Effect of antibiotic monensin on cell proliferation and IGF1R signaling pathway in human colorectal cancer cells
AbstractBackground/Aims Colorectal cancer is the third leading cause of death in patients with cancers in America. Monensin has represented anti-cancer effect on various human cancer cells. We seek to investigate the effect of monensin on proliferation of human colorectal cancer cells and explore wh...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Annals of Medicine |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07853890.2023.2166980 |
| _version_ | 1827380503128309760 |
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| author | Youping Zhou Youlin Deng Jing Wang Zhengjian Yan Qiang Wei Jixing Ye Junhui Zhang Tong-Chuan He Min Qiao |
| author_facet | Youping Zhou Youlin Deng Jing Wang Zhengjian Yan Qiang Wei Jixing Ye Junhui Zhang Tong-Chuan He Min Qiao |
| author_sort | Youping Zhou |
| collection | DOAJ |
| description | AbstractBackground/Aims Colorectal cancer is the third leading cause of death in patients with cancers in America. Monensin has represented anti-cancer effect on various human cancer cells. We seek to investigate the effect of monensin on proliferation of human colorectal cancer cells and explore whether IGF1R signaling pathway is involved in anti-cancer mechanism of monensin.Methods Cell proliferation and migration were assessed by crystal violet staining and cell wounding assay respectively. Cell apoptosis was analyzed by Hoechst 33258 staining and flow cytometry. Cell cycle progression was detected with the use of flow cytometry. Cancer-associated pathways were assessed with the use of pathway-specific reporters. Gene expression was detected by touchdown-quantitative real-time PCR. Inhibition of IGF1R was tested by immunofluorescence staining. Inhibition of IGF1R signaling was accomplished by adenovirus-mediated expression of IGF1.Results We found that monensin not only effectively inhibited cell proliferation, cell migration as well as cell cycle progression, but also induced apoptosis and G1 arrest in human colorectal cancer cells. Monensin was shown to target multiple cancer-related signaling pathways such as Elk1, AP1, as well as Myc/max, and suppressed IGF1R expression via increasing IGF1 in colorectal cancer cells.Conclusion Monensin could suppressed IGF1R expression via increasing IGF1 in colorectal cancer cells. It has the potential to be repurposed as an anti-colorectal cancer agent, but further studies are still required to investigate the detailed mechanisms of monensin underlying its anti-cancer motion.Key MessagesMonensin inhibits the cell proliferation and the migration, induces apoptosis and inhibits cell cycle progression in human colorectal cancer cells.Monensin may exert anti-cancer activity by targeting multiple signaling pathways, including the IGF1R signaling pathway.Monensin has the potential to be repurposed as an anti-colorectal cancer agent. |
| first_indexed | 2024-03-08T13:34:50Z |
| format | Article |
| id | doaj.art-e3c1879e04364a45ab0961e50f4a816a |
| institution | Directory Open Access Journal |
| issn | 0785-3890 1365-2060 |
| language | English |
| last_indexed | 2024-03-08T13:34:50Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Medicine |
| spelling | doaj.art-e3c1879e04364a45ab0961e50f4a816a2024-01-16T19:13:22ZengTaylor & Francis GroupAnnals of Medicine0785-38901365-20602023-12-0155195496410.1080/07853890.2023.2166980Effect of antibiotic monensin on cell proliferation and IGF1R signaling pathway in human colorectal cancer cellsYouping Zhou0Youlin Deng1Jing Wang2Zhengjian Yan3Qiang Wei4Jixing Ye5Junhui Zhang6Tong-Chuan He7Min Qiao8Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, USAMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, USAMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, USAMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, USAMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, USAMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, USAMolecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, USADepartment of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaAbstractBackground/Aims Colorectal cancer is the third leading cause of death in patients with cancers in America. Monensin has represented anti-cancer effect on various human cancer cells. We seek to investigate the effect of monensin on proliferation of human colorectal cancer cells and explore whether IGF1R signaling pathway is involved in anti-cancer mechanism of monensin.Methods Cell proliferation and migration were assessed by crystal violet staining and cell wounding assay respectively. Cell apoptosis was analyzed by Hoechst 33258 staining and flow cytometry. Cell cycle progression was detected with the use of flow cytometry. Cancer-associated pathways were assessed with the use of pathway-specific reporters. Gene expression was detected by touchdown-quantitative real-time PCR. Inhibition of IGF1R was tested by immunofluorescence staining. Inhibition of IGF1R signaling was accomplished by adenovirus-mediated expression of IGF1.Results We found that monensin not only effectively inhibited cell proliferation, cell migration as well as cell cycle progression, but also induced apoptosis and G1 arrest in human colorectal cancer cells. Monensin was shown to target multiple cancer-related signaling pathways such as Elk1, AP1, as well as Myc/max, and suppressed IGF1R expression via increasing IGF1 in colorectal cancer cells.Conclusion Monensin could suppressed IGF1R expression via increasing IGF1 in colorectal cancer cells. It has the potential to be repurposed as an anti-colorectal cancer agent, but further studies are still required to investigate the detailed mechanisms of monensin underlying its anti-cancer motion.Key MessagesMonensin inhibits the cell proliferation and the migration, induces apoptosis and inhibits cell cycle progression in human colorectal cancer cells.Monensin may exert anti-cancer activity by targeting multiple signaling pathways, including the IGF1R signaling pathway.Monensin has the potential to be repurposed as an anti-colorectal cancer agent.https://www.tandfonline.com/doi/10.1080/07853890.2023.2166980Colorectal cancermonensincell proliferationIGF signalingcancer therapy |
| spellingShingle | Youping Zhou Youlin Deng Jing Wang Zhengjian Yan Qiang Wei Jixing Ye Junhui Zhang Tong-Chuan He Min Qiao Effect of antibiotic monensin on cell proliferation and IGF1R signaling pathway in human colorectal cancer cells Annals of Medicine Colorectal cancer monensin cell proliferation IGF signaling cancer therapy |
| title | Effect of antibiotic monensin on cell proliferation and IGF1R signaling pathway in human colorectal cancer cells |
| title_full | Effect of antibiotic monensin on cell proliferation and IGF1R signaling pathway in human colorectal cancer cells |
| title_fullStr | Effect of antibiotic monensin on cell proliferation and IGF1R signaling pathway in human colorectal cancer cells |
| title_full_unstemmed | Effect of antibiotic monensin on cell proliferation and IGF1R signaling pathway in human colorectal cancer cells |
| title_short | Effect of antibiotic monensin on cell proliferation and IGF1R signaling pathway in human colorectal cancer cells |
| title_sort | effect of antibiotic monensin on cell proliferation and igf1r signaling pathway in human colorectal cancer cells |
| topic | Colorectal cancer monensin cell proliferation IGF signaling cancer therapy |
| url | https://www.tandfonline.com/doi/10.1080/07853890.2023.2166980 |
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