Genome-Wide Identification and Expression Patterns of the <i>SWEET</i> Gene Family in <i>Bletilla striata</i> and its Responses to Low Temperature and Oxidative Stress
SWEETs (sugars will eventually be exported transporters), a well-known class of sugar transporters, are involved in plant growth and development, sugar transport, biotic and abiotic stresses, etc. However, to date, there have been few investigations of <i>SWEETs</i> in Orchidaceae. In th...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/17/10057 |
| _version_ | 1797495035058454528 |
|---|---|
| author | Chan Lu Jun Ye Yuanqing Chang Zeyuan Mi Shuai Liu Donghao Wang Zhezhi Wang Junfeng Niu |
| author_facet | Chan Lu Jun Ye Yuanqing Chang Zeyuan Mi Shuai Liu Donghao Wang Zhezhi Wang Junfeng Niu |
| author_sort | Chan Lu |
| collection | DOAJ |
| description | SWEETs (sugars will eventually be exported transporters), a well-known class of sugar transporters, are involved in plant growth and development, sugar transport, biotic and abiotic stresses, etc. However, to date, there have been few investigations of <i>SWEETs</i> in Orchidaceae. In this study, 23 <i>SWEET</i> genes were identified in <i>Bletilla striata</i> for the first time, with an MtN3/saliva conserved domain, and were divided into four subgroups by phylogenetic tree. The same subfamily members had similar gene structures and motifs. Multiple <i>cis</i>-elements related to sugar and environmental stresses were found in the promoter region. Further, 21 genes were localized on 11 chromosomes and 2 paralogous pairs were found via intraspecific collinearity analysis. Expression profiling results showed that <i>BsSWEETs</i> were tissue-specific. It also revealed that <i>BsSWEET10</i> and <i>BsSWEET18</i> were responsive to low temperature and oxidative stresses. In addition, subcellular localization study indicated that <i>BsSWEET15</i> and <i>BsSWEET16</i> were localized in the cell membrane. This study provided important clues for the in-depth elucidation of the sugar transport mechanism of <i>BsSWEET</i> genes and their functional roles in response to abiotic stresses. |
| first_indexed | 2024-03-10T01:42:45Z |
| format | Article |
| id | doaj.art-e3bcf4d9c694464eb70d9a1ed586fa86 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:42:45Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e3bcf4d9c694464eb70d9a1ed586fa862023-11-23T13:22:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123171005710.3390/ijms231710057Genome-Wide Identification and Expression Patterns of the <i>SWEET</i> Gene Family in <i>Bletilla striata</i> and its Responses to Low Temperature and Oxidative StressChan Lu0Jun Ye1Yuanqing Chang2Zeyuan Mi3Shuai Liu4Donghao Wang5Zhezhi Wang6Junfeng Niu7National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, ChinaSWEETs (sugars will eventually be exported transporters), a well-known class of sugar transporters, are involved in plant growth and development, sugar transport, biotic and abiotic stresses, etc. However, to date, there have been few investigations of <i>SWEETs</i> in Orchidaceae. In this study, 23 <i>SWEET</i> genes were identified in <i>Bletilla striata</i> for the first time, with an MtN3/saliva conserved domain, and were divided into four subgroups by phylogenetic tree. The same subfamily members had similar gene structures and motifs. Multiple <i>cis</i>-elements related to sugar and environmental stresses were found in the promoter region. Further, 21 genes were localized on 11 chromosomes and 2 paralogous pairs were found via intraspecific collinearity analysis. Expression profiling results showed that <i>BsSWEETs</i> were tissue-specific. It also revealed that <i>BsSWEET10</i> and <i>BsSWEET18</i> were responsive to low temperature and oxidative stresses. In addition, subcellular localization study indicated that <i>BsSWEET15</i> and <i>BsSWEET16</i> were localized in the cell membrane. This study provided important clues for the in-depth elucidation of the sugar transport mechanism of <i>BsSWEET</i> genes and their functional roles in response to abiotic stresses.https://www.mdpi.com/1422-0067/23/17/10057<i>Bletilla striata</i>expression patternsubcellular localization<i>SWEET</i> |
| spellingShingle | Chan Lu Jun Ye Yuanqing Chang Zeyuan Mi Shuai Liu Donghao Wang Zhezhi Wang Junfeng Niu Genome-Wide Identification and Expression Patterns of the <i>SWEET</i> Gene Family in <i>Bletilla striata</i> and its Responses to Low Temperature and Oxidative Stress International Journal of Molecular Sciences <i>Bletilla striata</i> expression pattern subcellular localization <i>SWEET</i> |
| title | Genome-Wide Identification and Expression Patterns of the <i>SWEET</i> Gene Family in <i>Bletilla striata</i> and its Responses to Low Temperature and Oxidative Stress |
| title_full | Genome-Wide Identification and Expression Patterns of the <i>SWEET</i> Gene Family in <i>Bletilla striata</i> and its Responses to Low Temperature and Oxidative Stress |
| title_fullStr | Genome-Wide Identification and Expression Patterns of the <i>SWEET</i> Gene Family in <i>Bletilla striata</i> and its Responses to Low Temperature and Oxidative Stress |
| title_full_unstemmed | Genome-Wide Identification and Expression Patterns of the <i>SWEET</i> Gene Family in <i>Bletilla striata</i> and its Responses to Low Temperature and Oxidative Stress |
| title_short | Genome-Wide Identification and Expression Patterns of the <i>SWEET</i> Gene Family in <i>Bletilla striata</i> and its Responses to Low Temperature and Oxidative Stress |
| title_sort | genome wide identification and expression patterns of the i sweet i gene family in i bletilla striata i and its responses to low temperature and oxidative stress |
| topic | <i>Bletilla striata</i> expression pattern subcellular localization <i>SWEET</i> |
| url | https://www.mdpi.com/1422-0067/23/17/10057 |
| work_keys_str_mv | AT chanlu genomewideidentificationandexpressionpatternsoftheisweetigenefamilyinibletillastriataianditsresponsestolowtemperatureandoxidativestress AT junye genomewideidentificationandexpressionpatternsoftheisweetigenefamilyinibletillastriataianditsresponsestolowtemperatureandoxidativestress AT yuanqingchang genomewideidentificationandexpressionpatternsoftheisweetigenefamilyinibletillastriataianditsresponsestolowtemperatureandoxidativestress AT zeyuanmi genomewideidentificationandexpressionpatternsoftheisweetigenefamilyinibletillastriataianditsresponsestolowtemperatureandoxidativestress AT shuailiu genomewideidentificationandexpressionpatternsoftheisweetigenefamilyinibletillastriataianditsresponsestolowtemperatureandoxidativestress AT donghaowang genomewideidentificationandexpressionpatternsoftheisweetigenefamilyinibletillastriataianditsresponsestolowtemperatureandoxidativestress AT zhezhiwang genomewideidentificationandexpressionpatternsoftheisweetigenefamilyinibletillastriataianditsresponsestolowtemperatureandoxidativestress AT junfengniu genomewideidentificationandexpressionpatternsoftheisweetigenefamilyinibletillastriataianditsresponsestolowtemperatureandoxidativestress |