Optical imaging of the peri-tumoral inflammatory response in breast cancer
<p>Abstract</p> <p>Purpose</p> <p>Peri-tumoral inflammation is a common tumor response that plays a central role in tumor invasion and metastasis, and inflammatory cell recruitment is essential to this process. The purpose of this study was to determine whether injected...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-11-01
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| Series: | Journal of Translational Medicine |
| Online Access: | http://www.translational-medicine.com/content/7/1/94 |
| _version_ | 1818118065913593856 |
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| author | Reinhart Verena Ansari Celina Kishore Sirish A Boddington Sophie E DeNardo David G Johansson Magnus Tavri Sidhartha Knebel Robert J Sista Akhilesh K Coakley Fergus V Coussens Lisa M Daldrup-Link Heike E |
| author_facet | Reinhart Verena Ansari Celina Kishore Sirish A Boddington Sophie E DeNardo David G Johansson Magnus Tavri Sidhartha Knebel Robert J Sista Akhilesh K Coakley Fergus V Coussens Lisa M Daldrup-Link Heike E |
| author_sort | Reinhart Verena |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Purpose</p> <p>Peri-tumoral inflammation is a common tumor response that plays a central role in tumor invasion and metastasis, and inflammatory cell recruitment is essential to this process. The purpose of this study was to determine whether injected fluorescently-labeled monocytes accumulate within murine breast tumors and are visible with optical imaging.</p> <p>Materials and methods</p> <p>Murine monocytes were labeled with the fluorescent dye DiD and subsequently injected intravenously into 6 transgenic MMTV-PymT tumor-bearing mice and 6 FVB/n control mice without tumors. Optical imaging (OI) was performed before and after cell injection. Ratios of post-injection to pre-injection fluorescent signal intensity of the tumors (MMTV-PymT mice) and mammary tissue (FVB/n controls) were calculated and statistically compared.</p> <p>Results</p> <p>MMTV-PymT breast tumors had an average post/pre signal intensity ratio of 1.8+/- 0.2 (range 1.1-2.7). Control mammary tissue had an average post/pre signal intensity ratio of 1.1 +/- 0.1 (range, 0.4 to 1.4). The p-value for the difference between the ratios was less than 0.05. Confocal fluorescence microscopy confirmed the presence of DiD-labeled cells within the breast tumors.</p> <p>Conclusion</p> <p>Murine monocytes accumulate at the site of breast cancer development in this transgenic model, providing evidence that peri-tumoral inflammatory cell recruitment can be evaluated non-invasively using optical imaging.</p> |
| first_indexed | 2024-12-11T04:48:23Z |
| format | Article |
| id | doaj.art-e3b879a677f44b7fa0bf1ad83151e09c |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-11T04:48:23Z |
| publishDate | 2009-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-e3b879a677f44b7fa0bf1ad83151e09c2022-12-22T01:20:27ZengBMCJournal of Translational Medicine1479-58762009-11-01719410.1186/1479-5876-7-94Optical imaging of the peri-tumoral inflammatory response in breast cancerReinhart VerenaAnsari CelinaKishore Sirish ABoddington Sophie EDeNardo David GJohansson MagnusTavri SidharthaKnebel Robert JSista Akhilesh KCoakley Fergus VCoussens Lisa MDaldrup-Link Heike E<p>Abstract</p> <p>Purpose</p> <p>Peri-tumoral inflammation is a common tumor response that plays a central role in tumor invasion and metastasis, and inflammatory cell recruitment is essential to this process. The purpose of this study was to determine whether injected fluorescently-labeled monocytes accumulate within murine breast tumors and are visible with optical imaging.</p> <p>Materials and methods</p> <p>Murine monocytes were labeled with the fluorescent dye DiD and subsequently injected intravenously into 6 transgenic MMTV-PymT tumor-bearing mice and 6 FVB/n control mice without tumors. Optical imaging (OI) was performed before and after cell injection. Ratios of post-injection to pre-injection fluorescent signal intensity of the tumors (MMTV-PymT mice) and mammary tissue (FVB/n controls) were calculated and statistically compared.</p> <p>Results</p> <p>MMTV-PymT breast tumors had an average post/pre signal intensity ratio of 1.8+/- 0.2 (range 1.1-2.7). Control mammary tissue had an average post/pre signal intensity ratio of 1.1 +/- 0.1 (range, 0.4 to 1.4). The p-value for the difference between the ratios was less than 0.05. Confocal fluorescence microscopy confirmed the presence of DiD-labeled cells within the breast tumors.</p> <p>Conclusion</p> <p>Murine monocytes accumulate at the site of breast cancer development in this transgenic model, providing evidence that peri-tumoral inflammatory cell recruitment can be evaluated non-invasively using optical imaging.</p>http://www.translational-medicine.com/content/7/1/94 |
| spellingShingle | Reinhart Verena Ansari Celina Kishore Sirish A Boddington Sophie E DeNardo David G Johansson Magnus Tavri Sidhartha Knebel Robert J Sista Akhilesh K Coakley Fergus V Coussens Lisa M Daldrup-Link Heike E Optical imaging of the peri-tumoral inflammatory response in breast cancer Journal of Translational Medicine |
| title | Optical imaging of the peri-tumoral inflammatory response in breast cancer |
| title_full | Optical imaging of the peri-tumoral inflammatory response in breast cancer |
| title_fullStr | Optical imaging of the peri-tumoral inflammatory response in breast cancer |
| title_full_unstemmed | Optical imaging of the peri-tumoral inflammatory response in breast cancer |
| title_short | Optical imaging of the peri-tumoral inflammatory response in breast cancer |
| title_sort | optical imaging of the peri tumoral inflammatory response in breast cancer |
| url | http://www.translational-medicine.com/content/7/1/94 |
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