Diagnosis of Helicobacter pylori Infection in Children and Adult by Serological Test and Stool Antigen Test
Background: Helicobacter pylori infection causes chronic gastritis that is related to duodenal ulcer, gastric ulcer, and possibly gastric adenocarcinoma. Noninvasive diagnostic tests consist of the urea breath test, serology, and stool antigen testing. Serodiagnosis of H.pylori infection is inaccura...
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| Format: | Article |
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Alborz University of Medical Sciencs
2013-10-01
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| Series: | Bihdād |
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| Online Access: | http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-51&slc_lang=en&sid=1 |
| _version_ | 1811324648498921472 |
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| author | G. Azizi M. Nasiri R. Sadria MH. Javanbakht M. Kalvandi N Khannazer B. Asghari |
| author_facet | G. Azizi M. Nasiri R. Sadria MH. Javanbakht M. Kalvandi N Khannazer B. Asghari |
| author_sort | G. Azizi |
| collection | DOAJ |
| description | Background: Helicobacter pylori infection causes chronic gastritis that is related to duodenal ulcer, gastric ulcer, and possibly gastric adenocarcinoma. Noninvasive diagnostic tests consist of the urea breath test, serology, and stool antigen testing. Serodiagnosis of H.pylori infection is inaccurate for children. In order to investigate the immune response to H.pylori in children and adult, we compared anti-H pylori IgG and IgA antibodies with H. pylori antigen (HpSA) in the stool. Methods: Serum and stool samples were obtained from 218 children and adult patients with clinical symptom in the range of 4 to 77 years old. Paired results of H. pylori serology (IgG and IgA) and HpSA were analyzed by enzyme immunoassay methods. Results: There were 218 paired serology and HpSA results for 39 children (≤17 years) and 179 adult (≥18 years). The positivity rate of HpSA (45.8%) was significantly lower (P<0.001) than those for H. pylori IgG (54.6%) and IgA (28.9%). Moreover in child patients specificity for serological test IgG and IgA were higher than adult. Conclusion: In this study, HpSA was sensitive and specific as a clinical and epidemiological tool to evaluate H. pylori infection. IgG correlated better with HpSA than IgA, and also IgG was much more specific in children than adults confirm the fact that adults are more possible to have been exposed to H. pylori in the past. Using HpSA as the gold standard, we found that the performances of IgG and IgA serology tests differ significantly by age because immature immune response or tolerance to H. pylori is present in childhood and serodiagnosis of H. pylori infection is less useful. Hence, we recommend that laboratories reevaluate reference serologic titers based on age and further clinical correlation is needed to establish the optimal ranges. |
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| issn | 1735-6679 |
| language | fas |
| last_indexed | 2024-04-13T14:19:10Z |
| publishDate | 2013-10-01 |
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| record_format | Article |
| series | Bihdād |
| spelling | doaj.art-e3b9db1198604565a89087344108e18f2022-12-22T02:43:34ZfasAlborz University of Medical SciencsBihdād1735-66792013-10-0124191196Diagnosis of Helicobacter pylori Infection in Children and Adult by Serological Test and Stool Antigen TestG. Azizi0M. Nasiri1R. Sadria2MH. Javanbakht3M. Kalvandi4N Khannazer5B. Asghari6 Immunology MSc, Imam Hassan Mojtaba Hospital, Alborz University of Medical Sciences, Karaj, Iran Immunology technician, Imam Hassan Mojtaba Hospital, Alborz University of Medical Sciences, Karaj, Iran Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Nutrition and Biochemistry, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Shahriar Tamin Ejtemaee Hospital, Tehran Tamin Ejtemaee Organization, Tehran, Iran Department of Molecular and Cellular Biology, College of Science, University of Tehran, Tehran, Iran Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Background: Helicobacter pylori infection causes chronic gastritis that is related to duodenal ulcer, gastric ulcer, and possibly gastric adenocarcinoma. Noninvasive diagnostic tests consist of the urea breath test, serology, and stool antigen testing. Serodiagnosis of H.pylori infection is inaccurate for children. In order to investigate the immune response to H.pylori in children and adult, we compared anti-H pylori IgG and IgA antibodies with H. pylori antigen (HpSA) in the stool. Methods: Serum and stool samples were obtained from 218 children and adult patients with clinical symptom in the range of 4 to 77 years old. Paired results of H. pylori serology (IgG and IgA) and HpSA were analyzed by enzyme immunoassay methods. Results: There were 218 paired serology and HpSA results for 39 children (≤17 years) and 179 adult (≥18 years). The positivity rate of HpSA (45.8%) was significantly lower (P<0.001) than those for H. pylori IgG (54.6%) and IgA (28.9%). Moreover in child patients specificity for serological test IgG and IgA were higher than adult. Conclusion: In this study, HpSA was sensitive and specific as a clinical and epidemiological tool to evaluate H. pylori infection. IgG correlated better with HpSA than IgA, and also IgG was much more specific in children than adults confirm the fact that adults are more possible to have been exposed to H. pylori in the past. Using HpSA as the gold standard, we found that the performances of IgG and IgA serology tests differ significantly by age because immature immune response or tolerance to H. pylori is present in childhood and serodiagnosis of H. pylori infection is less useful. Hence, we recommend that laboratories reevaluate reference serologic titers based on age and further clinical correlation is needed to establish the optimal ranges.http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-51&slc_lang=en&sid=1Helicobacter pylori Serology tests Sensitivity Specificity Peptic ulcer |
| spellingShingle | G. Azizi M. Nasiri R. Sadria MH. Javanbakht M. Kalvandi N Khannazer B. Asghari Diagnosis of Helicobacter pylori Infection in Children and Adult by Serological Test and Stool Antigen Test Bihdād Helicobacter pylori Serology tests Sensitivity Specificity Peptic ulcer |
| title | Diagnosis of Helicobacter pylori Infection in Children and Adult by Serological Test and Stool Antigen Test |
| title_full | Diagnosis of Helicobacter pylori Infection in Children and Adult by Serological Test and Stool Antigen Test |
| title_fullStr | Diagnosis of Helicobacter pylori Infection in Children and Adult by Serological Test and Stool Antigen Test |
| title_full_unstemmed | Diagnosis of Helicobacter pylori Infection in Children and Adult by Serological Test and Stool Antigen Test |
| title_short | Diagnosis of Helicobacter pylori Infection in Children and Adult by Serological Test and Stool Antigen Test |
| title_sort | diagnosis of helicobacter pylori infection in children and adult by serological test and stool antigen test |
| topic | Helicobacter pylori Serology tests Sensitivity Specificity Peptic ulcer |
| url | http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-51&slc_lang=en&sid=1 |
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