Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System

Aim: The aim of the study was to compare the antimicrobial efficacy of three irrigation techniques after the use of standardized volume of NaOCl and with standardized time and irrigation. Methodology: Forty-eight single rooted teeth were inoculated with an Enterococcus faecalis suspension for 24 h....

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Main Authors: Ivana Toljan, Ivona Bago, Ivica Anić
Format: Article
Language:English
Published: University of Zagreb. School of Dental Medicine 2016-01-01
Series:Acta Stomatologica Croatica
Subjects:
Online Access:https://hrcak.srce.hr/file/227452
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author Ivana Toljan
Ivona Bago
Ivica Anić
author_facet Ivana Toljan
Ivona Bago
Ivica Anić
author_sort Ivana Toljan
collection DOAJ
description Aim: The aim of the study was to compare the antimicrobial efficacy of three irrigation techniques after the use of standardized volume of NaOCl and with standardized time and irrigation. Methodology: Forty-eight single rooted teeth were inoculated with an Enterococcus faecalis suspension for 24 h. The remaining six canals served as negative controls. The 36 root canals were randomly distributed into three experimental groups; group 1, conventional syringe irrigation; group 2, automateddynamic irrigation (RinsEndo); group 3, passive ultrasonic irrigation (PUI). In the first protocol, the standardized volume of 3% NaOCl (20 mL) was used and in the second protocol, and standardized irrigation time (45 seconds) was used. Samples from root canals were cultured and the colony-forming units (CFUs) were counted. Results: When the volume of the irrigant was tandardized, RinsEndo was more effective than PUI (p<0.01). When the irrigation time was standardized, there were no significant differences between any irrigation techniques (p>0.05). The RinsEndo group had the highest percentage of minimal counts of E. faecalis CFUs. Conclusions: RinsEndo was more effective than PUI only when the volume of the irrigant was standardized. However, the RinsEndo provided higher bacterial reduction in both protocols when using the least amount of the irrigant and providing longer contact time.
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spelling doaj.art-e3bd865ad7f44eb284a538688f213c492024-04-15T13:46:46ZengUniversity of Zagreb. School of Dental MedicineActa Stomatologica Croatica0001-70191846-04102016-01-01501142210.15644/asc50/1/3Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo SystemIvana Toljan0Ivona Bago1Ivica Anić2Private Dental Polyclinic, Zagreb, CroatiaDepartment of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, CroatiaDepartment of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, CroatiaAim: The aim of the study was to compare the antimicrobial efficacy of three irrigation techniques after the use of standardized volume of NaOCl and with standardized time and irrigation. Methodology: Forty-eight single rooted teeth were inoculated with an Enterococcus faecalis suspension for 24 h. The remaining six canals served as negative controls. The 36 root canals were randomly distributed into three experimental groups; group 1, conventional syringe irrigation; group 2, automateddynamic irrigation (RinsEndo); group 3, passive ultrasonic irrigation (PUI). In the first protocol, the standardized volume of 3% NaOCl (20 mL) was used and in the second protocol, and standardized irrigation time (45 seconds) was used. Samples from root canals were cultured and the colony-forming units (CFUs) were counted. Results: When the volume of the irrigant was tandardized, RinsEndo was more effective than PUI (p<0.01). When the irrigation time was standardized, there were no significant differences between any irrigation techniques (p>0.05). The RinsEndo group had the highest percentage of minimal counts of E. faecalis CFUs. Conclusions: RinsEndo was more effective than PUI only when the volume of the irrigant was standardized. However, the RinsEndo provided higher bacterial reduction in both protocols when using the least amount of the irrigant and providing longer contact time.https://hrcak.srce.hr/file/227452Root Canal PreparationRoot Canal IrrigantsEnterococcus faecalis
spellingShingle Ivana Toljan
Ivona Bago
Ivica Anić
Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System
Acta Stomatologica Croatica
Root Canal Preparation
Root Canal Irrigants
Enterococcus faecalis
title Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System
title_full Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System
title_fullStr Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System
title_full_unstemmed Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System
title_short Eradication of Intracanal Enterococcus Faecalis Biofilm by Passive Ultrasonic Irrigation and RinsEndo System
title_sort eradication of intracanal enterococcus faecalis biofilm by passive ultrasonic irrigation and rinsendo system
topic Root Canal Preparation
Root Canal Irrigants
Enterococcus faecalis
url https://hrcak.srce.hr/file/227452
work_keys_str_mv AT ivanatoljan eradicationofintracanalenterococcusfaecalisbiofilmbypassiveultrasonicirrigationandrinsendosystem
AT ivonabago eradicationofintracanalenterococcusfaecalisbiofilmbypassiveultrasonicirrigationandrinsendosystem
AT ivicaanic eradicationofintracanalenterococcusfaecalisbiofilmbypassiveultrasonicirrigationandrinsendosystem