/pii/S1873506119300509 |
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