Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete <i>Aspergillus ochraceus</i> Capable of Activating Protein C of Blood Plasma
Micromycetes are known to secrete numerous enzymes of biotechnological and medical potential. Fibrinolytic protease-activator of protein C (PAPC) of blood plasma from micromycete <i>Aspergillus ochraceus</i> VKM-F4104D was obtained in recombinant form utilising the bacterial expression s...
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MDPI AG
2021-09-01
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| author | Sergei K. Komarevtsev Peter V. Evseev Mikhail M. Shneider Elizaveta A. Popova Alexey E. Tupikin Vasiliy N. Stepanenko Marsel R. Kabilov Sergei V. Shabunin Alexander A. Osmolovskiy Konstantin A. Miroshnikov |
| author_facet | Sergei K. Komarevtsev Peter V. Evseev Mikhail M. Shneider Elizaveta A. Popova Alexey E. Tupikin Vasiliy N. Stepanenko Marsel R. Kabilov Sergei V. Shabunin Alexander A. Osmolovskiy Konstantin A. Miroshnikov |
| author_sort | Sergei K. Komarevtsev |
| collection | DOAJ |
| description | Micromycetes are known to secrete numerous enzymes of biotechnological and medical potential. Fibrinolytic protease-activator of protein C (PAPC) of blood plasma from micromycete <i>Aspergillus ochraceus</i> VKM-F4104D was obtained in recombinant form utilising the bacterial expression system. This enzyme, which belongs to the proteinase-K-like proteases, is similar to the proteases encoded in the genomes of <i>Aspergillus fumigatus</i> ATCC MYA-4609, <i>A. oryzae</i> ATCC 42149 and <i>A. flavus</i> 28. Mature PAPC-4104 is 282 amino acids long, preceded by the 101-amino acid propeptide necessary for proper folding and maturation. The recombinant protease was identical to the native enzyme from micromycete in terms of its biological properties, including an ability to hydrolyse substrates of activated protein C (pGlu-Pro-Arg-pNA) and factor Xa (Z-D-Arg-Gly-Arg-pNA) in conjugant reactions with human blood plasma. Therefore, recombinant PAPC-4104 can potentially be used in medicine, veterinary science, diagnostics, and other applications. |
| first_indexed | 2024-03-10T07:25:33Z |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:25:33Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e3b64bbdf30f478ba3baa4f374b1df852023-11-22T14:19:22ZengMDPI AGMicroorganisms2076-26072021-09-0199193610.3390/microorganisms9091936Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete <i>Aspergillus ochraceus</i> Capable of Activating Protein C of Blood PlasmaSergei K. Komarevtsev0Peter V. Evseev1Mikhail M. Shneider2Elizaveta A. Popova3Alexey E. Tupikin4Vasiliy N. Stepanenko5Marsel R. Kabilov6Sergei V. Shabunin7Alexander A. Osmolovskiy8Konstantin A. Miroshnikov9Biology Department, Lomonosov Moscow State University, 119234 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaBiology Department, Lomonosov Moscow State University, 119234 Moscow, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaAll-Russian Scientific Research Veterinary Institute of Pathology, Pharmacology and Therapy, 394087 Voronezh, RussiaBiology Department, Lomonosov Moscow State University, 119234 Moscow, RussiaAll-Russian Scientific Research Veterinary Institute of Pathology, Pharmacology and Therapy, 394087 Voronezh, RussiaMicromycetes are known to secrete numerous enzymes of biotechnological and medical potential. Fibrinolytic protease-activator of protein C (PAPC) of blood plasma from micromycete <i>Aspergillus ochraceus</i> VKM-F4104D was obtained in recombinant form utilising the bacterial expression system. This enzyme, which belongs to the proteinase-K-like proteases, is similar to the proteases encoded in the genomes of <i>Aspergillus fumigatus</i> ATCC MYA-4609, <i>A. oryzae</i> ATCC 42149 and <i>A. flavus</i> 28. Mature PAPC-4104 is 282 amino acids long, preceded by the 101-amino acid propeptide necessary for proper folding and maturation. The recombinant protease was identical to the native enzyme from micromycete in terms of its biological properties, including an ability to hydrolyse substrates of activated protein C (pGlu-Pro-Arg-pNA) and factor Xa (Z-D-Arg-Gly-Arg-pNA) in conjugant reactions with human blood plasma. Therefore, recombinant PAPC-4104 can potentially be used in medicine, veterinary science, diagnostics, and other applications.https://www.mdpi.com/2076-2607/9/9/1936fibrinolytic proteaseactivator of protein Cmicromycetegene analysisrecombinant synthesis |
| spellingShingle | Sergei K. Komarevtsev Peter V. Evseev Mikhail M. Shneider Elizaveta A. Popova Alexey E. Tupikin Vasiliy N. Stepanenko Marsel R. Kabilov Sergei V. Shabunin Alexander A. Osmolovskiy Konstantin A. Miroshnikov Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete <i>Aspergillus ochraceus</i> Capable of Activating Protein C of Blood Plasma Microorganisms fibrinolytic protease activator of protein C micromycete gene analysis recombinant synthesis |
| title | Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete <i>Aspergillus ochraceus</i> Capable of Activating Protein C of Blood Plasma |
| title_full | Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete <i>Aspergillus ochraceus</i> Capable of Activating Protein C of Blood Plasma |
| title_fullStr | Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete <i>Aspergillus ochraceus</i> Capable of Activating Protein C of Blood Plasma |
| title_full_unstemmed | Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete <i>Aspergillus ochraceus</i> Capable of Activating Protein C of Blood Plasma |
| title_short | Gene Analysis, Cloning, and Heterologous Expression of Protease from a Micromycete <i>Aspergillus ochraceus</i> Capable of Activating Protein C of Blood Plasma |
| title_sort | gene analysis cloning and heterologous expression of protease from a micromycete i aspergillus ochraceus i capable of activating protein c of blood plasma |
| topic | fibrinolytic protease activator of protein C micromycete gene analysis recombinant synthesis |
| url | https://www.mdpi.com/2076-2607/9/9/1936 |
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