Dual Response Site Fluorescent Probe for Highly Sensitive Detection of Cys/Hcy and GSH In Vivo through Two Different Emission Channels
Much research has demonstrated that metabolic imbalances of biothiols are closely associated with the emergence of different types of disease. In view of the significant effect of biothiols, quantitative evaluation and discrimination of intracellular Cys/Hcy and GSH in complex biological environment...
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MDPI AG
2022-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/11/1056 |
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| author | Huiling Hou Qi Liu Xiangbao Liu Shuang Fu Hongguang Zhang Shuang Li Song Chen Peng Hou |
| author_facet | Huiling Hou Qi Liu Xiangbao Liu Shuang Fu Hongguang Zhang Shuang Li Song Chen Peng Hou |
| author_sort | Huiling Hou |
| collection | DOAJ |
| description | Much research has demonstrated that metabolic imbalances of biothiols are closely associated with the emergence of different types of disease. In view of the significant effect of biothiols, quantitative evaluation and discrimination of intracellular Cys/Hcy and GSH in complex biological environments is very important. In this study, probe <b>CDS-NBD</b>, synthesized by attaching 2,4-dinitrobenzenesulfonate (DNBS, site 1) and nitrobenzoxadiazole (NBD, site 2) as the highly sensitive and selective dual response site for thiols onto the coumarin derivative 7-hydroxycoumarin-4-acetic acid, exhibited large separation of the emission wavelengths, fast response, notable fluorescence enhancement, excellent sensitivity and selectivity to Cys/Hcy and GSH over other biological species. Additionally, <b>CDS-NBD</b> could make a distinction between two different fluorescent signals, GSH (an obvious blue fluorescence) and Cys/Hcy (a mixed blue-green fluorescence). Further study on imaging of Cys/Hcy and GSH in vivo by employing probe <b>CDS-NBD</b> could also be successfully achieved. |
| first_indexed | 2024-03-09T18:27:52Z |
| format | Article |
| id | doaj.art-e3c9badd1d0a4fc899643ecb6cab9bf2 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T18:27:52Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-e3c9badd1d0a4fc899643ecb6cab9bf22023-11-24T07:49:13ZengMDPI AGBiosensors2079-63742022-11-011211105610.3390/bios12111056Dual Response Site Fluorescent Probe for Highly Sensitive Detection of Cys/Hcy and GSH In Vivo through Two Different Emission ChannelsHuiling Hou0Qi Liu1Xiangbao Liu2Shuang Fu3Hongguang Zhang4Shuang Li5Song Chen6Peng Hou7Achievement Transformation Center, Qiqihar Medical University, Qiqihar 161006, ChinaResearch Institute of Medicine & Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaCollege of Pharmacy, Qiqihar Medical University, Qiqihar 161006, ChinaMuch research has demonstrated that metabolic imbalances of biothiols are closely associated with the emergence of different types of disease. In view of the significant effect of biothiols, quantitative evaluation and discrimination of intracellular Cys/Hcy and GSH in complex biological environments is very important. In this study, probe <b>CDS-NBD</b>, synthesized by attaching 2,4-dinitrobenzenesulfonate (DNBS, site 1) and nitrobenzoxadiazole (NBD, site 2) as the highly sensitive and selective dual response site for thiols onto the coumarin derivative 7-hydroxycoumarin-4-acetic acid, exhibited large separation of the emission wavelengths, fast response, notable fluorescence enhancement, excellent sensitivity and selectivity to Cys/Hcy and GSH over other biological species. Additionally, <b>CDS-NBD</b> could make a distinction between two different fluorescent signals, GSH (an obvious blue fluorescence) and Cys/Hcy (a mixed blue-green fluorescence). Further study on imaging of Cys/Hcy and GSH in vivo by employing probe <b>CDS-NBD</b> could also be successfully achieved.https://www.mdpi.com/2079-6374/12/11/1056biothiolsdual-sitecoumarinfluorescent probe |
| spellingShingle | Huiling Hou Qi Liu Xiangbao Liu Shuang Fu Hongguang Zhang Shuang Li Song Chen Peng Hou Dual Response Site Fluorescent Probe for Highly Sensitive Detection of Cys/Hcy and GSH In Vivo through Two Different Emission Channels Biosensors biothiols dual-site coumarin fluorescent probe |
| title | Dual Response Site Fluorescent Probe for Highly Sensitive Detection of Cys/Hcy and GSH In Vivo through Two Different Emission Channels |
| title_full | Dual Response Site Fluorescent Probe for Highly Sensitive Detection of Cys/Hcy and GSH In Vivo through Two Different Emission Channels |
| title_fullStr | Dual Response Site Fluorescent Probe for Highly Sensitive Detection of Cys/Hcy and GSH In Vivo through Two Different Emission Channels |
| title_full_unstemmed | Dual Response Site Fluorescent Probe for Highly Sensitive Detection of Cys/Hcy and GSH In Vivo through Two Different Emission Channels |
| title_short | Dual Response Site Fluorescent Probe for Highly Sensitive Detection of Cys/Hcy and GSH In Vivo through Two Different Emission Channels |
| title_sort | dual response site fluorescent probe for highly sensitive detection of cys hcy and gsh in vivo through two different emission channels |
| topic | biothiols dual-site coumarin fluorescent probe |
| url | https://www.mdpi.com/2079-6374/12/11/1056 |
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