Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage
The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen...
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MDPI AG
2020-09-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/9/9/300 |
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| author | Sara Sadeghi Raquel Del Gallego Balma García-Colomer Ernesto A. Gómez Jesús L. Yániz Jaime Gosálvez Carmen López-Fernández Miguel A. Silvestre |
| author_facet | Sara Sadeghi Raquel Del Gallego Balma García-Colomer Ernesto A. Gómez Jesús L. Yániz Jaime Gosálvez Carmen López-Fernández Miguel A. Silvestre |
| author_sort | Sara Sadeghi |
| collection | DOAJ |
| description | The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 °C or 17 °C), durations (0, 24 and 48 h) and sperm concentrations (250 × 10<sup>6</sup> sperm/mL (1:2 dilution rate), 166.7 × 10<sup>6</sup> sperm/mL (1:3 dilution rate) or 50 × 10<sup>6</sup> sperm/mL (1:10 dilution rate)) on sperm motility and kinetics were studied. In the second experiment, the effect of temperature, sperm washing and concentration on sperm motility and DNA fragmentation was studied. Finally, the effect of sperm concentration and duration of storage at 5 °C on sperm motility, response to oxidative stress and MMP was examined. We found that refrigerated liquid storage of goat sperm impaired sperm quality, such as motility, MMP and response to oxidation, as storage time increased; however, sperm DNA fragmentation index was not significantly affected. Liquid storage at 5 °C preserved higher total motility than at 17 °C. Moreover, we observed that the reduction of sperm concentration below 500 × 10<sup>6</sup> sperm/mL did not seem to improve the quality of spermatozoa conserved in milk-based extender in the conditions tested. |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T16:13:06Z |
| publishDate | 2020-09-01 |
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| series | Biology |
| spelling | doaj.art-e3ae0d24afab4a3caa51fbe2488b33a12023-11-20T14:20:20ZengMDPI AGBiology2079-77372020-09-019930010.3390/biology9090300Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid StorageSara Sadeghi0Raquel Del Gallego1Balma García-Colomer2Ernesto A. Gómez3Jesús L. Yániz4Jaime Gosálvez5Carmen López-Fernández6Miguel A. Silvestre7Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, 46100 Valencia, SpainDepartamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, 46100 Valencia, SpainDepartamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, 46100 Valencia, SpainCentro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias, Pol. La Esperanza 100, 12400 Castellón, SpainBIOFITER Research Group, Institute of Environmental Sciences (IUCA), University of Zaragoza, 22071 Huesca, SpainDepartment of Biology, Universidad Autónoma de Madrid, 28049 Madrid, SpainDepartment of Biology, Universidad Autónoma de Madrid, 28049 Madrid, SpainDepartamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, 46100 Valencia, SpainThe use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 °C or 17 °C), durations (0, 24 and 48 h) and sperm concentrations (250 × 10<sup>6</sup> sperm/mL (1:2 dilution rate), 166.7 × 10<sup>6</sup> sperm/mL (1:3 dilution rate) or 50 × 10<sup>6</sup> sperm/mL (1:10 dilution rate)) on sperm motility and kinetics were studied. In the second experiment, the effect of temperature, sperm washing and concentration on sperm motility and DNA fragmentation was studied. Finally, the effect of sperm concentration and duration of storage at 5 °C on sperm motility, response to oxidative stress and MMP was examined. We found that refrigerated liquid storage of goat sperm impaired sperm quality, such as motility, MMP and response to oxidation, as storage time increased; however, sperm DNA fragmentation index was not significantly affected. Liquid storage at 5 °C preserved higher total motility than at 17 °C. Moreover, we observed that the reduction of sperm concentration below 500 × 10<sup>6</sup> sperm/mL did not seem to improve the quality of spermatozoa conserved in milk-based extender in the conditions tested.https://www.mdpi.com/2079-7737/9/9/300goat spermliquid storagemotilityDNA fragmentationmitochondrial activity |
| spellingShingle | Sara Sadeghi Raquel Del Gallego Balma García-Colomer Ernesto A. Gómez Jesús L. Yániz Jaime Gosálvez Carmen López-Fernández Miguel A. Silvestre Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage Biology goat sperm liquid storage motility DNA fragmentation mitochondrial activity |
| title | Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage |
| title_full | Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage |
| title_fullStr | Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage |
| title_full_unstemmed | Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage |
| title_short | Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage |
| title_sort | effect of sperm concentration and storage temperature on goat spermatozoa during liquid storage |
| topic | goat sperm liquid storage motility DNA fragmentation mitochondrial activity |
| url | https://www.mdpi.com/2079-7737/9/9/300 |
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