CeO2 Nanoparticles Seed Priming Increases Salicylic Acid Level and ROS Scavenging Ability to Improve Rapeseed Salt Tolerance

Abstract Soil salinity is a major issue limiting efficient crop production. Seed priming with nanomaterials (nanopriming) is a cost‐effective technology to improve seed germination under salinity; however, the underlying mechanisms still need to be explored. Here, polyacrylic acid coated nanoceria (...

Full description

Bibliographic Details
Main Authors: Mohammad Nauman Khan, Yanhui Li, Chengcheng Fu, Jin Hu, Linlin Chen, Jiasen Yan, Zaid Khan, Honghong Wu, Zhaohu Li
Format: Article
Language:English
Published: Wiley 2022-07-01
Series:Global Challenges
Subjects:
Online Access:https://doi.org/10.1002/gch2.202200025
_version_ 1818005992179236864
author Mohammad Nauman Khan
Yanhui Li
Chengcheng Fu
Jin Hu
Linlin Chen
Jiasen Yan
Zaid Khan
Honghong Wu
Zhaohu Li
author_facet Mohammad Nauman Khan
Yanhui Li
Chengcheng Fu
Jin Hu
Linlin Chen
Jiasen Yan
Zaid Khan
Honghong Wu
Zhaohu Li
author_sort Mohammad Nauman Khan
collection DOAJ
description Abstract Soil salinity is a major issue limiting efficient crop production. Seed priming with nanomaterials (nanopriming) is a cost‐effective technology to improve seed germination under salinity; however, the underlying mechanisms still need to be explored. Here, polyacrylic acid coated nanoceria (cerium oxide nanoparticles) (PNC, 9.2 nm, −38.7 mV) are synthesized and characterized. The results show that under salinity, PNC priming significantly increases rapeseed shoot length (41.5%), root length (93%), and seedling dry weight (78%) compared to the no‐nanoparticle (NNP) priming group. Confocal imaging results show that compared with NNP group, PNC priming significantly reduces reactive oxygen species (ROS) level in leaf (94.3% of H2O2, 56.4% of •O2−) and root (38.4% of H2O2, 41.3% of •O2−) of salt stressed rapeseed seedlings. Further, the results show that compared with the NNP group, PNC priming not only increases salicylic acid (SA) content in shoot (51.3%) and root (78.4%), but also upregulates the expression of SA biosynthesis related genes in salt stressed rapeseed. Overall, PNC nanopriming improved rapeseed salt tolerance is associated with both the increase of ROS scavenging ability and the increase of salicylic acid. The results add more information to understand the complexity of mechanisms behind nanoceria priming improved plant salt tolerance.
first_indexed 2024-04-14T04:54:23Z
format Article
id doaj.art-e3c6f6b3103d467a948a9eecc6c9ee4b
institution Directory Open Access Journal
issn 2056-6646
language English
last_indexed 2024-04-14T04:54:23Z
publishDate 2022-07-01
publisher Wiley
record_format Article
series Global Challenges
spelling doaj.art-e3c6f6b3103d467a948a9eecc6c9ee4b2022-12-22T02:11:12ZengWileyGlobal Challenges2056-66462022-07-0167n/an/a10.1002/gch2.202200025CeO2 Nanoparticles Seed Priming Increases Salicylic Acid Level and ROS Scavenging Ability to Improve Rapeseed Salt ToleranceMohammad Nauman Khan0Yanhui Li1Chengcheng Fu2Jin Hu3Linlin Chen4Jiasen Yan5Zaid Khan6Honghong Wu7Zhaohu Li8MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 ChinaMOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 ChinaAbstract Soil salinity is a major issue limiting efficient crop production. Seed priming with nanomaterials (nanopriming) is a cost‐effective technology to improve seed germination under salinity; however, the underlying mechanisms still need to be explored. Here, polyacrylic acid coated nanoceria (cerium oxide nanoparticles) (PNC, 9.2 nm, −38.7 mV) are synthesized and characterized. The results show that under salinity, PNC priming significantly increases rapeseed shoot length (41.5%), root length (93%), and seedling dry weight (78%) compared to the no‐nanoparticle (NNP) priming group. Confocal imaging results show that compared with NNP group, PNC priming significantly reduces reactive oxygen species (ROS) level in leaf (94.3% of H2O2, 56.4% of •O2−) and root (38.4% of H2O2, 41.3% of •O2−) of salt stressed rapeseed seedlings. Further, the results show that compared with the NNP group, PNC priming not only increases salicylic acid (SA) content in shoot (51.3%) and root (78.4%), but also upregulates the expression of SA biosynthesis related genes in salt stressed rapeseed. Overall, PNC nanopriming improved rapeseed salt tolerance is associated with both the increase of ROS scavenging ability and the increase of salicylic acid. The results add more information to understand the complexity of mechanisms behind nanoceria priming improved plant salt tolerance.https://doi.org/10.1002/gch2.202200025gene expressionnanoceria seed primingROS scavengingsalicylic acidsalinity
spellingShingle Mohammad Nauman Khan
Yanhui Li
Chengcheng Fu
Jin Hu
Linlin Chen
Jiasen Yan
Zaid Khan
Honghong Wu
Zhaohu Li
CeO2 Nanoparticles Seed Priming Increases Salicylic Acid Level and ROS Scavenging Ability to Improve Rapeseed Salt Tolerance
Global Challenges
gene expression
nanoceria seed priming
ROS scavenging
salicylic acid
salinity
title CeO2 Nanoparticles Seed Priming Increases Salicylic Acid Level and ROS Scavenging Ability to Improve Rapeseed Salt Tolerance
title_full CeO2 Nanoparticles Seed Priming Increases Salicylic Acid Level and ROS Scavenging Ability to Improve Rapeseed Salt Tolerance
title_fullStr CeO2 Nanoparticles Seed Priming Increases Salicylic Acid Level and ROS Scavenging Ability to Improve Rapeseed Salt Tolerance
title_full_unstemmed CeO2 Nanoparticles Seed Priming Increases Salicylic Acid Level and ROS Scavenging Ability to Improve Rapeseed Salt Tolerance
title_short CeO2 Nanoparticles Seed Priming Increases Salicylic Acid Level and ROS Scavenging Ability to Improve Rapeseed Salt Tolerance
title_sort ceo2 nanoparticles seed priming increases salicylic acid level and ros scavenging ability to improve rapeseed salt tolerance
topic gene expression
nanoceria seed priming
ROS scavenging
salicylic acid
salinity
url https://doi.org/10.1002/gch2.202200025
work_keys_str_mv AT mohammadnaumankhan ceo2nanoparticlesseedprimingincreasessalicylicacidlevelandrosscavengingabilitytoimproverapeseedsalttolerance
AT yanhuili ceo2nanoparticlesseedprimingincreasessalicylicacidlevelandrosscavengingabilitytoimproverapeseedsalttolerance
AT chengchengfu ceo2nanoparticlesseedprimingincreasessalicylicacidlevelandrosscavengingabilitytoimproverapeseedsalttolerance
AT jinhu ceo2nanoparticlesseedprimingincreasessalicylicacidlevelandrosscavengingabilitytoimproverapeseedsalttolerance
AT linlinchen ceo2nanoparticlesseedprimingincreasessalicylicacidlevelandrosscavengingabilitytoimproverapeseedsalttolerance
AT jiasenyan ceo2nanoparticlesseedprimingincreasessalicylicacidlevelandrosscavengingabilitytoimproverapeseedsalttolerance
AT zaidkhan ceo2nanoparticlesseedprimingincreasessalicylicacidlevelandrosscavengingabilitytoimproverapeseedsalttolerance
AT honghongwu ceo2nanoparticlesseedprimingincreasessalicylicacidlevelandrosscavengingabilitytoimproverapeseedsalttolerance
AT zhaohuli ceo2nanoparticlesseedprimingincreasessalicylicacidlevelandrosscavengingabilitytoimproverapeseedsalttolerance