Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease
Objective To analyze the changes in intestinal fungi and compositions in patients with cholestatic liver disease from healthy individuals. Methods Fresh feces from 20 patients with cholestatic liver disease and 22 healthy individuals were collected. The intestinal fungi were determined by ITS2 DNA s...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Army Medical University
2022-06-01
|
| Series: | 陆军军医大学学报 |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202202023.htm |
| _version_ | 1811251436022923264 |
|---|---|
| author | LI Zhen LI Zhen YANG Guodong TANG Bo TANG Li |
| author_facet | LI Zhen LI Zhen YANG Guodong TANG Bo TANG Li |
| author_sort | LI Zhen |
| collection | DOAJ |
| description | Objective To analyze the changes in intestinal fungi and compositions in patients with cholestatic liver disease from healthy individuals. Methods Fresh feces from 20 patients with cholestatic liver disease and 22 healthy individuals were collected. The intestinal fungi were determined by ITS2 DNA sequencing, and the differences of fungal flora diversity, structure and functional characteristics between the 2 groups were analyzed by bioinformatics methods, and the correlation between intestinal fungi and clinical parameters was analyzed. Results There were no significant differences in age, sex composition and smoking between the 2 groups (P>0.05). The serological indexes were significantly higher in the patients with cholestatic liver disease than the healthy controls (P < 0.05). No significant difference was seen in the Alpha diversity of intestinal fungi between the 2 groups (P>0.05), but significant differences were observed in Beta diversity (P < 0.01). The relative abundance of Myceliophthora was increased (P < 0.01) and that of Diutina was decreased (P < 0.01) in the patients with cholestatic liver disease. LEfSe analysis showed that Myceliophthora was significantly enriched in the patients, and the abundance of Saprotroph-Symbiotroph was significantly lower in the patients than the healthy controls. Spearman correlation coefficient analysis showed that Myceliophthora was positively correlated with alanine transaminase (ALT, P < 0.05), and aspartate transaminase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT) and total bilirubin (TBIL) (P < 0.01). Conclusion Characteristic changes are seen in intestinal fungal compositions between the patients with cholestatic liver disease and the healthy controls. Myceliophthora is closely associated with the severity of the disease.
|
| first_indexed | 2024-04-12T16:20:26Z |
| format | Article |
| id | doaj.art-e3b75ac9037a4579944cda257e50989f |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-12T16:20:26Z |
| publishDate | 2022-06-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-e3b75ac9037a4579944cda257e50989f2022-12-22T03:25:35ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272022-06-0144111162116410.16016/j.2097-0927.202202023Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease LI Zhen0LI Zhen1YANG Guodong2TANG Bo3TANG Li4Department of Pathogen Biology, College of Basic Medicine and Forensic Medicine, Affiliated Hospital, North Sichuan Medical College, Nanchong, Sichuan Province, 637000Department of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, ChinaDepartment of Gastroenterology, Affiliated Hospital, North Sichuan Medical College, Nanchong, Sichuan Province, 637000 Department of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, ChinaDepartment of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, ChinaObjective To analyze the changes in intestinal fungi and compositions in patients with cholestatic liver disease from healthy individuals. Methods Fresh feces from 20 patients with cholestatic liver disease and 22 healthy individuals were collected. The intestinal fungi were determined by ITS2 DNA sequencing, and the differences of fungal flora diversity, structure and functional characteristics between the 2 groups were analyzed by bioinformatics methods, and the correlation between intestinal fungi and clinical parameters was analyzed. Results There were no significant differences in age, sex composition and smoking between the 2 groups (P>0.05). The serological indexes were significantly higher in the patients with cholestatic liver disease than the healthy controls (P < 0.05). No significant difference was seen in the Alpha diversity of intestinal fungi between the 2 groups (P>0.05), but significant differences were observed in Beta diversity (P < 0.01). The relative abundance of Myceliophthora was increased (P < 0.01) and that of Diutina was decreased (P < 0.01) in the patients with cholestatic liver disease. LEfSe analysis showed that Myceliophthora was significantly enriched in the patients, and the abundance of Saprotroph-Symbiotroph was significantly lower in the patients than the healthy controls. Spearman correlation coefficient analysis showed that Myceliophthora was positively correlated with alanine transaminase (ALT, P < 0.05), and aspartate transaminase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT) and total bilirubin (TBIL) (P < 0.01). Conclusion Characteristic changes are seen in intestinal fungal compositions between the patients with cholestatic liver disease and the healthy controls. Myceliophthora is closely associated with the severity of the disease. http://aammt.tmmu.edu.cn/Upload/rhtml/202202023.htmcholestatic liver diseasefungiits2 dna |
| spellingShingle | LI Zhen LI Zhen YANG Guodong TANG Bo TANG Li Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease 陆军军医大学学报 cholestatic liver disease fungi its2 dna |
| title | Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease |
| title_full | Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease |
| title_fullStr | Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease |
| title_full_unstemmed | Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease |
| title_short | Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease |
| title_sort | changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease |
| topic | cholestatic liver disease fungi its2 dna |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202202023.htm |
| work_keys_str_mv | AT lizhen changesinintestinalfungaldiversityandcompositionsinpatientswithcholestaticliverdisease AT lizhen changesinintestinalfungaldiversityandcompositionsinpatientswithcholestaticliverdisease AT yangguodong changesinintestinalfungaldiversityandcompositionsinpatientswithcholestaticliverdisease AT tangbo changesinintestinalfungaldiversityandcompositionsinpatientswithcholestaticliverdisease AT tangli changesinintestinalfungaldiversityandcompositionsinpatientswithcholestaticliverdisease |