Comparative study of leucine on digestive enzyme secretion, ISR and mTOR pathway in cattle and porcine pancreas in vitro
ABSTRACTThis experiment is to investigate whether interspecies differences exist in the effects of leucine (Leu) on digestive enzyme secretion in pancreatic tissues through in vitro. Five healthy Simmental cattle and Duroc × Landrace × Yorkshire growing-finishing pigs were selected, and pancreatic t...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Applied Animal Research |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09712119.2024.2335942 |
| _version_ | 1797215859439042560 |
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| author | Zhanhe Zhang Xuan Ma Ru Li Mengya Wang Kai Shao Donglin Wu Ming Xu |
| author_facet | Zhanhe Zhang Xuan Ma Ru Li Mengya Wang Kai Shao Donglin Wu Ming Xu |
| author_sort | Zhanhe Zhang |
| collection | DOAJ |
| description | ABSTRACTThis experiment is to investigate whether interspecies differences exist in the effects of leucine (Leu) on digestive enzyme secretion in pancreatic tissues through in vitro. Five healthy Simmental cattle and Duroc × Landrace × Yorkshire growing-finishing pigs were selected, and pancreatic tissues were taken for culture after slaughter. Leu levels of 0, 2.62, 5.24, 10.48 mg/mL. Leu levels of 0, 5.24 mg/mL were selected to determine the expression of mRNA and proteins in the signaling pathways. The results showed that Leu increased the activity of digestive enzyme in cattle and pig, and there was an interaction was found on trypsin and chymotrypsin (P < 0.05). Leu had a significant effect on the mRNA (PKB, S6K1, 4E-BP1 and ATF4) and protein expression (PKB and 4E-BP1) and the phosphorylation (PKB, S6K1 and ATF4) levels (P < 0.05). Animal species had significant effects on the mRNA (PKB, S6K1 and ATF4) and protein (S6K1 and 4E-BP1) expression, and the phosphorylation (4E-BP1) levels (P < 0.05). There was an interaction between animal species and Leu on the expression of protein (PKB and S6K1) (P < 0.05). In summary, interspecies differences exist in the regulation of Leu on digestive enzyme secretion in pancreatic tissues in vitro of different animals. |
| first_indexed | 2024-04-24T11:36:46Z |
| format | Article |
| id | doaj.art-e3cbcaea48d94775bb12bc4d2d864f4e |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-04-24T11:36:46Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-e3cbcaea48d94775bb12bc4d2d864f4e2024-04-10T05:03:08ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442024-12-0152110.1080/09712119.2024.2335942Comparative study of leucine on digestive enzyme secretion, ISR and mTOR pathway in cattle and porcine pancreas in vitroZhanhe Zhang0Xuan Ma1Ru Li2Mengya Wang3Kai Shao4Donglin Wu5Ming Xu6College of Animal Science, Inner Mongolia Agricultural University, Hohhot, People’s Republic of ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, People’s Republic of ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, People’s Republic of ChinaCollege of Animal Science and Technology, Northwest A & F University, Yangling, People’s Republic of ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, People’s Republic of ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, People’s Republic of ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, People’s Republic of ChinaABSTRACTThis experiment is to investigate whether interspecies differences exist in the effects of leucine (Leu) on digestive enzyme secretion in pancreatic tissues through in vitro. Five healthy Simmental cattle and Duroc × Landrace × Yorkshire growing-finishing pigs were selected, and pancreatic tissues were taken for culture after slaughter. Leu levels of 0, 2.62, 5.24, 10.48 mg/mL. Leu levels of 0, 5.24 mg/mL were selected to determine the expression of mRNA and proteins in the signaling pathways. The results showed that Leu increased the activity of digestive enzyme in cattle and pig, and there was an interaction was found on trypsin and chymotrypsin (P < 0.05). Leu had a significant effect on the mRNA (PKB, S6K1, 4E-BP1 and ATF4) and protein expression (PKB and 4E-BP1) and the phosphorylation (PKB, S6K1 and ATF4) levels (P < 0.05). Animal species had significant effects on the mRNA (PKB, S6K1 and ATF4) and protein (S6K1 and 4E-BP1) expression, and the phosphorylation (4E-BP1) levels (P < 0.05). There was an interaction between animal species and Leu on the expression of protein (PKB and S6K1) (P < 0.05). In summary, interspecies differences exist in the regulation of Leu on digestive enzyme secretion in pancreatic tissues in vitro of different animals.https://www.tandfonline.com/doi/10.1080/09712119.2024.2335942Leucinedigestive enzymespancreasmTORISR |
| spellingShingle | Zhanhe Zhang Xuan Ma Ru Li Mengya Wang Kai Shao Donglin Wu Ming Xu Comparative study of leucine on digestive enzyme secretion, ISR and mTOR pathway in cattle and porcine pancreas in vitro Journal of Applied Animal Research Leucine digestive enzymes pancreas mTOR ISR |
| title | Comparative study of leucine on digestive enzyme secretion, ISR and mTOR pathway in cattle and porcine pancreas in vitro |
| title_full | Comparative study of leucine on digestive enzyme secretion, ISR and mTOR pathway in cattle and porcine pancreas in vitro |
| title_fullStr | Comparative study of leucine on digestive enzyme secretion, ISR and mTOR pathway in cattle and porcine pancreas in vitro |
| title_full_unstemmed | Comparative study of leucine on digestive enzyme secretion, ISR and mTOR pathway in cattle and porcine pancreas in vitro |
| title_short | Comparative study of leucine on digestive enzyme secretion, ISR and mTOR pathway in cattle and porcine pancreas in vitro |
| title_sort | comparative study of leucine on digestive enzyme secretion isr and mtor pathway in cattle and porcine pancreas in vitro |
| topic | Leucine digestive enzymes pancreas mTOR ISR |
| url | https://www.tandfonline.com/doi/10.1080/09712119.2024.2335942 |
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