Inhibition effects of Bevacizumab on migration of human Tenon capsule fibroblasts
AIM: To investigate the inhibition effect of bevacizumab on human Tenon capsule fibroblasts(HTFs)and discuss the countermeasures of bleb scarringscarring in glaucoma surgery countermeasures. <p>METHODS: Adopted cell recovery method and followed the aseptic principles, we performed the culture...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2015-01-01
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| Series: | Guoji Yanke Zazhi |
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| Online Access: | http://ies.ijo.cn/cn_publish/2015/1/201501008.pdf |
| _version_ | 1818411826992381952 |
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| author | Jing Li An-Ming Xie |
| author_facet | Jing Li An-Ming Xie |
| author_sort | Jing Li |
| collection | DOAJ |
| description | AIM: To investigate the inhibition effect of bevacizumab on human Tenon capsule fibroblasts(HTFs)and discuss the countermeasures of bleb scarringscarring in glaucoma surgery countermeasures. <p>METHODS: Adopted cell recovery method and followed the aseptic principles, we performed the culture of HTFs which came from the Central Laboratory of Shaanxi People's Hospital cell library. Wound Healing assay: We scraped a cell-free zone on the cell surface when the cells reached confluence at 80%. The control group was added to serum-free DMEM medium. The HTFs of bevacizumab group were stimulated with 1mg/mL concentrations without DEME for 0, 24, 48, and 72h. The scratch width was observed and measured.<p>RESULTS:HTFs were long fusiform shape under microscope, the nucleus is in the center of the cell with larger nucleus, abundant cytoplasm, were arranged in a whorled growth out of shape, strong ability to proliferate, conform to general forms of fibroblast. The morphological and biological characteristics of cells after cryopreservation and resuscitation remain unchanged. Wound healing assay: 0h, equal to the initial width of the two groups, 24h when the migration distance of the two groups of cells are basically the same, 48h when the control group cell migration distance is greater than that in the bevacizumab processing group, 72h when the control group scratches basichealing, bevacizumab treated cells migrate closer than 48h no significant change, and a lot of cells died. <p>CONCLUSION:Cell recovery method can successfully cultured HTFs, which was stability on morphology and biological properties, laying the cellular basis for experimental research.Fibroblast itself has a strong ability to migrate, outside-derived bevacizumab can inhibit HTFs migration evidently and it will cause excessive cell death when. Bevacizumab has certain extent inhibitory effect on HTFs migration, and it is likely to become one of the important drugs for creating bleb scarring after glaucoma surgery in the future. |
| first_indexed | 2024-12-14T10:37:36Z |
| format | Article |
| id | doaj.art-e3be0dbc7e084d3b80def58123c07b02 |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-14T10:37:36Z |
| publishDate | 2015-01-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-e3be0dbc7e084d3b80def58123c07b022022-12-21T23:05:51ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232015-01-01151343710.3980/j.issn.1672-5123.2015.1.08Inhibition effects of Bevacizumab on migration of human Tenon capsule fibroblastsJing Li0An-Ming Xie1Department of Ophthalmology, Shaanxi Provincial People's Hospital, Xi'an 710061, Shaanxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, ChinaAIM: To investigate the inhibition effect of bevacizumab on human Tenon capsule fibroblasts(HTFs)and discuss the countermeasures of bleb scarringscarring in glaucoma surgery countermeasures. <p>METHODS: Adopted cell recovery method and followed the aseptic principles, we performed the culture of HTFs which came from the Central Laboratory of Shaanxi People's Hospital cell library. Wound Healing assay: We scraped a cell-free zone on the cell surface when the cells reached confluence at 80%. The control group was added to serum-free DMEM medium. The HTFs of bevacizumab group were stimulated with 1mg/mL concentrations without DEME for 0, 24, 48, and 72h. The scratch width was observed and measured.<p>RESULTS:HTFs were long fusiform shape under microscope, the nucleus is in the center of the cell with larger nucleus, abundant cytoplasm, were arranged in a whorled growth out of shape, strong ability to proliferate, conform to general forms of fibroblast. The morphological and biological characteristics of cells after cryopreservation and resuscitation remain unchanged. Wound healing assay: 0h, equal to the initial width of the two groups, 24h when the migration distance of the two groups of cells are basically the same, 48h when the control group cell migration distance is greater than that in the bevacizumab processing group, 72h when the control group scratches basichealing, bevacizumab treated cells migrate closer than 48h no significant change, and a lot of cells died. <p>CONCLUSION:Cell recovery method can successfully cultured HTFs, which was stability on morphology and biological properties, laying the cellular basis for experimental research.Fibroblast itself has a strong ability to migrate, outside-derived bevacizumab can inhibit HTFs migration evidently and it will cause excessive cell death when. Bevacizumab has certain extent inhibitory effect on HTFs migration, and it is likely to become one of the important drugs for creating bleb scarring after glaucoma surgery in the future.http://ies.ijo.cn/cn_publish/2015/1/201501008.pdfhuman Tenon capsule fibroblastsbevacizumabmigrationbleb scarring |
| spellingShingle | Jing Li An-Ming Xie Inhibition effects of Bevacizumab on migration of human Tenon capsule fibroblasts Guoji Yanke Zazhi human Tenon capsule fibroblasts bevacizumab migration bleb scarring |
| title | Inhibition effects of Bevacizumab on migration of human Tenon capsule fibroblasts |
| title_full | Inhibition effects of Bevacizumab on migration of human Tenon capsule fibroblasts |
| title_fullStr | Inhibition effects of Bevacizumab on migration of human Tenon capsule fibroblasts |
| title_full_unstemmed | Inhibition effects of Bevacizumab on migration of human Tenon capsule fibroblasts |
| title_short | Inhibition effects of Bevacizumab on migration of human Tenon capsule fibroblasts |
| title_sort | inhibition effects of bevacizumab on migration of human tenon capsule fibroblasts |
| topic | human Tenon capsule fibroblasts bevacizumab migration bleb scarring |
| url | http://ies.ijo.cn/cn_publish/2015/1/201501008.pdf |
| work_keys_str_mv | AT jingli inhibitioneffectsofbevacizumabonmigrationofhumantenoncapsulefibroblasts AT anmingxie inhibitioneffectsofbevacizumabonmigrationofhumantenoncapsulefibroblasts |