Impact of Algicidal <i>Bacillus mycoides</i> on Diatom <i>Ulnaria acus</i> from Lake Baikal
Algae–bacteria interactions play an important role in water ecosystems. In this work, the BS2-15 algicidal strain was isolated from the bottom sediments of Lake Baikal and identified as <i>Bacillus mycoides</i> on the basis of 16S rDNA sequencing, its described ultrastructure, and bioche...
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MDPI AG
2021-09-01
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| Online Access: | https://www.mdpi.com/1424-2818/13/10/469 |
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| author | Yekaterina Bedoshvili Elvira Bayramova Nikolay Sudakov Igor Klimenkov Maria Kurilkina Yelena Likhoshway Yulia Zakharova |
| author_facet | Yekaterina Bedoshvili Elvira Bayramova Nikolay Sudakov Igor Klimenkov Maria Kurilkina Yelena Likhoshway Yulia Zakharova |
| author_sort | Yekaterina Bedoshvili |
| collection | DOAJ |
| description | Algae–bacteria interactions play an important role in water ecosystems. In this work, the BS2-15 algicidal strain was isolated from the bottom sediments of Lake Baikal and identified as <i>Bacillus mycoides</i> on the basis of 16S rDNA sequencing, its described ultrastructure, and biochemical properties. <i>B. mycoides</i> BS2-15 was demonstrated to have a strong algicidal effect against a freshwater diatom culture of <i>Ulnaria acus,</i> inhibiting its growth and increasing frustules fragility. By analyzing the impact of bacterial filtrate onto the cells of <i>U. acus</i>, we demonstrated that perhaps an algicidal compound is produced by bacteria independently in the presence of diatoms in a medium. Using methods of TUNEL and confocal microscopy, we revealed that the bacterial algicidal effect on the diatom cells results in DNA fragmentation, nucleus destruction, and neutral lipid accumulation. This phenomenon highlights the complexity of algae–bacteria interactions and their potential role in regulating water ecosystem microbial populations. |
| first_indexed | 2024-03-10T06:37:04Z |
| format | Article |
| id | doaj.art-e3b0d39a24fc4e278721e1db1ac9b998 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T06:37:04Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-e3b0d39a24fc4e278721e1db1ac9b9982023-11-22T17:59:20ZengMDPI AGDiversity1424-28182021-09-01131046910.3390/d13100469Impact of Algicidal <i>Bacillus mycoides</i> on Diatom <i>Ulnaria acus</i> from Lake BaikalYekaterina Bedoshvili0Elvira Bayramova1Nikolay Sudakov2Igor Klimenkov3Maria Kurilkina4Yelena Likhoshway5Yulia Zakharova6Department of Cell Ultrastructure, Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaDepartment of Cell Ultrastructure, Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaDepartment of Cell Ultrastructure, Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaDepartment of Cell Ultrastructure, Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaDepartment of Cell Ultrastructure, Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaDepartment of Cell Ultrastructure, Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaDepartment of Cell Ultrastructure, Limnological Institute, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaAlgae–bacteria interactions play an important role in water ecosystems. In this work, the BS2-15 algicidal strain was isolated from the bottom sediments of Lake Baikal and identified as <i>Bacillus mycoides</i> on the basis of 16S rDNA sequencing, its described ultrastructure, and biochemical properties. <i>B. mycoides</i> BS2-15 was demonstrated to have a strong algicidal effect against a freshwater diatom culture of <i>Ulnaria acus,</i> inhibiting its growth and increasing frustules fragility. By analyzing the impact of bacterial filtrate onto the cells of <i>U. acus</i>, we demonstrated that perhaps an algicidal compound is produced by bacteria independently in the presence of diatoms in a medium. Using methods of TUNEL and confocal microscopy, we revealed that the bacterial algicidal effect on the diatom cells results in DNA fragmentation, nucleus destruction, and neutral lipid accumulation. This phenomenon highlights the complexity of algae–bacteria interactions and their potential role in regulating water ecosystem microbial populations.https://www.mdpi.com/1424-2818/13/10/469diatomsalgicidal bacteria<i>Bacillus mycoides</i>TUNEL |
| spellingShingle | Yekaterina Bedoshvili Elvira Bayramova Nikolay Sudakov Igor Klimenkov Maria Kurilkina Yelena Likhoshway Yulia Zakharova Impact of Algicidal <i>Bacillus mycoides</i> on Diatom <i>Ulnaria acus</i> from Lake Baikal Diversity diatoms algicidal bacteria <i>Bacillus mycoides</i> TUNEL |
| title | Impact of Algicidal <i>Bacillus mycoides</i> on Diatom <i>Ulnaria acus</i> from Lake Baikal |
| title_full | Impact of Algicidal <i>Bacillus mycoides</i> on Diatom <i>Ulnaria acus</i> from Lake Baikal |
| title_fullStr | Impact of Algicidal <i>Bacillus mycoides</i> on Diatom <i>Ulnaria acus</i> from Lake Baikal |
| title_full_unstemmed | Impact of Algicidal <i>Bacillus mycoides</i> on Diatom <i>Ulnaria acus</i> from Lake Baikal |
| title_short | Impact of Algicidal <i>Bacillus mycoides</i> on Diatom <i>Ulnaria acus</i> from Lake Baikal |
| title_sort | impact of algicidal i bacillus mycoides i on diatom i ulnaria acus i from lake baikal |
| topic | diatoms algicidal bacteria <i>Bacillus mycoides</i> TUNEL |
| url | https://www.mdpi.com/1424-2818/13/10/469 |
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