Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species
Background:Lung cancer has a very high incidence rate and is one of the commonly diagnosed tumors in developed countries.Aims:To investigate the effect of wogonin on A549 and A427 lung cancer cells and explore the mechanism involved.Study Design:Cell study.Methods:The cytotoxicity effect of wogonin...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2020-01-01
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| Series: | Balkan Medical Journal |
| Subjects: | |
| Online Access: |
http://balkanmedicaljournal.org/text.php?lang=en&id=2142
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| _version_ | 1797916116203339776 |
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| author | Chengyang Wang Chuangcheng Cui |
| author_facet | Chengyang Wang Chuangcheng Cui |
| author_sort | Chengyang Wang |
| collection | DOAJ |
| description | Background:Lung cancer has a very high incidence rate and is one of the commonly diagnosed tumors in developed countries.Aims:To investigate the effect of wogonin on A549 and A427 lung cancer cells and explore the mechanism involved.Study Design:Cell study.Methods:The cytotoxicity effect of wogonin on A549 and A427 lung cancer and BEAS-2B cells was assessed by MTT assay. The onset of apoptosis was assessed by flow cytometry using Annexin V FITC/PI staining. Western blotting was used for the determination of changes in apoptotic protein expression.Results:Wogonin treatment exhibited cytotoxicity effect selectively on A549 and A427 cells without affecting BEAS-2B normal lung cells. The viability of A549 and A427 cells was reduced to 31% and 34%, respectively, on treatment with 50 μM of wogonin; however, there was no significant reduction in BEAS-2B cell viability on treatment with the same concentration of it. Moreover, the percentage of apoptotic A427 cells showed a significant (p<0.049) increase on treatment with wogonin. Furthermore, the treatment led to a marked increase in the activation of caspase 3/8/9 and the generation of reactive oxygen species (ROS) at 72 h in A427 cells. Digital tomosynthesis studies showed a marked reduction in tumor development on treatment with wogonin.Conclusion:Wogonin treatment specifically exhibits a cytotoxic effect on lung cancer cells and this effect is associated with activation of apoptosis and generation of reactive oxygen species. |
| first_indexed | 2024-04-10T12:52:07Z |
| format | Article |
| id | doaj.art-e3a96dba29934974b56b97f1003ee1b3 |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T12:52:07Z |
| publishDate | 2020-01-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-e3a96dba29934974b56b97f1003ee1b32023-02-15T16:13:42ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312020-01-01371293310.4274/balkanmedj.galenos.2019.2019.7.7513049054Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen SpeciesChengyang Wang0Chuangcheng Cui1 Department of Radiology, The Ninth Hospital of Xi’an Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China Department of Radiology, The Ninth Hospital of Xi’an Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China Background:Lung cancer has a very high incidence rate and is one of the commonly diagnosed tumors in developed countries.Aims:To investigate the effect of wogonin on A549 and A427 lung cancer cells and explore the mechanism involved.Study Design:Cell study.Methods:The cytotoxicity effect of wogonin on A549 and A427 lung cancer and BEAS-2B cells was assessed by MTT assay. The onset of apoptosis was assessed by flow cytometry using Annexin V FITC/PI staining. Western blotting was used for the determination of changes in apoptotic protein expression.Results:Wogonin treatment exhibited cytotoxicity effect selectively on A549 and A427 cells without affecting BEAS-2B normal lung cells. The viability of A549 and A427 cells was reduced to 31% and 34%, respectively, on treatment with 50 μM of wogonin; however, there was no significant reduction in BEAS-2B cell viability on treatment with the same concentration of it. Moreover, the percentage of apoptotic A427 cells showed a significant (p<0.049) increase on treatment with wogonin. Furthermore, the treatment led to a marked increase in the activation of caspase 3/8/9 and the generation of reactive oxygen species (ROS) at 72 h in A427 cells. Digital tomosynthesis studies showed a marked reduction in tumor development on treatment with wogonin.Conclusion:Wogonin treatment specifically exhibits a cytotoxic effect on lung cancer cells and this effect is associated with activation of apoptosis and generation of reactive oxygen species. http://balkanmedicaljournal.org/text.php?lang=en&id=2142 apoptosiscaspasescell studycytotoxicitylung cancerwagonin |
| spellingShingle | Chengyang Wang Chuangcheng Cui Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species Balkan Medical Journal apoptosis caspases cell study cytotoxicity lung cancer wagonin |
| title | Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species |
| title_full | Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species |
| title_fullStr | Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species |
| title_full_unstemmed | Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species |
| title_short | Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species |
| title_sort | inhibition of lung cancer proliferation by wogonin is associated with activation of apoptosis and generation of reactive oxygen species |
| topic | apoptosis caspases cell study cytotoxicity lung cancer wagonin |
| url |
http://balkanmedicaljournal.org/text.php?lang=en&id=2142
|
| work_keys_str_mv | AT chengyangwang inhibitionoflungcancerproliferationbywogoninisassociatedwithactivationofapoptosisandgenerationofreactiveoxygenspecies AT chuangchengcui inhibitionoflungcancerproliferationbywogoninisassociatedwithactivationofapoptosisandgenerationofreactiveoxygenspecies |