Quercetin-Rich Extracts from Onions (<i>Allium cepa</i>) Play Potent Cytotoxicity on Adrenocortical Carcinoma Cell Lines, and Quercetin Induces Important Anticancer Properties
Adrenocortical carcinoma (ACC) is a rare subtype of cancer, with a poor prognosis in children and adults. Mitotane is the only approved adrenolytic drug for the treatment of ACC, which has controversies regarding its efficacy and side effects on patients. Onion (<i>Allium cepa</i>), a wo...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/6/754 |
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| author | Alan A. Veiga Ana Carolina Irioda Bassam F. Mogharbel Sandro J. R. Bonatto Lauro M. Souza |
| author_facet | Alan A. Veiga Ana Carolina Irioda Bassam F. Mogharbel Sandro J. R. Bonatto Lauro M. Souza |
| author_sort | Alan A. Veiga |
| collection | DOAJ |
| description | Adrenocortical carcinoma (ACC) is a rare subtype of cancer, with a poor prognosis in children and adults. Mitotane is the only approved adrenolytic drug for the treatment of ACC, which has controversies regarding its efficacy and side effects on patients. Onion (<i>Allium cepa</i>), a worldwide consumed food, is associated with many health benefits. Along with its glycosides, the flavonoid quercetin is abundant in onions. After evaluating the cytotoxicity of <i>A. cepa</i> extracts on adrenocortical carcinoma cell line (H295R), the rich quercetin fractions had better results. Then, we aimed to compare the quercetin vs. mitotane effectiveness, using adrenocortical carcinoma cell lines H295R and SW-13. Quercetin showed a higher cytotoxicity response on both cancerous cell lines after 10 µM concentration, while mitotane only after 30 µM. Cell cycle dynamics were altered upon quercetin treatments, with G2 phase increase with 30 µM of quercetin on H295R cell line and G1 arrest on SW-13 cell line with 15 µM. Early and late apoptosis, alongside intracellular calcium, were increased on SW-13 treated with 30 µM of quercetin, and ROS rates were reduced by quercetin on H295R. Therefore, quercetin-rich onions have the potential to be a natural source of anticancer agents for adrenocortical carcinoma. |
| first_indexed | 2024-03-09T22:46:56Z |
| format | Article |
| id | doaj.art-e3b886ba96884b1a9df0c3a0584db2a9 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T22:46:56Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-e3b886ba96884b1a9df0c3a0584db2a92023-11-23T18:28:09ZengMDPI AGPharmaceuticals1424-82472022-06-0115675410.3390/ph15060754Quercetin-Rich Extracts from Onions (<i>Allium cepa</i>) Play Potent Cytotoxicity on Adrenocortical Carcinoma Cell Lines, and Quercetin Induces Important Anticancer PropertiesAlan A. Veiga0Ana Carolina Irioda1Bassam F. Mogharbel2Sandro J. R. Bonatto3Lauro M. Souza4Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80250-060, BrazilInstituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80250-060, BrazilInstituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80250-060, BrazilInstituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80250-060, BrazilInstituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80250-060, BrazilAdrenocortical carcinoma (ACC) is a rare subtype of cancer, with a poor prognosis in children and adults. Mitotane is the only approved adrenolytic drug for the treatment of ACC, which has controversies regarding its efficacy and side effects on patients. Onion (<i>Allium cepa</i>), a worldwide consumed food, is associated with many health benefits. Along with its glycosides, the flavonoid quercetin is abundant in onions. After evaluating the cytotoxicity of <i>A. cepa</i> extracts on adrenocortical carcinoma cell line (H295R), the rich quercetin fractions had better results. Then, we aimed to compare the quercetin vs. mitotane effectiveness, using adrenocortical carcinoma cell lines H295R and SW-13. Quercetin showed a higher cytotoxicity response on both cancerous cell lines after 10 µM concentration, while mitotane only after 30 µM. Cell cycle dynamics were altered upon quercetin treatments, with G2 phase increase with 30 µM of quercetin on H295R cell line and G1 arrest on SW-13 cell line with 15 µM. Early and late apoptosis, alongside intracellular calcium, were increased on SW-13 treated with 30 µM of quercetin, and ROS rates were reduced by quercetin on H295R. Therefore, quercetin-rich onions have the potential to be a natural source of anticancer agents for adrenocortical carcinoma.https://www.mdpi.com/1424-8247/15/6/754cancerantitumor agentsflavonoidsH295RSW-13onions |
| spellingShingle | Alan A. Veiga Ana Carolina Irioda Bassam F. Mogharbel Sandro J. R. Bonatto Lauro M. Souza Quercetin-Rich Extracts from Onions (<i>Allium cepa</i>) Play Potent Cytotoxicity on Adrenocortical Carcinoma Cell Lines, and Quercetin Induces Important Anticancer Properties Pharmaceuticals cancer antitumor agents flavonoids H295R SW-13 onions |
| title | Quercetin-Rich Extracts from Onions (<i>Allium cepa</i>) Play Potent Cytotoxicity on Adrenocortical Carcinoma Cell Lines, and Quercetin Induces Important Anticancer Properties |
| title_full | Quercetin-Rich Extracts from Onions (<i>Allium cepa</i>) Play Potent Cytotoxicity on Adrenocortical Carcinoma Cell Lines, and Quercetin Induces Important Anticancer Properties |
| title_fullStr | Quercetin-Rich Extracts from Onions (<i>Allium cepa</i>) Play Potent Cytotoxicity on Adrenocortical Carcinoma Cell Lines, and Quercetin Induces Important Anticancer Properties |
| title_full_unstemmed | Quercetin-Rich Extracts from Onions (<i>Allium cepa</i>) Play Potent Cytotoxicity on Adrenocortical Carcinoma Cell Lines, and Quercetin Induces Important Anticancer Properties |
| title_short | Quercetin-Rich Extracts from Onions (<i>Allium cepa</i>) Play Potent Cytotoxicity on Adrenocortical Carcinoma Cell Lines, and Quercetin Induces Important Anticancer Properties |
| title_sort | quercetin rich extracts from onions i allium cepa i play potent cytotoxicity on adrenocortical carcinoma cell lines and quercetin induces important anticancer properties |
| topic | cancer antitumor agents flavonoids H295R SW-13 onions |
| url | https://www.mdpi.com/1424-8247/15/6/754 |
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