Allosteric communication in DNA polymerase clamp loaders relies on a critical hydrogen-bonded junction

Allosteric communication in DNA polymerase clamp loaders relies on a critical hydrogen-bonded junction

Clamp loaders are AAA+ ATPases that load sliding clamps onto DNA. We mapped the mutational sensitivity of the T4 bacteriophage sliding clamp and clamp loader by deep mutagenesis, and found that residues not involved in catalysis or binding display remarkable tolerance to mutation. An exception is a...

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Bibliographic Details
Main Authors: Subu Subramanian, Kent Gorday, Kendra Marcus, Matthew R Orellana, Peter Ren, Xiao Ran Luo, Michael E O'Donnell, John Kuriyan
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-04-01
Series:eLife
Subjects:
bacteriophage T4
AAA+ proteins
allosteric communication
replisome
Online Access:https://elifesciences.org/articles/66181

Internet

https://elifesciences.org/articles/66181

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