DNA methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell‐rich histological mimics
Abstract Sporadic giant cell granulomas (GCGs) of the jaws and cherubism‐associated giant cell lesions share histopathological features and microscopic diagnosis alone can be challenging. Additionally, GCG can morphologically closely resemble other giant cell‐rich lesions, including non‐ossifying fi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-11-01
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| Series: | The Journal of Pathology: Clinical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cjp2.337 |
| _version_ | 1797665440371048448 |
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| author | Letícia Martins Guimarães Daniel Baumhoer Vanghelita Andrei Dennis Friedel Christian Koelsche Ricardo Santiago Gomez Andreas von Deimling Carolina Cavalieri Gomes |
| author_facet | Letícia Martins Guimarães Daniel Baumhoer Vanghelita Andrei Dennis Friedel Christian Koelsche Ricardo Santiago Gomez Andreas von Deimling Carolina Cavalieri Gomes |
| author_sort | Letícia Martins Guimarães |
| collection | DOAJ |
| description | Abstract Sporadic giant cell granulomas (GCGs) of the jaws and cherubism‐associated giant cell lesions share histopathological features and microscopic diagnosis alone can be challenging. Additionally, GCG can morphologically closely resemble other giant cell‐rich lesions, including non‐ossifying fibroma (NOF), aneurysmal bone cyst (ABC), giant cell tumour of bone (GCTB), and chondroblastoma. The epigenetic basis of these giant cell‐rich tumours is unclear and DNA methylation profiling has been shown to be clinically useful for the diagnosis of other tumour types. Therefore, we aimed to assess the DNA methylation profile of central and peripheral sporadic GCG and cherubism to test whether DNA methylation patterns can help to distinguish them. Additionally, we compared the DNA methylation profile of these lesions with those of other giant cell‐rich mimics to investigate if the microscopic similarities extend to the epigenetic level. DNA methylation analysis was performed for central (n = 10) and peripheral (n = 10) GCG, cherubism (n = 6), NOF (n = 10), ABC (n = 16), GCTB (n = 9), and chondroblastoma (n = 10) using the Infinium Human Methylation EPIC Chip. Central and peripheral sporadic GCG and cherubism share a related DNA methylation pattern, with those of peripheral GCG and cherubism appearing slightly distinct, while central GCG shows overlap with both of the former. NOF, ABC, GCTB, and chondroblastoma, on the other hand, have distinct methylation patterns. The global and enhancer‐associated CpG DNA methylation values showed a similar distribution pattern among central and peripheral GCG and cherubism, with cherubism showing the lowest and peripheral GCG having the highest median values. By contrast, promoter regions showed a different methylation distribution pattern, with cherubism showing the highest median values. In conclusion, DNA methylation profiling is currently not capable of clearly distinguishing sporadic and cherubism‐associated giant cell lesions. Conversely, it could discriminate sporadic GCG of the jaws from their giant cell‐rich mimics (NOF, ABC, GCTB, and chondroblastoma). |
| first_indexed | 2024-03-11T19:43:58Z |
| format | Article |
| id | doaj.art-e3ab16ac9e2d48978958b240ef833eeb |
| institution | Directory Open Access Journal |
| issn | 2056-4538 |
| language | English |
| last_indexed | 2024-03-11T19:43:58Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Journal of Pathology: Clinical Research |
| spelling | doaj.art-e3ab16ac9e2d48978958b240ef833eeb2023-10-06T05:04:38ZengWileyThe Journal of Pathology: Clinical Research2056-45382023-11-019646447410.1002/cjp2.337DNA methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell‐rich histological mimicsLetícia Martins Guimarães0Daniel Baumhoer1Vanghelita Andrei2Dennis Friedel3Christian Koelsche4Ricardo Santiago Gomez5Andreas von Deimling6Carolina Cavalieri Gomes7Department of Pathology, Biological Science Institute Universidade Federal de Minas Gerais (UFMG) Belo Horizonte Minas Gerais BrazilBone Tumor Reference Centre, Institute of Pathology University Hospital Basel and University of Basel Basel SwitzerlandBone Tumor Reference Centre, Institute of Pathology University Hospital Basel and University of Basel Basel SwitzerlandDepartment of Neuropathology, Institute of Pathology Heidelberg University Hospital Heidelberg GermanyDepartment of Neuropathology, Institute of Pathology Heidelberg University Hospital Heidelberg GermanyDepartment of Oral Surgery and Pathology, School of Dentistry Universidade Federal de Minas Gerais (UFMG) Belo Horizonte Minas Gerais BrazilDepartment of Neuropathology, Institute of Pathology Heidelberg University Hospital Heidelberg GermanyDepartment of Pathology, Biological Science Institute Universidade Federal de Minas Gerais (UFMG) Belo Horizonte Minas Gerais BrazilAbstract Sporadic giant cell granulomas (GCGs) of the jaws and cherubism‐associated giant cell lesions share histopathological features and microscopic diagnosis alone can be challenging. Additionally, GCG can morphologically closely resemble other giant cell‐rich lesions, including non‐ossifying fibroma (NOF), aneurysmal bone cyst (ABC), giant cell tumour of bone (GCTB), and chondroblastoma. The epigenetic basis of these giant cell‐rich tumours is unclear and DNA methylation profiling has been shown to be clinically useful for the diagnosis of other tumour types. Therefore, we aimed to assess the DNA methylation profile of central and peripheral sporadic GCG and cherubism to test whether DNA methylation patterns can help to distinguish them. Additionally, we compared the DNA methylation profile of these lesions with those of other giant cell‐rich mimics to investigate if the microscopic similarities extend to the epigenetic level. DNA methylation analysis was performed for central (n = 10) and peripheral (n = 10) GCG, cherubism (n = 6), NOF (n = 10), ABC (n = 16), GCTB (n = 9), and chondroblastoma (n = 10) using the Infinium Human Methylation EPIC Chip. Central and peripheral sporadic GCG and cherubism share a related DNA methylation pattern, with those of peripheral GCG and cherubism appearing slightly distinct, while central GCG shows overlap with both of the former. NOF, ABC, GCTB, and chondroblastoma, on the other hand, have distinct methylation patterns. The global and enhancer‐associated CpG DNA methylation values showed a similar distribution pattern among central and peripheral GCG and cherubism, with cherubism showing the lowest and peripheral GCG having the highest median values. By contrast, promoter regions showed a different methylation distribution pattern, with cherubism showing the highest median values. In conclusion, DNA methylation profiling is currently not capable of clearly distinguishing sporadic and cherubism‐associated giant cell lesions. Conversely, it could discriminate sporadic GCG of the jaws from their giant cell‐rich mimics (NOF, ABC, GCTB, and chondroblastoma).https://doi.org/10.1002/cjp2.337epigeneticsDNA methylationcopy number analysisgiant cell granulomascherubismaneurysmal bone cyst |
| spellingShingle | Letícia Martins Guimarães Daniel Baumhoer Vanghelita Andrei Dennis Friedel Christian Koelsche Ricardo Santiago Gomez Andreas von Deimling Carolina Cavalieri Gomes DNA methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell‐rich histological mimics The Journal of Pathology: Clinical Research epigenetics DNA methylation copy number analysis giant cell granulomas cherubism aneurysmal bone cyst |
| title | DNA methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell‐rich histological mimics |
| title_full | DNA methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell‐rich histological mimics |
| title_fullStr | DNA methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell‐rich histological mimics |
| title_full_unstemmed | DNA methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell‐rich histological mimics |
| title_short | DNA methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell‐rich histological mimics |
| title_sort | dna methylation profile discriminates sporadic giant cell granulomas of the jaws and cherubism from their giant cell rich histological mimics |
| topic | epigenetics DNA methylation copy number analysis giant cell granulomas cherubism aneurysmal bone cyst |
| url | https://doi.org/10.1002/cjp2.337 |
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