Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species
Background:Lung cancer has a very high incidence rate and is one of the commonly diagnosed tumors in developed countries.Aims:To investigate the effect of wogonin on A549 and A427 lung cancer cells and explore the mechanism involved.Study Design:Cell study.Methods:The cytotoxicity effect of wogonin...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2020-01-01
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| Series: | Balkan Medical Journal |
| Subjects: | |
| Online Access: |
http://balkanmedicaljournal.org/text.php?lang=en&id=2142
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| Summary: | Background:Lung cancer has a very high incidence rate and is one of the commonly diagnosed tumors in developed countries.Aims:To investigate the effect of wogonin on A549 and A427 lung cancer cells and explore the mechanism involved.Study Design:Cell study.Methods:The cytotoxicity effect of wogonin on A549 and A427 lung cancer and BEAS-2B cells was assessed by MTT assay. The onset of apoptosis was assessed by flow cytometry using Annexin V FITC/PI staining. Western blotting was used for the determination of changes in apoptotic protein expression.Results:Wogonin treatment exhibited cytotoxicity effect selectively on A549 and A427 cells without affecting BEAS-2B normal lung cells. The viability of A549 and A427 cells was reduced to 31% and 34%, respectively, on treatment with 50 μM of wogonin; however, there was no significant reduction in BEAS-2B cell viability on treatment with the same concentration of it. Moreover, the percentage of apoptotic A427 cells showed a significant (p<0.049) increase on treatment with wogonin. Furthermore, the treatment led to a marked increase in the activation of caspase 3/8/9 and the generation of reactive oxygen species (ROS) at 72 h in A427 cells. Digital tomosynthesis studies showed a marked reduction in tumor development on treatment with wogonin.Conclusion:Wogonin treatment specifically exhibits a cytotoxic effect on lung cancer cells and this effect is associated with activation of apoptosis and generation of reactive oxygen species. |
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| ISSN: | 2146-3123 2146-3131 |