Alteration of a Cry1A Shared Binding Site in a Cry1Ab-Selected Colony of <i>Ostrinia furnacalis</i>
The Asian corn borer, <i>Ostrinia furnacalis</i> (Guenée, 1854), is a highly damaging pest in Asia and the Pacific islands, and larvae feed mainly from corn crops. To determine the suitability of Bt-corn technology for the future control of this pest, understanding the potential to devel...
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MDPI AG
2022-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/1/32 |
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| author | Daniel Pinos Yueqin Wang Patricia Hernández-Martínez Kanglai He Juan Ferré |
| author_facet | Daniel Pinos Yueqin Wang Patricia Hernández-Martínez Kanglai He Juan Ferré |
| author_sort | Daniel Pinos |
| collection | DOAJ |
| description | The Asian corn borer, <i>Ostrinia furnacalis</i> (Guenée, 1854), is a highly damaging pest in Asia and the Pacific islands, and larvae feed mainly from corn crops. To determine the suitability of Bt-corn technology for the future control of this pest, understanding the potential to develop resistance to Cry1Ab and the basis of cross-resistance to other Cry1 proteins is of great interest. Here, we have explored the binding of Cry1A proteins to brush border membrane vesicles from two <i>O. furnacalis</i> colonies, one susceptible (ACB-BtS) and one laboratory-selected with Cry1Ab (ACB-AbR). The insects developed resistance to Cry1Ab and showed cross-resistance to Cry1Aa, Cry1Ac, and Cry1F. Binding assays with radiolabeled Cry1Ab and brush border membrane vesicles from susceptible insects showed that Cry1A proteins shared binding sites, though the results were not conclusive for Cry1F. The results were confirmed using radiolabeled Cry1Aa. The resistant insects showed a reduction of the specific binding of both Cry1Ab and Cry1Aa, suggesting that part of the binding sites were lost or altered. Competition binding assays showed full competition between Cry1Ab and Cry1Aa proteins in the susceptible colony but only partial competition in resistant insects, confirming the alteration of some, but not all, binding sites for these two proteins. The binding site model for Cry1A proteins in <i>O. furnacalis</i> is in agreement with the occurrence of multiple membrane receptors for these proteins. |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T00:25:11Z |
| publishDate | 2022-01-01 |
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| series | Toxins |
| spelling | doaj.art-e3b14303d4644c7f817609389f7d98fb2023-11-23T15:35:56ZengMDPI AGToxins2072-66512022-01-011413210.3390/toxins14010032Alteration of a Cry1A Shared Binding Site in a Cry1Ab-Selected Colony of <i>Ostrinia furnacalis</i>Daniel Pinos0Yueqin Wang1Patricia Hernández-Martínez2Kanglai He3Juan Ferré4Instituto de Biotecnología y Biomedicina (BIOTECMED), Deparment of Genetics, Universitat de València, 46100 Burjassot, SpainThe State Key Laboratory for Biology of Plant Diseas and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstituto de Biotecnología y Biomedicina (BIOTECMED), Deparment of Genetics, Universitat de València, 46100 Burjassot, SpainThe State Key Laboratory for Biology of Plant Diseas and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstituto de Biotecnología y Biomedicina (BIOTECMED), Deparment of Genetics, Universitat de València, 46100 Burjassot, SpainThe Asian corn borer, <i>Ostrinia furnacalis</i> (Guenée, 1854), is a highly damaging pest in Asia and the Pacific islands, and larvae feed mainly from corn crops. To determine the suitability of Bt-corn technology for the future control of this pest, understanding the potential to develop resistance to Cry1Ab and the basis of cross-resistance to other Cry1 proteins is of great interest. Here, we have explored the binding of Cry1A proteins to brush border membrane vesicles from two <i>O. furnacalis</i> colonies, one susceptible (ACB-BtS) and one laboratory-selected with Cry1Ab (ACB-AbR). The insects developed resistance to Cry1Ab and showed cross-resistance to Cry1Aa, Cry1Ac, and Cry1F. Binding assays with radiolabeled Cry1Ab and brush border membrane vesicles from susceptible insects showed that Cry1A proteins shared binding sites, though the results were not conclusive for Cry1F. The results were confirmed using radiolabeled Cry1Aa. The resistant insects showed a reduction of the specific binding of both Cry1Ab and Cry1Aa, suggesting that part of the binding sites were lost or altered. Competition binding assays showed full competition between Cry1Ab and Cry1Aa proteins in the susceptible colony but only partial competition in resistant insects, confirming the alteration of some, but not all, binding sites for these two proteins. The binding site model for Cry1A proteins in <i>O. furnacalis</i> is in agreement with the occurrence of multiple membrane receptors for these proteins.https://www.mdpi.com/2072-6651/14/1/32Asian corn borer<i>Bacillus thuringiensis</i>Cry1 toxinsbinding site modelpyramid strategy |
| spellingShingle | Daniel Pinos Yueqin Wang Patricia Hernández-Martínez Kanglai He Juan Ferré Alteration of a Cry1A Shared Binding Site in a Cry1Ab-Selected Colony of <i>Ostrinia furnacalis</i> Toxins Asian corn borer <i>Bacillus thuringiensis</i> Cry1 toxins binding site model pyramid strategy |
| title | Alteration of a Cry1A Shared Binding Site in a Cry1Ab-Selected Colony of <i>Ostrinia furnacalis</i> |
| title_full | Alteration of a Cry1A Shared Binding Site in a Cry1Ab-Selected Colony of <i>Ostrinia furnacalis</i> |
| title_fullStr | Alteration of a Cry1A Shared Binding Site in a Cry1Ab-Selected Colony of <i>Ostrinia furnacalis</i> |
| title_full_unstemmed | Alteration of a Cry1A Shared Binding Site in a Cry1Ab-Selected Colony of <i>Ostrinia furnacalis</i> |
| title_short | Alteration of a Cry1A Shared Binding Site in a Cry1Ab-Selected Colony of <i>Ostrinia furnacalis</i> |
| title_sort | alteration of a cry1a shared binding site in a cry1ab selected colony of i ostrinia furnacalis i |
| topic | Asian corn borer <i>Bacillus thuringiensis</i> Cry1 toxins binding site model pyramid strategy |
| url | https://www.mdpi.com/2072-6651/14/1/32 |
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