Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modeling
ObjectiveThis study aimed to investigate the feasibility of Transcranial Doppler Ultrasonography (TCD) in evaluating neonatal hypoxic-ischemic encephalopathy (NHIE) modeling through monitoring the alteration of cerebrovascular flow in neonatal hypoxic-ischemic (HI) rats.MethodsPostnatal 7-day-old Sp...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2023.962001/full |
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| author | Jin-Xiang Liu Jin-Xiang Liu Chang-Le Fang Chang-Le Fang Kun Zhang Rui-Fang Ma Hong-Su Zhou Li Chen Qiu-Lin Wang Yu-Xuan Lu Ting-Hua Wang Ting-Hua Wang Liu-Lin Xiong Liu-Lin Xiong |
| author_facet | Jin-Xiang Liu Jin-Xiang Liu Chang-Le Fang Chang-Le Fang Kun Zhang Rui-Fang Ma Hong-Su Zhou Li Chen Qiu-Lin Wang Yu-Xuan Lu Ting-Hua Wang Ting-Hua Wang Liu-Lin Xiong Liu-Lin Xiong |
| author_sort | Jin-Xiang Liu |
| collection | DOAJ |
| description | ObjectiveThis study aimed to investigate the feasibility of Transcranial Doppler Ultrasonography (TCD) in evaluating neonatal hypoxic-ischemic encephalopathy (NHIE) modeling through monitoring the alteration of cerebrovascular flow in neonatal hypoxic-ischemic (HI) rats.MethodsPostnatal 7-day-old Sprague Dawley (SD) rats were divided into the control group, HI group, and hypoxia (H) group. TCD was applied to assess the changes of cerebral blood vessels, cerebrovascular flow velocity, and heart rate (HR) in sagittal and coronal sections at 1, 2, 3, and 7 days after the operation. For accuracy, cerebral infarct of rats was examined by 2,3,5-Triphenyl tetrazolium chloride (TTC) staining and Nissl staining to simultaneously verify the establishment of NHIE modeling.ResultsCoronal and sagittal TCD scans revealed obvious alteration of cerebrovascular flow in main cerebral vessels. Obvious cerebrovascular back-flow was observed in anterior cerebral artery (ACA), basilar artery (BA), middle cerebral artery (MCA) of HI rats, along with accelerated cerebrovascular flows in the left internal carotid artery (ICA-L) and BA, decreased flows in right internal carotid artery (ICA-R) relative to those in the H and control groups. The alterations of cerebral blood flows in neonatal HI rats indicated successful ligation of right common carotid artery. Besides, TTC staining further validated the cerebral infarct was indeed caused due to ligation-induced insufficient blood supply. Damage to nervous tissues was also revealed by Nissl staining.ConclusionCerebral blood flow assessment by TCD in neonatal HI rats contributed to cerebrovascular abnormalities observed in a real-time and non-invasive way. The present study elicits the potentials to utilize TCD as an effective means for monitoring the progression of injury as well as NHIE modeling. The abnormal appearance of cerebral blood flow is also beneficial to the early warning and effective detection in clinical practice. |
| first_indexed | 2024-04-09T13:14:00Z |
| format | Article |
| id | doaj.art-e3b0be78741146f982e2ff9f9444876d |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-09T13:14:00Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-e3b0be78741146f982e2ff9f9444876d2023-05-12T06:03:21ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2023-05-011710.3389/fnins.2023.962001962001Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modelingJin-Xiang Liu0Jin-Xiang Liu1Chang-Le Fang2Chang-Le Fang3Kun Zhang4Rui-Fang Ma5Hong-Su Zhou6Li Chen7Qiu-Lin Wang8Yu-Xuan Lu9Ting-Hua Wang10Ting-Hua Wang11Liu-Lin Xiong12Liu-Lin Xiong13Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaLaboratory Animal Department, Kunming Medical University, Kunming, ChinaLaboratory Animal Department, Kunming Medical University, Kunming, ChinaSchool of Anesthesiology, Southwest Medical University, Luzhou, ChinaShantou Ultrasonic Instrument Research Institute Co., Ltd., Shantou, Guangdong, ChinaLaboratory Animal Department, Kunming Medical University, Kunming, ChinaDepartment of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Clinical Medicine, Chongqing Medical University, Chongqing, ChinaSchool of Anesthesiology, Southwest Medical University, Luzhou, ChinaLaboratory Animal Department, Kunming Medical University, Kunming, ChinaInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaYunnan Key Laboratory of Primate Biomedical Research, Kunming University of Science and Technology, Kunming, ChinaObjectiveThis study aimed to investigate the feasibility of Transcranial Doppler Ultrasonography (TCD) in evaluating neonatal hypoxic-ischemic encephalopathy (NHIE) modeling through monitoring the alteration of cerebrovascular flow in neonatal hypoxic-ischemic (HI) rats.MethodsPostnatal 7-day-old Sprague Dawley (SD) rats were divided into the control group, HI group, and hypoxia (H) group. TCD was applied to assess the changes of cerebral blood vessels, cerebrovascular flow velocity, and heart rate (HR) in sagittal and coronal sections at 1, 2, 3, and 7 days after the operation. For accuracy, cerebral infarct of rats was examined by 2,3,5-Triphenyl tetrazolium chloride (TTC) staining and Nissl staining to simultaneously verify the establishment of NHIE modeling.ResultsCoronal and sagittal TCD scans revealed obvious alteration of cerebrovascular flow in main cerebral vessels. Obvious cerebrovascular back-flow was observed in anterior cerebral artery (ACA), basilar artery (BA), middle cerebral artery (MCA) of HI rats, along with accelerated cerebrovascular flows in the left internal carotid artery (ICA-L) and BA, decreased flows in right internal carotid artery (ICA-R) relative to those in the H and control groups. The alterations of cerebral blood flows in neonatal HI rats indicated successful ligation of right common carotid artery. Besides, TTC staining further validated the cerebral infarct was indeed caused due to ligation-induced insufficient blood supply. Damage to nervous tissues was also revealed by Nissl staining.ConclusionCerebral blood flow assessment by TCD in neonatal HI rats contributed to cerebrovascular abnormalities observed in a real-time and non-invasive way. The present study elicits the potentials to utilize TCD as an effective means for monitoring the progression of injury as well as NHIE modeling. The abnormal appearance of cerebral blood flow is also beneficial to the early warning and effective detection in clinical practice.https://www.frontiersin.org/articles/10.3389/fnins.2023.962001/fullTranscranial Doppler Ultrasonographyneonatal hypoxic-ischemic encephalopathy modelingcerebrovascular flowcerebral blood vesselscerebral infarct |
| spellingShingle | Jin-Xiang Liu Jin-Xiang Liu Chang-Le Fang Chang-Le Fang Kun Zhang Rui-Fang Ma Hong-Su Zhou Li Chen Qiu-Lin Wang Yu-Xuan Lu Ting-Hua Wang Ting-Hua Wang Liu-Lin Xiong Liu-Lin Xiong Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modeling Frontiers in Neuroscience Transcranial Doppler Ultrasonography neonatal hypoxic-ischemic encephalopathy modeling cerebrovascular flow cerebral blood vessels cerebral infarct |
| title | Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modeling |
| title_full | Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modeling |
| title_fullStr | Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modeling |
| title_full_unstemmed | Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modeling |
| title_short | Transcranial Doppler Ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic-ischemic encephalopathy modeling |
| title_sort | transcranial doppler ultrasonography detection on cerebrovascular flow for evaluating neonatal hypoxic ischemic encephalopathy modeling |
| topic | Transcranial Doppler Ultrasonography neonatal hypoxic-ischemic encephalopathy modeling cerebrovascular flow cerebral blood vessels cerebral infarct |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2023.962001/full |
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