Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components
Summary Fungi produce a wide range of enzymes that allow them to grow on diverse plant biomass. Wheat bran is a low‐cost substrate with high potential for biotechnological applications. It mainly contains cellulose and (arabino)xylan, as well as starch, proteins, lipids and lignin to a lesser extent...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-07-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13835 |
| _version_ | 1818655752703705088 |
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| author | Roland S. Kun Sandra Garrigues Marcos Di Falco Adrian Tsang Ronald P. deVries |
| author_facet | Roland S. Kun Sandra Garrigues Marcos Di Falco Adrian Tsang Ronald P. deVries |
| author_sort | Roland S. Kun |
| collection | DOAJ |
| description | Summary Fungi produce a wide range of enzymes that allow them to grow on diverse plant biomass. Wheat bran is a low‐cost substrate with high potential for biotechnological applications. It mainly contains cellulose and (arabino)xylan, as well as starch, proteins, lipids and lignin to a lesser extent. In this study, we dissected the regulatory network governing wheat bran degradation in Aspergillus niger to assess the relative contribution of the regulators to the utilization of this plant biomass substrate. Deletion of genes encoding transcription factors involved in (hemi‐)cellulose utilization (XlnR, AraR, ClrA and ClrB) individually and in combination significantly reduced production of polysaccharide‐degrading enzymes, but retained substantial growth on wheat bran. Proteomic analysis suggested the ability of A. niger to grow on other carbon components, such as starch, which was confirmed by the additional deletion of the amylolytic regulator AmyR. Growth was further reduced but not impaired, indicating that other minor components provide sufficient energy for residual growth, displaying the flexibility of A. niger, and likely other fungi, in carbon utilization. Better understanding of the complexity and flexibility of fungal regulatory networks will facilitate the generation of more efficient fungal cell factories that use plant biomass as a substrate. |
| first_indexed | 2024-12-17T03:14:41Z |
| format | Article |
| id | doaj.art-e3c66e5f6ddf4ed89f8aece59c7626e5 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-17T03:14:41Z |
| publishDate | 2021-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-e3c66e5f6ddf4ed89f8aece59c7626e52022-12-21T22:05:43ZengWileyMicrobial Biotechnology1751-79152021-07-011441683169810.1111/1751-7915.13835Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor componentsRoland S. Kun0Sandra Garrigues1Marcos Di Falco2Adrian Tsang3Ronald P. deVries4Fungal Physiology Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology Utrecht University Uppsalalaan 8 Utrecht3584 CTThe NetherlandsFungal Physiology Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology Utrecht University Uppsalalaan 8 Utrecht3584 CTThe NetherlandsCentre for Structural and Functional Genomics Concordia University 7141 Sherbrooke Street West Montreal QCH4B 1R6CanadaCentre for Structural and Functional Genomics Concordia University 7141 Sherbrooke Street West Montreal QCH4B 1R6CanadaFungal Physiology Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology Utrecht University Uppsalalaan 8 Utrecht3584 CTThe NetherlandsSummary Fungi produce a wide range of enzymes that allow them to grow on diverse plant biomass. Wheat bran is a low‐cost substrate with high potential for biotechnological applications. It mainly contains cellulose and (arabino)xylan, as well as starch, proteins, lipids and lignin to a lesser extent. In this study, we dissected the regulatory network governing wheat bran degradation in Aspergillus niger to assess the relative contribution of the regulators to the utilization of this plant biomass substrate. Deletion of genes encoding transcription factors involved in (hemi‐)cellulose utilization (XlnR, AraR, ClrA and ClrB) individually and in combination significantly reduced production of polysaccharide‐degrading enzymes, but retained substantial growth on wheat bran. Proteomic analysis suggested the ability of A. niger to grow on other carbon components, such as starch, which was confirmed by the additional deletion of the amylolytic regulator AmyR. Growth was further reduced but not impaired, indicating that other minor components provide sufficient energy for residual growth, displaying the flexibility of A. niger, and likely other fungi, in carbon utilization. Better understanding of the complexity and flexibility of fungal regulatory networks will facilitate the generation of more efficient fungal cell factories that use plant biomass as a substrate.https://doi.org/10.1111/1751-7915.13835 |
| spellingShingle | Roland S. Kun Sandra Garrigues Marcos Di Falco Adrian Tsang Ronald P. deVries Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components Microbial Biotechnology |
| title | Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components |
| title_full | Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components |
| title_fullStr | Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components |
| title_full_unstemmed | Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components |
| title_short | Blocking utilization of major plant biomass polysaccharides leads Aspergillus niger towards utilization of minor components |
| title_sort | blocking utilization of major plant biomass polysaccharides leads aspergillus niger towards utilization of minor components |
| url | https://doi.org/10.1111/1751-7915.13835 |
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