In vitro callus co-cultivation strategy for prospecting growth regulators to induce in vivo grapevine graft union
Abstract The use of growth regulators (GRs) in a mechanized bench grafting system is essential for the final quality of grafted grapevine plants. In Brazil, the GRs used (commercial waxes) are imported, increasing the production cost for nurseries. This work aimed to test the in vitro callus co-cult...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Fruticultura
2024-04-01
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| Series: | Revista Brasileira de Fruticultura |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452024000101702&lng=en&tlng=en |
| _version_ | 1797205771663966208 |
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| author | Daniel Santos Grohs Henrique Pessoa dos Santos Paulo Vitor Dutra de Souza Mikael Marcio Benati |
| author_facet | Daniel Santos Grohs Henrique Pessoa dos Santos Paulo Vitor Dutra de Souza Mikael Marcio Benati |
| author_sort | Daniel Santos Grohs |
| collection | DOAJ |
| description | Abstract The use of growth regulators (GRs) in a mechanized bench grafting system is essential for the final quality of grafted grapevine plants. In Brazil, the GRs used (commercial waxes) are imported, increasing the production cost for nurseries. This work aimed to test the in vitro callus co-cultivation technique as a strategy for prospecting effective doses and mixtures of GRs for in vivo graft union on grapevines. Two genotypes were used as scions (‘Bordo’ and ‘Riesling Italico’) and two as rootstocks (‘SO4’ and ‘P1103’). Callus from different scion/rootstock combinations were co-cultivated, varying the doses (0.25 to 20 mg l-1) of auxins (DBA, IAA and IBA) and cytokinin (BAP), following the Doehlert design. The best in vitro treatments were validated in vivo, in plants grafted with an ‘omega’ cutter machine. Multivariate and non-parametric methods were applied to integrate the results. In vitro prospecting combined with the in vivo validation strategy allowed us to select effectively the IAA(2.50 mg l-1) plus BAP(1.00 mg l-1) formulation with the potential to induce graft callus union similar to commercial waxes. The induction intensity varied among the genetic combinations but was similar in the in vitro and in vivo conditions. |
| first_indexed | 2024-04-24T08:56:25Z |
| format | Article |
| id | doaj.art-e3c8be5436e84afcbec94844e80caaa7 |
| institution | Directory Open Access Journal |
| issn | 1806-9967 |
| language | English |
| last_indexed | 2024-04-24T08:56:25Z |
| publishDate | 2024-04-01 |
| publisher | Sociedade Brasileira de Fruticultura |
| record_format | Article |
| series | Revista Brasileira de Fruticultura |
| spelling | doaj.art-e3c8be5436e84afcbec94844e80caaa72024-04-16T07:40:20ZengSociedade Brasileira de FruticulturaRevista Brasileira de Fruticultura1806-99672024-04-014610.1590/0100-29452024522In vitro callus co-cultivation strategy for prospecting growth regulators to induce in vivo grapevine graft unionDaniel Santos Grohshttps://orcid.org/0000-0002-6712-3166Henrique Pessoa dos Santoshttps://orcid.org/0000-0003-4066-1463Paulo Vitor Dutra de Souzahttps://orcid.org/0000-0003-2900-3638Mikael Marcio Benatihttps://orcid.org/0000-0002-4105-4596Abstract The use of growth regulators (GRs) in a mechanized bench grafting system is essential for the final quality of grafted grapevine plants. In Brazil, the GRs used (commercial waxes) are imported, increasing the production cost for nurseries. This work aimed to test the in vitro callus co-cultivation technique as a strategy for prospecting effective doses and mixtures of GRs for in vivo graft union on grapevines. Two genotypes were used as scions (‘Bordo’ and ‘Riesling Italico’) and two as rootstocks (‘SO4’ and ‘P1103’). Callus from different scion/rootstock combinations were co-cultivated, varying the doses (0.25 to 20 mg l-1) of auxins (DBA, IAA and IBA) and cytokinin (BAP), following the Doehlert design. The best in vitro treatments were validated in vivo, in plants grafted with an ‘omega’ cutter machine. Multivariate and non-parametric methods were applied to integrate the results. In vitro prospecting combined with the in vivo validation strategy allowed us to select effectively the IAA(2.50 mg l-1) plus BAP(1.00 mg l-1) formulation with the potential to induce graft callus union similar to commercial waxes. The induction intensity varied among the genetic combinations but was similar in the in vitro and in vivo conditions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452024000101702&lng=en&tlng=enVitis spp.micropropagationgraftingphytohormonesnurseries |
| spellingShingle | Daniel Santos Grohs Henrique Pessoa dos Santos Paulo Vitor Dutra de Souza Mikael Marcio Benati In vitro callus co-cultivation strategy for prospecting growth regulators to induce in vivo grapevine graft union Revista Brasileira de Fruticultura Vitis spp. micropropagation grafting phytohormones nurseries |
| title | In vitro callus co-cultivation strategy for prospecting growth regulators to induce in vivo grapevine graft union |
| title_full | In vitro callus co-cultivation strategy for prospecting growth regulators to induce in vivo grapevine graft union |
| title_fullStr | In vitro callus co-cultivation strategy for prospecting growth regulators to induce in vivo grapevine graft union |
| title_full_unstemmed | In vitro callus co-cultivation strategy for prospecting growth regulators to induce in vivo grapevine graft union |
| title_short | In vitro callus co-cultivation strategy for prospecting growth regulators to induce in vivo grapevine graft union |
| title_sort | in vitro callus co cultivation strategy for prospecting growth regulators to induce in vivo grapevine graft union |
| topic | Vitis spp. micropropagation grafting phytohormones nurseries |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452024000101702&lng=en&tlng=en |
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